Pulsed electric field treatment of saccharomyces cerevisiae using different waveforms

Qin, Si; Timoshkin, I. V.; Maclean, Michelle; Wilson, M. P.; Given, M.J.; Wang, Tao; Anderson, John G.; MacGregor, S.J.

doi:10.1109/tdei.2015.005152

Cited by 20 publications

(26 citation statements)

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“…Under atmospheric pressure, an electrical breakdown event is likely to occur across the top liquid-air interface, due to the short (1 mm) gap between the electrodes. To avoid this undesirable breakdown, the PEF treatment cell was placed inside a sealed metallic chamber, described in [12], which was pressurised up to 7.5 bar (absolute pressure) with compressed air. The elevated air pressure suppresses the development of interfacial spark breakdowns.…”

Section: B Pef System and Voltage Waveformsmentioning

confidence: 99%

“…In this group, the samples were placed inside the treatment cell for the same period of time without PEF treatment, to detect any antimicrobial effect. The treated and untreated (control) yeast samples were plated onto malt extract agar (Oxoid Ltd, UK) using spiral-and spread-plating methods, as detailed in [12]. Agar plates were then incubated at 30 °C for 20 hours.…”

Section: Assessment Of Pef Performancementioning

confidence: 99%

“…The inactivation kinetics and energy consumption for the PEF process performed in the treatment cell with TiO 2 -coated electrodes have been obtained. The results of PEF inactivation obtained using the treatment cell with TiO 2 -coated electrodes are compared with recently published results [12]: the results of the PEF treatment obtained using the treatment cell with uncoated conductive electrodes, and the same high-voltage (HV) waveforms.…”

Section: Introductionmentioning

confidence: 96%

“…In order to provide a direct comparison between this TiO 2 -coated and traditional metallic PEF treatment cells, the PEF treatment procedure was identical to the procedure used in [12], where the same microorganisms were subjected to pulsed electric fields in a treatment cell with uncoated, conductive electrodes. In [12], voltage impulses with two different waveshapes, square and exponential, and two peak electrical-field magnitudes, 67 kV/cm and 80 kV/cm, were used. In order to make comparison with this test cell, identical field strengths and waveshapes were used in the present study.…”

Section: Introductionmentioning

confidence: 99%

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TiO₂-Coated Electrodes for Pulsed Electric Field Treatment of Microorganisms

Qin

Timoshkin

Maclean

et al. 2016

IEEE Trans. Plasma Sci.

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Pulsed electric fields (PEF) can cause irreversible damage to bio-membranes and may result in inactivation of microorganisms. The aim of this paper is to investigate the PEF treatment of the yeast Saccharomyces cerevisiae, using a novel treatment cell with parallel-plane electrodes coated with a 2 µm thin TiO 2 film. Two different PEF waveforms, square and exponential, with magnitudes of 67 kV/cm and 80 kV/cm, were used in this study. The efficacy of the PEF treatment was assessed by comparison of the surviving treated and untreated yeast populations, and it was shown that a treatment cell with TiO 2-coated electrodes can be successfully used for the PEF treatment of microorganisms: 3-log 10 reduction in the yeast population was achieved with 100 impulses. The energy efficacy of the PEF process in the proposed treatment cell has been compared with the energy losses in the PEF treatment cell with uncoated, conductive electrodes. It is shown that the electrodes coated with TiO 2 provide better performance as compared with the traditional uncoated electrodes. Index Terms-Pulsed electric field, TiO 2-coated electrodes, inactivation of microorganisms, yeast.

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Section: B Pef System and Voltage Waveformsmentioning

confidence: 99%

Section: Assessment Of Pef Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TiO₂-Coated Electrodes for Pulsed Electric Field Treatment of Microorganisms

Qin

Timoshkin

Maclean

et al. 2016

IEEE Trans. Plasma Sci.

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“…The configuration of electrodes and the electric field distribution inside the PEF treatment chamber is supposed to have a greater impact on bacterial inactivation. There are many electrode configurations available where parallel plate electrodes were used in initial studies, and stainless steel was used as the electrode material for most of the PEF applications [3][4][5]. The electric field distribution inside the PEF chamber depends on the configuration of the electrodes.…”

Section: Introductionmentioning

confidence: 99%

Electric field Analysis of Water Electrodes for Noninvasive Pulsed Electric field Applications

Ramaswamy¹,

Prabu²,

Gowrisree³

2019

IJITEE

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Electroporation is an effective phenomenon of inactivating viable pathogens present in the liquid food for pulsed electric field (PEF) applications. It is a technique which depends on applied electric field strength for causing pores on cell plasma membrane. The various parameters which affect the electroporation efficacy are, the electric field intensity, pulse width, number of pulses, pulse interval and the electrode. The electrode provides a contact between the high voltage pulse generator and the liquid food, and it plays an important role in getting the required inactivation outcome. The electric field distribution varies based on electrode designs. Parallel plate electrodes are generally used due to the uniform electric field it delivers in the inactivation area, where high possibility of microbial inactivation will occur. This paper analyses the effectiveness of round edged parallel plate electrodes immersed in water which provides uniform electric field distribution in the inactivation area. Analyses have been performed on electric field distribution through four kinds of materials such as glass, alumina, quartz and plexiglass, which contains these electrodes in the center filled with sterile water. The electrodes are circular, and edge smoothened and hence the field distribution is also analyzed on electrode edges. The distance between the electrodes including the surface material is kept at 5 mm. The diameter of the electrodes are 40 mm and the electric field simulations are implemented in ANSYS MAXWELL v 15.0. Based on results it is reported that alumina required less peak voltage for generating 20 kV/cm field strength (nominal field required for bacterial inactivation) when compared with other materials. Also alumina exhibited less reduction of field travelling through it, and resulted in 82% of field application in the inactivation area which is comparatively higher than other materials. The results indicate that alumina is highly recommended for future noninvasive pulsed electric field applications.

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