Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids

Effective manipulation of liquids on open surfaces without external energy input is indispensable for the advancement of point-of-care diagnostic devices. Open-surface microfluidics has the potential to benefit health care, especially in the developing world. This review highlights the prospects for harnessing capillary forces on surface-microfluidic platforms, chiefly by inducing smooth gradients or sharp steps of wettability on substrates, to elicit passive liquid transport and higher-order fluidic manipulations without off-the-chip energy sources. A broad spectrum of the recent progress in the emerging field of passive surface microfluidics is highlighted, and its promise for developing facile, low-cost, easy-to-operate microfluidic devices is discussed in light of recent applications, not only in the domain of biomedical microfluidics but also in the general areas of energy and water conservation.

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Section: Applicationsmentioning

confidence: 99%

Patterning Wettability for Open-Surface Fluidic Manipulation: Fundamentals and Applications

et al. 2022

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“…24 Concisely, the AuNPs can be converted into hot spots upon exposure to a specific wavelength of the electromagnetic spectrum, depending on their sizes. [25][26][27] This exclusive optical property has been used in different medical scopes, like photodynamic therapy of cancers. 28 The surface of the AuNPs can be functionalized with the organic and inorganic compounds that include the thiol (-SH) groups in their structures.…”

Section: Overviewmentioning

confidence: 99%

Synergies in antimicrobial treatment by a levofloxacin-loaded halloysite and gold nanoparticles with a conjugation to a cell-penetrating peptide

et al. 2022

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“…The methods are advantageous because of minimal chances of inducing harsh cellular responses such as unwanted immunogenic or chemical reactions, thereby improving delivery efficiencies. Electroporation [ [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] ], optoporation [ [22] , [23] , [24] , [25] , [26] , [27] , [28] ], magnetoporation [ [29] , [30] , [31] ], acoustoporation [ [32] , [33] , [34] , [35] ] and mechanoporation [ [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] ] are some of the most popular membrane disruption techniques. Although each of these methods have their own distinct features (elucidated in detail in Table 2 ), all of these, except mechanoporation, require an external energy field for membrane permeabilization.…”

Section: Introductionmentioning

confidence: 99%

“… Requires external energy field. >100,000 95 : 90 [ [22] , [23] , [24] , [25] , [26] , [27] , [28] ] 3. Magnetoporation - Magnetic field parameters - Vectors to permeabilize the cell membrane It can be used in vivo.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic mechanoporation for cellular delivery and analysis

Chakrabarty

Gupta

Illath

et al. 2022

Materials Today Bio

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Highly efficient intracellular delivery strategies are essential for developing therapeutic, diagnostic, biological, and various biomedical applications. The recent advancement of micro/nanotechnology has focused numerous researches towards developing microfluidic device-based strategies due to the associated high throughput delivery, cost-effectiveness, robustness, and biocompatible nature. The delivery strategies can be carrier-mediated or membrane disruption-based, where membrane disruption methods find popularity due to reduced toxicity, enhanced delivery efficiency, and cell viability. Among all of the membrane disruption techniques, the mechanoporation strategies are advantageous because of no external energy source required for membrane deformation, thereby achieving high delivery efficiencies and increased cell viability into different cell types with negligible toxicity. The past two decades have consequently seen a tremendous boost in mechanoporation-based research for intracellular delivery and cellular analysis. This article provides a brief review of the most recent developments on microfluidic-based mechanoporation strategies such as microinjection, nanoneedle arrays, cell-squeezing, and hydroporation techniques with their working principle, device fabrication, cellular delivery, and analysis. Moreover, a brief discussion of the different mechanoporation strategies integrated with other delivery methods has also been provided. Finally, the advantages, limitations, and future prospects of this technique are discussed compared to other intracellular delivery techniques.

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Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids

Abstract: Targeted intracellular delivery of biomolecules and therapeutic cargo enables controlled manipulation of cellular processes. Laser-based optoporation has emerged as a versatile, non-invasive technique that employs light-based transient physical disruption of...

Cited by 27 publications

References 42 publications

Patterning Wettability for Open-Surface Fluidic Manipulation: Fundamentals and Applications

Patterning Wettability for Open-Surface Fluidic Manipulation: Fundamentals and Applications

Synergies in antimicrobial treatment by a levofloxacin-loaded halloysite and gold nanoparticles with a conjugation to a cell-penetrating peptide

Microfluidic mechanoporation for cellular delivery and analysis

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