Purification and characterization of bromelain from pineapple (Ananas comosus L.) peel waste

Abstract

Bromelain is widely used in food industry and pharmaceutical products due to its strong antioxidant properties. Therefore, the extraction of bromelain from pineapple peel may improve the profitability and sustainability of pineapple industry. The aim of this work is to show the purification, stability, and kinetics of bromelain from pineapple peel. By studying the stability of purified bromelain (PB), we found that the activity of PB was inhibited by Fe3+, Al3+, methanol, ethanol, and n‐butyl alcohol, while it was increased in the presence of Ca2+, ethylenediamine tetra acetic acid, glucose, D‐xylose, maltose, potassium sodium tartrate, sodium citrate, citric acid, and sodium nitrite. These stability tests will expand the application and space acquisition of bromelain. The kinetics study indicated that the thermal inactivation of PB was conforming to the first‐order reaction and the half‐life (t1/2) of PB under different temperature conditions (45, 55, 65, and 75 °C) was 81.54, 31.12, 10.28, and 5.23 min, respectively. Therefore, the inactivation time of PB can be predicted at different temperatures for food heating processing.Practical ApplicationThe potential of utilizing pineapple peel for bromelain extraction might improve the profitability and sustainability of the pineapple industry.

Beneficial Properties of Bromelain

“…Acquiring bromelain from this part of the pineapple is much more profitable because enzyme concentration in the pineapple stems is higher than in the fruit part. Moreover, the complex process of extraction and purification of SBM is cheaper [6,18] The molecular weight range for SBM is 26-37 kDa and for FBM the molecular weight range is 24.5-32 kDa [7,[18][19][20]. Significant data on biochemical analysis of the SBM extract is provided by Herrach et al 1995 [21].…”

“…In recent years, commercial production of bromelain extracts has increased significantly, which has contributed to the development of faster and more effective large-scale enzyme production and purification techniques. Currently, dietary supplements containing bromelain are used in many health currents [6,7].…”

Recent Advances and Insights into Bromelain Processing, Pharmacokinetics and Therapeutic Uses

“…Bromelain is one of the most thoroughly investigated proteolytic complexes that is characterized by several thiol endopeptidases [136]. As discussed in this review, bromelain is extracted predominantly from pineapple stems and fruit [53].…”

Pertumbuhan Kedelai pada Kondisi Cekaman Salinitas dengan Pemberian Ekstrak Kulit Buah Nanas (Ananas comosus L)

“…Dalam kulit buah nanas terdapat senyawa fitokimia, seperti flavonoid sebagai antioksidan potensial yang mampu melindungi sel dari kerusakan oksidatif (Hossain & Rahman 2011), senyawa polifenol yang juga sebagai antioksidan (Li et al 2014). Selain itu juga mengandung bromelain, sejenis enzim protease yang termasuk antioksidan kuat (Zhou et al 2021). Dengan berbagai fitokimia yang bersifat antioksidan tersebut, maka dimungkinkan pemberian ekstrak kulit buah nanas mampu melindungi sel klorofil dari potensi kerusakan.…”

“…(Suryaman et al 2021). Ekstrak kulit buah nanas mengandung senyawa polifenol yang termasuk antioksidan kuat, serta sebagai sumber enzim proteolitik seperti bromelain (Dorta & Sogi 2016) yang juga termasuk kategori antioksidan kuat (Zhou et al 2021). Selain mengandung fitokimia yang bersifat antioksidan, dalam ekstrak tersebut juga terdapat senyawa yang bersifat antibakteri.…”