Purification and characterization of antifungal phenazines from a fluorescent Pseudomonas strain FPO4 against medically important fungi

Gorantla, Jaggaiah N.; Kumar, Sanjay; Nisha, G.V.; Sumandu, A.S.; Dileep, C. S.; Sudaresan, A.; Kumar, Manoj; Lankalapalli, Ravi S.; Kumar, B. Santhosh

doi:10.1016/j.mycmed.2014.02.003

Cited by 25 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phenazine‐1‐carboxylic acid (PCA) is a nitrogen‐containing heterocyclic molecule with profound antifungal activity and is used in both agriculture and medicine . Among the PCA‐producing bacterial genera, Pseudomonas and Streptomyces have been extensively studied as biological control agents .…”

Section: Introductionmentioning

confidence: 99%

“…Phenazine-1-carboxylic acid (PCA) is a nitrogen-containing heterocyclic molecule with profound antifungal activity and is used in both agriculture and medicine. 1,2 Among the PCA-producing bacterial genera, Pseudomonas and Streptomyces have been extensively studied as biological control agents. 3,4 PCA exerts a curative effect on many crop fungal diseases, including cucumber anthracnose, ginger rhizome rot, potato scab, pepper Phytophthora blight and wheat take-all disease.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adsorption/desorption characteristics, separation and purification of phenazine‐1‐carboxylic acid from fermentation extract by macroporous adsorbing resins

Bilal

Yue

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND Pseudomonas chlororaphis GP72 is a biocontrol strain with great capacity to produce a wide range of secondary metabolites including phenazine derivatives. Phenazine‐1‐carboxylic acid (PCA) is an environmentally friendly antibiotic with broad‐spectrum biological and pharmaceutical activities. Many studies have been focused on engineering high‐yielding strains and optimizing fermentation bioprocesses to enhance PCA biosynthesis. However, the separation and purification of PCA in biotechnological production remain to be addressed. In this context, the present work intended to develop a simple and proficient technique for the separation and purification of PCA from the fermentation broth of engineered P. chlororaphis GP72AN. RESULTS Five macroporous adsorbents with different physicochemical characteristics were screened to present their PCA separation potential. Among them, HP‐20 resin was found highly suitable based on its promising adsorption and desorption performance in a static batch process. Langmuir and Freundlich isotherms deciphered the solute binding affinity towards HP‐20 adsorbent at varying temperatures, and the experimental data fitted best to the Langmuir isotherm model. The column‐assisted dynamic adsorption–desorption trials were executed to optimize the functional parameters for PCA separation. Under standardized separating conditions, the average adsorption capacity and desorption ratio of PCA were 24.4 ± 1.5 mg g−1 dry resin and 86.5 ± 1.3%, respectively. After treatment with HP‐20 resin, PCA contents in the product were considerably increased with a recovery yield of 78.4 ± 2.4%. CONCLUSION In conclusion, the method proposed herein achieved effective separation and purification of PCA by applying HP‐20 resin. The results obtained also suggest an encouraging basis for scale‐up preparation of PCA and other high‐value phenazine compounds of industrial interests. © 2018 Society of Chemical Industry

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adsorption/desorption characteristics, separation and purification of phenazine‐1‐carboxylic acid from fermentation extract by macroporous adsorbing resins

Bilal

Yue

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…Carboxylic acids have been shown to have antimicrobial properties [ 54 ], with significant antifungal activity against C. albicans [ 55 ]. However, Queiroz et al [ 45 ] demonstrated, in a test with a carboxylic acid isolated from C. heliotropiifolius , failed to show activity against C. albicans .…”

Section: Discussionmentioning

confidence: 99%

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

et al. 2021

View full text Add to dashboard Cite

Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic, and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal activity of the extract against opportunistic Candida strains was investigated using the microdilution method. The minimal fungicidal concentration (MFC) was determined by subculture, while the modulation of the morphological transition (fungal virulence) was evaluated by light microscopy. The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar phenolic compounds with known antioxidant action washighlighted. While the extract showed clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition to inhibiting the morphological transition associated with increased virulence in Candida strains. Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and antioxidant potential.

show abstract

“…were recognized as biocontrol and plant growth promoters (3,4). Documents have emphasized several antibiotics developed by the strains of Pseudomonas such as phenazine (5,6), 2,4-diacetylphloroglucinol (7), pyrrolnitrin (8), pyoluteorin (9), and siderophore (10) and their main functions are to control a variety of plant infection-causing fungi (11).…”

Section: Introductionmentioning

confidence: 99%

Elucidation and Identification of an Antifungal Compound from Pseudomonas aeruginosa DA3.1 Isolated from Soil in Vietnam

Trung

Cuong

Thảo

et al. 2020

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Fusarium sp. and Rhizoctonia sp. fungi have been always threats to short-term crops. In Vietnam, corn and soybean suffer serious losses annually. Therefore, it is necessary to utilize an environmentally friendly antifungal compound that is highly effective against phytopathogenic fungi. Pseudomonas sp. is a popular soil bacterial strain and well known for its high antifungal activity. Objectives: This study was carried out to evaluate and assess the antifungal activity of a local bacterial strain namely DA3.1 that was later identified as Pseudomonas aeruginosa. This would be strong scientific evidence to develop an environmentally friendly biocide from a local microorganism strain for commercial use. Methods: The antifungal compound was purified from ethyl acetate extraction of deproteinized cell culture broth by a silica gel column (CH2Cl2/MeOH (0% - 10% MeOH)). The purity of the isolated compound was determined by HPLC, and its molecular structure was elucidated using spectroscopic experiments including one-dimensional (1D) (1H NMR, 13C NMR, DEPT) and two-dimensional (2D) (HMBC and HSQC) spectra. The activity of the purified compound against Fusarium sp. and Rhizoctonia sp. fungi was measured using the PDA-disk diffusion method, and its growth-promoting ability was evaluated using the seed germination test of corn and soybean. Results: The results showed that the antifungal compound produced by Pseudomonas aeruginosa DA3.1 had a retention factor (Rf) of 0.86 on thin layer chromatography (TLC). Based on the evidence of spectral data including proton nuclear magnetic resonance (1H NMR), carbon nuclear magnetic resonance (13C NMR), distortionless enhancement by polarization transfer (DEPT), heteronuclear multiple bond correlation (HMBC), and heteronuclear single quantum coherence (HSQC), the chemical structure was elucidated as phenazine-1-carboxylic. The purified compound showed inhibitory activity against F. oxysporum and R. solani and exhibited the ability of the germination of corn and soybean seeds. The results revealed the benefit of native P. aeruginosa DA3.1 and phenazine-1-carboxylic acid for use as a biocontrol agent, as well as a plant growth promoter. Conclusions: The antifungal compound isolated from local Pseudomonas DA3.1 was identified as phenazine-1-carboxylic acid that posed high antifungal activity and was a plant germination booster.

show abstract

Purification and characterization of antifungal phenazines from a fluorescent Pseudomonas strain FPO4 against medically important fungi

Cited by 25 publications

References 26 publications

Adsorption/desorption characteristics, separation and purification of phenazine‐1‐carboxylic acid from fermentation extract by macroporous adsorbing resins

Adsorption/desorption characteristics, separation and purification of phenazine‐1‐carboxylic acid from fermentation extract by macroporous adsorbing resins

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

Elucidation and Identification of an Antifungal Compound from Pseudomonas aeruginosa DA3.1 Isolated from Soil in Vietnam

Contact Info

Product

Resources

About