Pulsed Electrodeposition of HAP/CPG-BSA/CUR Nanocomposite on Titanium Metal for Potential Bone Regeneration

Abstract

Bioactive hydroxyapatite (HAP) composites are progressively predicted as successive materials in bone regeneration therapy. A nanostructured composite made from the mixture of hydroxyapatite, chitosan (C)polyethylene glycol (P)-gelatin (G), and bovine serum albumin (BSA) (HAP/CPG-BSA) was prepared using ultra probe sonication followed by lyophilization. The anti-inflammatory and antioxidant-rich curcumin (CUR) molecules were impregnated on HAP/CPG-BSA composite for self-repairing bone regeneration. The physicochemical morphology of HAP/CPG-BSA/ CUR composite was analyzed by FTIR, XRD, SEM, and TEM techniques. The 92.0% of CUR release was observed from HAP/CPG-BSA/CUR composite by UV−VIS spectroscopy at a λ max value of 420 nm. The in vitro studies of HAP/CPG-BSA/CUR against osteoblast-like (MG63) cells and fibroblast cells (L929) resulted from 90.0 and 91.0% of cell viability for significant improvement in osteoinduction, proliferation, and viability for bone regeneration. The in vivo results of bone-cell formation by the implantation suggest that HAP/CPG-BSA/CUR composite might be useful in applications of regeneration medicine. The outcome of this study shows the ability to favor bone regeneration and signifies that this may be a prospective substitute implant for elevated repairing of bone.

In Vivo Assessment of a Hydroxyapatite/κ-Carrageenan–Maleic Anhydride–Casein/Doxorubicin Composite-Coated Titanium Bone Implant

“…The in vitro discharge behavior of DOX from the DOX-loaded HAP/κ-CA-MA-CAS/DOX composite was studied vfollowing formula was used to calculate ia the dialysis membrane (MWCO of 12,000 Da) method in a phosphate buffer solution (PBS) with a pH of 7.4 . Then, 2 mL of the PBS solution containing 50 mg of the DOX-loaded composite was stirred at 100 rpm at 25 °C.…”

“…Cell differentiation was studied using human lung cancer (A549) cells purchased from the National Centre for Cell Science (NCCS), Pune, India. The A549 cells were seeded in 24-well plates containing Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS and penicillin of 100 U/mL of streptomycin (100 U/mL) (Gibco, Grand Island, CA, U.S.A.) and cultivated for 24 h at a density of 4 × 10 4 cells/well . Then, they were cocultured with HAP, κ-CA-MA, κ-CA-MA-CAS, HAP/κ-CA-MA-CAS, and HAP/κ-CA-MA-CAS/DOX composites, and human lung cancer (A549) cells were used as control.…”

“…The in vitro discharge behavior of DOX from the DOX-loaded HAP/κ-CA-MA-CAS/DOX composite was studied vfollowing formula was used to calculate ia the dialysis membrane (MWCO of 12,000 Da) method in a phosphate buffer solution (PBS) with a pH of 7.4. 31 Then, 2 mL of the PBS solution containing 50 mg of the DOX-loaded composite was stirred at 100 rpm at 25 °C. The supernatant solution was collected at different time intervals, the concentration of the DOX solution was measured at a λ max value of 265 nm using UV−visible spectroscopy (UV1800, Shimadzu, India), and an identical quantity of a new PBS medium was refilled in the reaction medium.…”

“…The A549 cells were seeded in 24-well plates containing Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS and penicillin of 100 U/mL of streptomycin (100 U/mL) (Gibco, Grand Island, CA, U.S.A.) and cultivated for 24 h at a density of 4 × 10 4 cells/well. 31 Then, they were cocultured with HAP, κ-CA-MA, κ-CA-MA-CAS, HAP/κ-CA-MA-CAS, and HAP/κ-CA-MA-CAS/DOX composites, and human lung cancer (A549) cells were used as control. The cells were incubated at 37 °C in CO 2 , and they were identified using MTT assay techniques.…”

In-vivo Investigations of Hydroxyapatite/Co-polymeric Composites Coated Titanium Plate for Bone Regeneration

“…Titanium and its alloys developed for enduring implant utilizing for their favorable excellent mechanical properties, corrosion resistance, as well as their low dangers, biocompatibility, and such as elevated potency, stability, and less heaviness (Teshima et al, 2012 ; Sumathra and Rajan, 2019 ). However, they need particular surface treatment to defeat problems such as less bio-functionality, toxicity, or immunogenicity (Geuli et al, 2017 ).…”