Pupae protein extracts exert anticancer effects by downregulating the expression of IL-6, IL-1β and TNF-α through biomolecular changes in human breast cancer cells

Chukiatsiri, Suttida; Siriwong, Supatcharee; Thumanu, Kanjana

doi:10.1016/j.biopha.2020.110278

Cited by 24 publications

(15 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, cancer is mostly treated with radiotherapy and chemotherapy, which have side effects on the body, so finding natural antitumor drugs would be a better option. In vitro studies found the protein hydrolysates and amino acids in silkworm pupae had anticancer effects and were cytotoxic to human stomach cancer cells, breast cancer cells, and liver cancer cells [ 33 , 44 , 46 ]. Both silkworm pupa protein and silkworm pupa oil were found to have anticancer activity.…”

Section: Pharmacological Functions and Mechanisms Of Silkworm Pupaementioning

confidence: 99%

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Zhou

Duan

et al. 2022

Foods

View full text Add to dashboard Cite

Silkworm pupae are insects that are beneficial to human health, not only for their high nutritional value but, more importantly, for the variety of pharmacological functions they can perform when consumed. Currently, there is a lot of interest in the pharmaceutical applications of silkworm pupae. In recent years, the biological functions of domestic silkworm pupae have gradually been identified and confirmed, especially for their beneficial effects on human health. Studies have found that silkworm pupae have positive effects on liver protection, immune enhancement, antiapoptosis, antitumour, antibacterial, regulation of blood glucose and blood lipids, and lowering of blood pressure. However, the pharmacological mechanisms and systemic safety of silkworm pupae have not been systematically evaluated. In this paper, the nutritional composition of the pupae of the domestic silkworm is first summarised. The pharmacological functions of silkworm pupae and their components are then classified, and their mechanisms of occurrence are described. In addition, we provide a preliminary evaluation of the safety of silkworm pupae, analyse their application prospects, and suggest future directions for further pharmacological function studies. The aim is to generate interest in the promotion of human health through the use of silkworm pupae.

show abstract

Section: Pharmacological Functions and Mechanisms Of Silkworm Pupaementioning

confidence: 99%

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Zhou

Duan

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…GFAP is identified as a major intermediate filament protein in astrocytes 47 . IL‐6, IL‐1β and TNF‐α are pro‐inflammatory cytokines 48 . Here, we first discovered that MPTP injection into the mice increased the levels of IL‐6, IL‐1β, iNOS, COX‐2 and TNF‐α in nigra, whereas shikonin treatment restored the promotion in the levels of inflammatory cytokines and mediators.…”

Section: Discussionmentioning

confidence: 83%

“…47 IL-6, IL-1β and TNF-α are pro-inflammatory cytokines. 48 Here, we first discovered that MPTP injection into the mice increased the levels of IL-6, IL-1β, iNOS, COX-2 and TNF-α in nigra, whereas shikonin treatment restored the promotion in the levels of inflammatory cytokines and mediators. We found that MPTP injection contributed to an irregular triangular shape, whereas shikonin treatment alleviated the changes.…”

Section: Shikonin Naphthoquinone Pigment Serves As a Traditionalmentioning

confidence: 91%

Shikonin ameliorates oxidative stress and neuroinflammation via the Akt/ERK/JNK/NF‐κB signalling pathways in a model of Parkinson's disease

Guo

et al. 2022

Clin Exp Pharma Physio

View full text Add to dashboard Cite

Parkinson's disease (PD) is the second most common neurodegenerative disorder.Shikonin plays protective roles in age-associated diseases. Therefore, we investigate the biological functions of shikonin and its mechanisms involved in PD pathogenesis.The neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to mimic PD-like conditions in animal models. The learning and memory capacities were assessed by Morris water-maze test, pole test, locomotor activity test and rotarod test. Neuroinflammation was determined by measuring the levels of tumour necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). The quantification of superoxide dismutase, malondialdehyde and glutathione in substantia nigra was performed to estimate oxidative damage. Histopathologic changes were examined by haematoxylin and eosin staining. Immunofluorescence staining was conducted to determine the activation of astrocytes, tyrosine hydroxylase (TH)-positive neurons, and nuclear translocation of p65. Immunohistochemistry was performed to evaluate dopamine transporter (DAT)-positive neurons. Protein levels were measured by western blotting. Shikonin alleviates the cognitive and behavioural impairments. The death of dopaminergic neurons in nigra was attenuated by shikonin. The MPTP-induced neuroinflammation and oxidative stress in substantia nigra were alleviated by shikonin administration. Shikonin ameliorated the neuronal damage in nigra and inhibited the activation of astrocyte. Shikonin modulated the protein kinase B (Akt)/extracellular regulated kinase (ERK)/c-Jun N-terminal kinase (JNK)/nuclear factor κB (NF-κB) pathways. Shikonin ameliorates dopaminergic neuronal apoptosis by inhibiting oxidative stress and neuroinflammation via the Akt/ERK/JNK/NF-κB pathways in PD. The study has several limitations. First, in a previous study, levels of phosphorylated ERK were increased by MPTP. In our current study, we observed decreased p-ERK in nigra following MPTP treatment. Therefore, further investigation in the mechanisms of shikonin against PD progression is required. Second, the biological functions of shikonin need more exploration, including mitochondrial function and autophagy. Moreover, specific molecular targets for shikonin remain uncertain. Lei Guo and Yuanyuan Li contributed equally to this work.

show abstract

“…Not only the actions of inhibiting cell proliferation and promoting apoptosis, but also other mechanisms have been proposed to explain the anti-tumor effect of SPPHs, such as anti-inflammatory and antioxidant activities [ 7 , 8 ]. Some researchers have tried to find the specific morphological changes of the apoptosis process.…”

Section: Introductionmentioning

confidence: 99%

Effect of Silkworm Pupa Protein Hydrolysates on Proliferation of Gastric Cancer Cells In Vitro

Zou

et al. 2022

Foods

View full text Add to dashboard Cite

The proliferation inhibition effects of the hydrolysates from silkworm pupa proteins on MGC-803 gastric cancer cells were investigated in this study. The specific morphological changes (cell membrane, cell nucleus and cytoskeleton) of cells were measured. In vitro, the proliferation of MGC-803 cells was inhibited by silkworm pupa protein hydrolysates (SPPHs) in a dose-dependent manner. The flow cytometry analysis showed that the blocking effect of SPPHs on the MGC-803 cells was mainly in the G0/G1-phase. The morphological changes, disintegration of the cytoskeleton and retardant cell cycles were probably related to the activation of apoptosis. Thus, SPPHs could be promising as a chemopreventive agent due to their ability to promote apoptosis of tumor cells.

show abstract

Pupae protein extracts exert anticancer effects by downregulating the expression of IL-6, IL-1β and TNF-α through biomolecular changes in human breast cancer cells

Cited by 24 publications

References 47 publications

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Shikonin ameliorates oxidative stress and neuroinflammation via the Akt/ERK/JNK/NF‐κB signalling pathways in a model of Parkinson's disease

Effect of Silkworm Pupa Protein Hydrolysates on Proliferation of Gastric Cancer Cells In Vitro

Contact Info

Product

Resources

About