Purification and characterization of a novel serine protease from the mushroom Pholiota nameko

Guan, G.P.; Zhang, Guoqing; Wu, Ying‐Ying; Wang, Hexiang; Ng, T.B.

doi:10.1016/j.jbiosc.2011.02.009

Cited by 13 publications

(6 citation statements)

References 31 publications

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“…Absorbance of supernatant was measured at 280 nm using a UV-spectrophotometer (TU-1810, PERSEE, Beijing, China). One unit (U) of protease activity was defined as a 0.001 absorbance increase per minute in supernatant per milliliter of reaction mixture [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

High temperature enhances the ability of Trichoderma asperellum to infect Pleurotus ostreatus mycelia

Qiu

Zhang

et al. 2017

PLoS ONE

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Trichoderma asperellum is one of the species which can be isolated from contaminated Pleurotus ostreatus cultivation substrate with green mold disease. This study focused on the relationship between high temperature and infectivity of T. asperellum to P. ostreatus. Antagonism experiments between T. asperellum and P. ostreatus mycelia revealed that high temperature-treated P. ostreatus mycelia were more easily infected by T. asperellum and covered by conidia. Microscopic observation also showed that P. ostreatus mycelia treated with high temperature could adsorb more T. asperellum conidia. Furthermore, conidia obtained from T. asperellum mycelia grown at 36°C featured higher germination rate compared with that incubated at 28°C. High temperature-treated T. asperellum mycelia can produce conidia in shorter periods, and T. asperellum mycelia were less sensitive to high temperature than P. ostreatus. Deactivated P. ostreatus mycelia can induce T. asperellum cell wall-degrading enzymes (CWDEs) and P. ostreatus mycelia subjected to high temperature showed induced CWDEs more effective than those incubated at 28°C. Moreover, T. asperellum showed higher CWDEs activity at high temperature. In dual cultures, hydrogen peroxide (H2O2) increased after 36°C, and high concentration of H2O2 could significantly inhibit the growth of P. ostreatus mycelia. In summary, our findings indicated for the first time that high temperature can induce a series of mechanisms to enhance infection abilities of T. asperellum to P. ostreatus mycelia and to cause Pleurotus green mold disease.

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Section: Methodsmentioning

confidence: 99%

High temperature enhances the ability of Trichoderma asperellum to infect Pleurotus ostreatus mycelia

Qiu

Zhang

et al. 2017

PLoS ONE

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“…O valor de temperatura ótima encontrado no presente estudo está de acordo com aqueles reportados para outros fungos filamentos. Guan et al (2011) et al, 2011). A velocidade de reação aumenta à medida que a temperatura inicialmente aumenta, devido à elevação da energia cinética da reação de moléculas.…”

Section: Efeito Do Ph E Da Temperatura Na Atividade E Na Estabilidade...unclassified

Proteases ácidas de cogumelo comestível da Amazônia para aplicabilidade industrial

et al. 2017

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As proteases constituem um grupo de enzimas que apresentam elevada aplicabilidade em diversos setores industriais, sendo obtidas de fontes animais, vegetais e microbianas. Entre os fungos, os cogumelos têm se destacado como fonte de proteases com potencial biotecnológico. O objetivo deste trabalho foi investigar a produção de proteases por cogumelos, utilizando a fermentação em meio líquido. A cultura matriz foi preparada em ágar batata dextrose, acrescida de extrato de levedura 0,5% (p/v), e o bioprocesso foi conduzido durante cinco dias, a 150 rpm e 25 °C. Azocaseína 1% (p/v) foi utilizada como substrato para avaliar a atividade proteolítica dos cogumelos. Entre as espécies avaliadas, Pleurotus albidus (Berk.) Pegler 1983 foi a produtora significativa de proteases (34,00 U/mL) e a síntese dessas enzimas foi estimulada pela idade e pelo volume do inóculo. Essas enzimas mostraram atividade ótima em pH 5 a 60 °C e estabilidade em pH 5-8, com temperaturas de 30 a 60 °C. As proteases foram classificadas como cisteíno e serinoproteases.

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“…At high temperatures, the enzymes suffer protein denaturation because the intramolecular bonds are affected (Ahmed et al, 2011). In the studies of Fonseca et al (2014), Guan et al (2011) and Machado et al (2016), the optimum temperature activity of the mushrooms P. ostreatoroseus, Pholiota nameko and L. citrinus was also determined at 40 and 50°C, respectively. The stability of pH and temperature is an important parameter in the enzymes application due to their determination of economic availability in the industrial processes (Moretti et al, 2012).…”

Section: Effect Of Ph and Temperature On Enzyme Activity And Stabilitymentioning

confidence: 99%

Production and characterization of proteases from edible mushrooms cultivated on amazonic tubers

Machado¹,

Martim²,

Alecrim³

et al. 2017

Afr. J. Biotechnol.

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Proteases are important commercial enzymes, and among their numerous sources are the Basidiomycetes. The use of proteases in many industrial areas promotes the search for enzymes with new properties. The aim of this study is to produce and characterize peptidases of a biocomposite from mycelial biomass grown in Amazonic tubers. Lentinus citrinus DPUA 1535 and Pleurotus ostreatoroseus DPUA 1720 were cultivated on Dioscorea trifida, Manihot esculenta and Dioscorea alata supplemented with rice bran or manioc flour residue in different proportions. The highest proteolytic activity was determined in the crude extract from P. ostreatoroseus grown in D. alata (DA) without supplementation (142.22 U/mL). The enzymes showed optimum activities at 40°C and pH 7.0; and stability at 50°C and pH 8.0. The proteases were classified as cysteine proteases based on the effect of inhibitors used.

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Purification and characterization of a novel serine protease from the mushroom Pholiota nameko

Cited by 13 publications

References 31 publications

High temperature enhances the ability of Trichoderma asperellum to infect Pleurotus ostreatus mycelia

High temperature enhances the ability of Trichoderma asperellum to infect Pleurotus ostreatus mycelia

Proteases ácidas de cogumelo comestível da Amazônia para aplicabilidade industrial

Production and characterization of proteases from edible mushrooms cultivated on amazonic tubers

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