Purification and characterization of an insulin-related peptide in the desert locust, Schistocerca gregaria: immunolocalization, cDNA cloning, transcript profiling and interaction with neuroparsin

Badisco, Liesbeth; Claeys, Ilse; Hiel, Matthias B. Van; Clynen, Elke; Huybrechts, Jurgen; Vandersmissen, Tim; Soest, Sofie Van; Bosch, Luc Vanden; Simonet, Gert; Broeck, Jozef Vanden

doi:10.1677/jme-07-0161

Cited by 65 publications

(62 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with the findings in desert locusts, Schistocerca gregaria (Badisco et al, 2008). The highest expression levels occurred in the ventriculus and fat body of reproducing queens and males.…”

Section: Resultssupporting

confidence: 93%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

View full text Add to dashboard Cite

Understanding the social evolution leading to insect eusociality requires, among other, a detailed insight into endocrine regulatory mechanisms that have been co-opted from solitary ancestors to play new roles in the complex life histories of eusocial species. Bumblebees represent well-suited models of a relatively primitive social organization standing on the mid-way to highly advanced eusociality and their queens undergo both, a solitary and a social phase, separated by winter diapause. In the present paper, we characterize the gene expression levels of major endocrine regulatory pathways across tissues, sexes, and life-stages of the buff-tailed bumblebee, Bombus terrestris, with special emphasis on critical stages of the queen's transition from solitary to social life. We focused on fundamental genes of three pathways: (1) Forkhead box protein O and insulin/insulin-like signaling, (2) Juvenile hormone (JH) signaling, and (3) Adipokinetic hormone signaling. Virgin queens were distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and JH signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1). Diapausing queens showed the expected downregulation of JH signaling in terms of low MFE and vitellogenin (Vg) expressions, but an unexpectedly high expression of Kr-h1. By contrast, reproducing queens revealed an upregulation of MFE and Vg together with insulin signaling. Surprisingly, the insulin growth factor 1 (IGF-1) turned out to be a queen-specific hormone. Workers exhibited an expression pattern of MFE and Vg similar to that of reproducing queens. Males were characterized by high Kr-h1 expression and low Vg level. The tissue comparison unveiled an unexpected resemblance between the fat body and hypopharyngeal glands across all investigated genes, sexes, and life stages.

show abstract

Section: Resultssupporting

confidence: 93%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…This suggests the lack of processing enzymes to generate mature insulin in the fat body, which probably explains why fat body-derived ILPs in different species have attained similar structural features as IGFLPs (shortened C-peptide and/or the loss of cleavage sites) despite their independent lineages. Studies in orthopteran species (which are considered closer to earlier insect species), where there is only one identified ILP the expression of which is differentially regulated in the brain IPCs and in the fat body (Kromer-Metzger and Lagueux, 1994; Badisco et al, 2008), support our hypothesis.…”

Section: Discussionsupporting

confidence: 78%

A Fat Body-Derived IGF-like Peptide Regulates Postfeeding Growth in Drosophila

et al. 2009

View full text Add to dashboard Cite

SUMMARY Members of the insulin family of peptides have conserved roles in the regulation of growth and metabolism in a wide variety of metazoans. Here we show that Drosophila insulin-like peptide 6 (DILP6), which is structurally similar to vertebrate insulin-like growth factor (IGF), is predominantly expressed in the fat body, a functional equivalent of the vertebrate liver and adipocytes. This expression occurs during the postfeeding stage under the direct regulation of ecdysteroid. We further reveal that dilp6 mutants show growth defects during the postfeeding stage, which results in reduced adult body size through a decrease in cell number. This phenotype is rescued by fat body-specific expression of dilp6. These data indicate that DILP6 is a functional, as well as a structural, counterpart of vertebrate IGFs. Our data provide in vivo evidence for a role of ILPs in determining adult body size through the regulation of postfeeding growth.

show abstract

“…Sequence analysis indicates that mosquito OEH is a member of the neuroparsin family of insect neuropeptides, which share some homology with mammalian insulin growth factor binding proteins. This interaction was demonstrated for a neuroparsin and ILP from Locusta migratoria (Badisco et al, 2008), but its functional significance in this insect is not resolved. The primary gonadotropic activity of OEH in two related mosquito species combined with our lack of knowledge of how OEH and other neuroparsins function led us to examine whether OEH activity depends upon the IR.…”

Section: Discussionmentioning

confidence: 97%

Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito, Georgecraigius atropalpus, after adult eclosion or a blood meal

Gulia-Nuss

Eum

Strand

et al. 2012

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYThe rockpool mosquito, Georgecraigius atropalpus, is a facultatively autogenous species that produces its first egg clutch without a blood meal shortly after emergence. Several days after depositing this clutch, females must take a blood meal to produce a second egg clutch. Decapitation of females shortly after emergence or blood ingestion prevents egg maturation. Here, we report that a single injected dose of the neuropeptide ovary ecdysteroidogenic hormone (OEH) fully restored egg maturation in decapitated females in both circumstances. This neuropeptide and two insulin-like peptides (ILPs) are potent gonadotropins in the related yellow fever mosquito, Aedes aegypti. ILP3 was marginally restorative in decapitated G. atropalpus, and ILP4 had no effect. Egg maturation in non-and blood-fed G. atropalpus was dependent on the enzymatic mobilization of amino acids from stored protein or the blood meal for yolk protein (vitellogenin, VG) synthesis and uptake by oocytes. We further show that OEH stimulates serine protease activity in the fat body of newly eclosed females or in the midgut of blood-fed ones, and ecdysteroid hormone production by the ovaries of both females. In contrast, only 20-hydroxyecdysone stimulated VG synthesis in the fat body of non-and blood-fed females. Using RNA interference to knock down expression of the insulin receptor, we found that OEH still fully restored autogenous egg maturation. In summary, our results identify OEH as a primary regulator of egg maturation in both autogenous and blood-fed G. atropalpus females and suggest the shift from blood meal-dependent to blood meal-independent release of OEH is a key factor in the evolution of autogeny in this species.

show abstract

Purification and characterization of an insulin-related peptide in the desert locust, Schistocerca gregaria: immunolocalization, cDNA cloning, transcript profiling and interaction with neuroparsin

Cited by 65 publications

References 48 publications

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

A Fat Body-Derived IGF-like Peptide Regulates Postfeeding Growth in Drosophila

Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito, Georgecraigius atropalpus, after adult eclosion or a blood meal

Contact Info

Product

Resources

About