Pulsed-laser fluorescence detection in capillary zone electrophoresis of some banned substances in sport

González, Esther Rebollar; Montes, Ruth E.; Laserna, J.J.

doi:10.1016/0003-2670(93)80133-6

Cited by 26 publications

(6 citation statements)

References 14 publications

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“…The drug is hepatically metabolized by hydroxylation and subsequent immediate conjugation with sulfuric acid to its main metabolite hydroxytriamterene sulfate (STA). CE has been used previously for the screening of diuretics and other banned drugs including triamterene (TA) in plasma or urine using UV detection as well as fluorescence detection with a xenon arc lamp [107] or a pulsed nitrogen laser [108] as light source. However, these methods were only used for qualitative identification and the metabolite STA was not included.…”

Section: Triamterenementioning

confidence: 99%

Recent progress in pharmacokinetic applications of capillary electrophoresis

Lin

Chen

2003

Electrophoresis

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This review is a continuation of the previous reviews (Electrophoresis 1999, 20, 3259-3268; Electrophoresis 2001, 22, 4244-4248) to update the recent publications from 2001 to 2003 on pharmacokinetic studies using capillary electrophoresis (CE). During this period of time, CE remains as a unique analytical method for some studies, which would otherwise be limited by many factors, such as the sample volume, detection sensitivity, or separation power. It is particularly noticeable that the separation of chiral drugs in biological sample and the use of solid-phase extraction (SPE) as a simple and convenient means of sample preparation appear to become popular for CE-based assays. The use of CE for assessing complete pharmacokinetic information, however, did not show a significant growth during this period of time. In order to provide a broad range of view on how biological samples are analyzed by CE, this review will cover publications during the past two years on the use of CE for the analysis of drugs in biological fluids for general pharmacokinetic applications including drug monitoring and bioavailability studies.

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Section: Triamterenementioning

confidence: 99%

Recent progress in pharmacokinetic applications of capillary electrophoresis

Lin

Chen

2003

Electrophoresis

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“…Among these methods, RP HPLC with UV-detection is the most common one and has been adapted as the standard screening method for doping control. In recent years, CE methods for diuretics analysis have been developed in the mode of either CZE [7,8] or MEKC [9,10]. In addition to the benefits of high separation efficiency, short analysis time, and low operation cost, CE methods also have the potential to be realized in the microchip to achieve high-throughput parallel analysis.…”

Section: Introductionmentioning

confidence: 99%

Indirect laser-induced fluorescence detection of diuretics separated by capillary electrophoresis

Yang

Wang

Zhang³

2006

J. Sep. Sci.

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Indirect LIF detection was applied to the detection of four acidic diuretics separated by CZE. Semiconductor laser was employed to provide the stable excitation of 473 nm. With an optimized electrophoretic buffer system which contained 5 mM of triethylamine, 0.1 microM of fluorescein, and 5% of n-butanol, fast separation of four diuretics (ethacrynic acid, chlorthalidone, bendroflumethiazide, and bumetanide) can be performed within 3 min with the detection limits of 0.2-2 microg/mL. The impacts of buffer components including the concentrations of the electrolytes, fluorescence probe, and the organic additives were demonstrated. The method was applied for the detection of diuretics in urine. As an alternative way for the fast analysis of diuretics, this indirect detection method provided the technical support for future microchip performances, in which diuretics may be detected in the microchip by the common LIF detector without derivatization.

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“…Among these methods, RP HPLC with UV detection is the most common one and has been adapted as the standard screening method for doping control. In recent years, CE methods for diuretics analysis have been developed by applying CZE [18,19] or MEKC [20,21]. The transfer of CE methods to MCE is usually a straightforward task with the exception that the dominant UV-absorbance detection is not easily realized in MCE.…”

Section: Introductionmentioning

confidence: 99%

Fast quantitative determination of diuretic drugs in tablets and human urine by microchip electrophoresis with native fluorescence detection

Tolba

Belder

2007

Electrophoresis

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Microchip electrophoresis (MCE) with native fluorescence detection has been applied for the fast quantitative analysis of pharmaceutical formulations. For this purpose, methods for fast separation and sensitive detection of the unlabeled diuretic drugs, amiloride, triamterene, bendroflumethiazide (BFMTZ), and bumetanide were developed. An epifluorescence setup was used enabling the coupling of different lasers into a commercial fluorescence microscope. The detection sensitivity of different excitation light sources was compared utilizing either a HeCd laser (lambda(exc) = 325 nm), a frequency quadrupled Nd:YAG laser (lambda(exc) = 266 nm), or a mercury lamp (lambda(exc) = 330-380 nm). At optimal conditions using the HeCd laser, the drugs were separated within 15 s with LODs less than 1 mug/mL for the four compounds. A linear relationship between concentration and peak area was obtained in the concentration range of 0.05-20 microg/mL with a mean correlation coefficient of around 0.996 for all analytes. The method was successfully applied to the analysis of the respective drugs in commercial formulations and in human urine without interference from other constituents. These data show that MCE has a great potential for reliable drug analysis.

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Pulsed-laser fluorescence detection in capillary zone electrophoresis of some banned substances in sport

Cited by 26 publications

References 14 publications

Recent progress in pharmacokinetic applications of capillary electrophoresis

Recent progress in pharmacokinetic applications of capillary electrophoresis

Indirect laser-induced fluorescence detection of diuretics separated by capillary electrophoresis

Fast quantitative determination of diuretic drugs in tablets and human urine by microchip electrophoresis with native fluorescence detection

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