Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes

Walz, Alfred; Peveri, Paola; Aschauer, Harald; Baggiolini, Marco

doi:10.1016/0006-291x(87)90432-3

Cited by 470 publications

(229 citation statements)

References 12 publications

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“…3a). Moreover, a decrease of CXCR1 expression was detected after maturation induced by TNF-, IFN-and PGE 2 (Fig. 3a), consistent with a previous report showing that CXCR1 expression is downregulated in the presence of LPS.…”

Section: Dcs Are Responsive To Il-8supporting

confidence: 92%

Dendritic cells delivered inside human carcinomas are sequestered by interleukin‐8

Feijoó

Alfaro

Mazzolini

et al. 2005

Intl Journal of Cancer

111

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In the course of a clinical trial consisting of intratumoral injections of dendritic cells (DCs) transfected to produce interleukin-12, the use of In-labeled tracing doses of DCs showed that most DCs remained inside tumor tissue, instead of migrating out. In search for factors that could explain this retention, it was found that tumors from patients suffering hepatocellular carcinoma, colorectal or pancreatic cancer were producing IL-8 and that this chemokine attracted monocyte-derived dendritic cells that uniformly express both IL-8 receptors CXCR1 and CXCR2. Accordingly, neutralizing antihuman IL-8 monoclonal antibodies blocked the chemotactic attraction of DCs by recombinant IL-8, as well as by the serum of the patients or culture supernatants of human colorectal carcinomas. In addition, tissue culture supernatants of colon carcinoma cells inhibited DC migration induced by MIP-3b in an IL-8-dependent fashion. IL-8 production in malignant tissue and the responsiveness of DCs to IL-8 are a likely explanation of the clinical images, which suggest retention of DCs inside human malignant lesions. Impairment of DC migration toward lymphoid tissue could be involved in cancer immune evasion. ' 2005 Wiley-Liss, Inc.

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Section: Dcs Are Responsive To Il-8supporting

confidence: 92%

Dendritic cells delivered inside human carcinomas are sequestered by interleukin‐8

Feijoó

Alfaro

Mazzolini

et al. 2005

Intl Journal of Cancer

111

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“…The most likely explanation would be that NAP/IL-8 is the cytokine responsible for the effect seen in partially purified IL-1 preparations. NAP/IL-8 is known to elicit release of PMNL primary granule constituents like ß-glucuronidase, myeloperoxidase, or elastase in cytochalasin B-pretreated PMNL (13,15,24) .…”

Section: Resultsmentioning

confidence: 99%

“…Purified NAP/IL-8 shows potent chemotatic activity for human neutrophils in vitro (13,14,16,17), activates the release ofsuperoxide anions (13,24), and elicits release ofthe primary granule constituents from neutrophils (myeloperoxidase, /3-glucuronidase, and elastase) (13,15,24) .…”

mentioning

confidence: 99%

The monocyte-derived neutrophil activating peptide (NAP/interleukin 8) stimulates human neutrophil arachidonate-5-lipoxygenase, but not the release of cellular arachidonate.

Schröder

1989

The Journal of Experimental Medicine

162

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LPS and mitogen-stimulated mononuclear cells secrete a cytokine, which is able to activate the PMNL-arachidonate-5-lipoxygenase. This cytokine has been proven to be identical with the recently characterized novel neutrophil-activating peptide NAP/IL-8. NAP/IL-8 is able to activate human PMNL for release of LTB4, omega-oxidized LTB4, and 5-HETE in the presence of exogenous AA. Half-maximal concentration of NAP/IL-8 for release of LTB4 has been found to be near 4 x 10(-8) mol/liter. Time course studies revealed rapid activation of PMNL, with maximal release of LTB4 within the first 10 min with a decline up to 40 min. High amounts of omega-oxidized LTB4 were detected up to that time. Significant amounts of AA-5-LO-products can be detected only when PMNL were stimulated with NAP/IL-8 in the presence of exogenous AA. The concentration of AA necessary for half-maximal LTB4 release has been found to be 3 x 10(-6) mol/liter. In the presence of 8 x 10(-9) mol/liter [3H]AA, NAP/IL-8 (10(-9) to 10(-7) mol/liter) did not induce the production of LTB4, omega-oxidized LTB4, or 5-HETE. In addition, PMNL prelabeled with [3H]AA did not release either [3H]AA or 5-lipoxygenase metabolites when stimulated with NAP/IL-8 (10(-9) to 10(-7) mol/liter), indicating that NAP/IL-8 apparently does not activate cellular phospholipases/diacylglycerol-lipases. Apart from FMLP, C5a, and PAF NAP/IL-8 is the fourth clearly characterized neutrophil chemotaxin able to activate the PMNL-5-lipoxygenase. The detection of large amounts of NAP/IL-8, arachidonic acid, as well as LTB4-like material, in lesional material of patients with psoriasis points towards a possibly important role of NAP/IL-8 in amplifying inflammatory processes by induction of LTB4-production.

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“…Recruitment of these neutrophils is dependent on local production of chemotactic factors at the site of inflammation. One of the chemotactic factors which may be responsible for the attraction of neutrophils is IL-8 [1], also known as monocyte-driven neutrophil chemotactic factor (MDNCF) [2] or neutrophil-activating factor (NAF) [3]. IL-8 is produced by various cell types, including monocytes but also by other lymphoid cells, endothelial cells, keratinocytes, epithelial cells and various tumour cell lines [1,4,5], and its synthesis is induced by stimuli such as lipopolysaccharide (LPS), tumour necrosis factor-alpha (TNF-) and IL-1 .…”

Section: Introductionmentioning

confidence: 99%

Regulation and production of IL-8 by human proximal tubular epithelial cells in vitro

Gerritsma¹,

Hiemstra

Gerritsen³

et al. 1996

Clinical and Experimental Immunology

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SUMMARYA number of inflammatory kidney diseases are associated with interstitial nephritis and influx of leucocytes in the renal interstitium. Potentially the influx of neutrophils in the interstitium may be induced by the chemotactic cytokine IL-8. In the present study we have analysed the production of IL-8 by cultured human proximal tubular epithelial cells (PTEC) in response to a number of proinflammatory cytokines. Primary cell lines of proximal tubular epithelium obtained from ten different kidneys, and cultured under serum-free conditions, were found to produce IL-8 to different degrees from not detectable levels up to 10

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Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes

Cited by 470 publications

References 12 publications

Dendritic cells delivered inside human carcinomas are sequestered by interleukin‐8

Dendritic cells delivered inside human carcinomas are sequestered by interleukin‐8

The monocyte-derived neutrophil activating peptide (NAP/interleukin 8) stimulates human neutrophil arachidonate-5-lipoxygenase, but not the release of cellular arachidonate.

Regulation and production of IL-8 by human proximal tubular epithelial cells in vitro

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