Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein

Kumar, N. S. Sampath; Nazeer, R. A.; Jaiganesh, R.

doi:10.1016/j.peptides.2011.05.020

Cited by 122 publications

(43 citation statements)

References 34 publications

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“…In addition, NNAEYYK had two Tyr, which could effectively increase its antioxidant activity. Previous studies show that Cys is hydrophobic in nature and can interact directly with free radicals by donating the sulfur hydrogen, so the presence of Cys is one of the reasons for the good antioxidant activity of the isolated peptide [25]. Li et al considered that Cys residue at the C -terminus or next to the C -terminus plays an important role in antioxidative activities [26].…”

Section: Resultsmentioning

confidence: 99%

Purification of Antioxidant Peptides by High Resolution Mass Spectrometry from Simulated Gastrointestinal Digestion Hydrolysates of Alaska Pollock (Theragra chalcogramma) Skin Collagen

Sun

Chang

et al. 2016

Marine Drugs

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In this study, the stable collagen hydrolysate was prepared by alcalase hydrolysis and twice simulated gastrointestinal digestion from Alaska pollock skin. The characteristics of hydrolysates and antioxidant activities in vitro, including 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS•+) scavenging activity, ferric-reducing antioxidant power (FRAP) and hydroxyl radical (OH·) scavenging activity, were determined. After twice simulated gastrointestinal digestion of skin collagen (SGI-2), the degree of hydrolysis (DH) reached 26.17%. The main molecular weight fractions of SGI-2 were 1026.26 and 640.53 Da, accounting for 59.49% and 18.34%, respectively. Amino acid composition analysis showed that SGI-2 had high content of total hydrophobic amino acid (307.98/1000). With the simulated gastrointestinal digestion progressing, the antioxidant activities increased significantly (p < 0.05). SGI-2 was further purified by gel filtration chromatography, ion exchange chromatography and high performance liquid chromatography, and the A1a3c–p fraction with high hydroxyl radical scavenging activity (IC50 = 7.63 μg/mL) was obtained. The molecular weights and amino acid sequences of key peptides of A1a3c–p were analyzed using high resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-MS) combined with de novo software and UniProt of MaxQuant software. Four peptides were identified from A1a3c–p, including YGCC (444.1137 Da) and DSSCSG (554.1642 Da) identified by de novo software and NNAEYYK (900.3978 Da) and PAGNVR (612.3344 Da) identified by UniProt of MaxQuant software. The molecular weights and amino acid sequences of four peptides were in accordance with the features of antioxidant peptides. The results indicated that different peptides were identified by different data analysis software according to spectrometry mass data. Considering the complexity of LC-ESI-LTQ-Orbitrap-MS, it was necessary to use the different methods to identify the key peptides from protein hydrolysates.

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Section: Resultsmentioning

confidence: 99%

Purification of Antioxidant Peptides by High Resolution Mass Spectrometry from Simulated Gastrointestinal Digestion Hydrolysates of Alaska Pollock (Theragra chalcogramma) Skin Collagen

Sun

Chang

et al. 2016

Marine Drugs

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“…Lots of researches have indicated that antioxidant peptides with cysteine display significant free radicals scavenging activity. Sampath et al used gastrointestinal digestive enzymes to hydrolyze the horse mackerel (Magalaspis cordyla) viscera protein and isolated a peptide (Ala-Cys-Phe-Leu) with strong activity against DPPH and hydroxyl radical (89.2±1.7 and 59.1±1.2 %) [45]. Ngo et al identified a peptide (Thr-Cys-Ser-Pro) from the papain hydrolysate of the Pacific cod (Gadus macrocephalus) [46].…”

Section: The Composition Of Amino Acidsmentioning

confidence: 99%

“…Commercial proteases, such as papain [54], neutrase [55], alcalase [56], flavourzyme [57], and gastrointestinal digestive enzymes (pepsin, trypsin, and α-chymotrypsin) [45] are widely used in hydrolyzing proteins. Some unidentified proteases or crude enzyme from microorganism were occasionally reported to be useful in releasing potential antioxidant peptides, such as crude enzyme preparation from Aspergillus clavatus ES1 hydrolyzing protein of Sardinelle (S. aurita) in Ali Bougatef 's study [39].…”

Section: Isolation and Purification Of Antioxidant Peptidesmentioning

confidence: 99%

Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods

Wü

Liú

et al. 2015

Appl Biochem Biotechnol

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Marine organisms are rich sources of structurally diverse bioactive nitrogenous components. In recent years, numerous bioactive peptides have been identified in a range of marine protein resources, such as antioxidant peptides. Many studies have approved that marine antioxidant peptides have a positive effect on human health and the food industry. Antioxidant activity of peptides can be attributed to free radicals scavenging, inhibition of lipid peroxidation, and metal ion chelating. Moreover, it has also been verified that peptide structure and its amino acid sequence can mainly affect its antioxidant properties. The aim of this review is to summarize kinds of antioxidant peptides from various marine resources. Additionally, the relationship between structure and antioxidant activities of peptides is discussed in this paper. Finally, current technologies used in the preparation, purification, and evaluation of marine-derived antioxidant peptides are also reviewed.

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“…Even though, antioxidant activity has been reported in masou salmon (Oncorhynchus masou) (Dh et al, 2008), croaker (Otolithes ruber) (Nazeer et al, 2012), sardinelle (Sardinella aurita) (Bougatef et al, 2010), tilapia (Oreochromis niloticus) (Zhang et al, 2012); among other species, only one study was found where horse mackerel (Magalaspis cordyla) viscera extracts had antioxidant activity (Sampath-Kumar et al, 2011). In addition, is worth to mention that antioxidant activity values reached by BF (1354 mmol Trolox/Kg) and ACF (1522 mmol Trolox/Kgby the DPPH method, are similar to those reported in fruits such as apple (1200-1600 mmol Trolox/Kg) and tangerine (1500-2000 mmol Trolox/Kg) (Pyo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Bioactive fractions from cantabrian anchovy (Engraulis encrarischolus) viscera

et al. 2016

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The potential of cantabrian anchovy (Engraulis encrarischolus) viscera as a source of bioactive compounds is of interest for both, pharmaceutical and food industries. Cantabrian anchovy guts and heads were freeze-dried, extracted with methanol and subjected to fractionation by solvent partitioning using hexane, ethyl acetate, and butanol. Fractions were tested for antimutagenic, antioxidant, antifungal, and antibacterial activity using the Ames test; DPPH, ABTS, and FRAP assays; the radial grown inhibition assay; and the microbroth dilution method, respectively. Five fractions were obtained from the anchovy gut methanolic extract, in addition to the hexane-(HF), ethyl acetate-(EAF), and butanol-soluble (BF) fractions, an aqueous-soluble fraction (ALF) and precipitated crystals (ACF) in this were also obtained. HF and EAF resulted to be antimutagenic, HF and ALF showed antifungal activity, BF and ACF showed the highest antioxidant potential, and HF and BF were antibacterial against several strains. Anchovy gut, which to the present study had not been reported for any bioactivity, has antimutagenic, antifungal, antioxidant, and antibacterial compounds, which need to be isolated for full characterization and study.

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Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein

Cited by 122 publications

References 34 publications

Purification of Antioxidant Peptides by High Resolution Mass Spectrometry from Simulated Gastrointestinal Digestion Hydrolysates of Alaska Pollock (Theragra chalcogramma) Skin Collagen

Purification of Antioxidant Peptides by High Resolution Mass Spectrometry from Simulated Gastrointestinal Digestion Hydrolysates of Alaska Pollock (Theragra chalcogramma) Skin Collagen

Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods

Bioactive fractions from cantabrian anchovy (Engraulis encrarischolus) viscera

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