PURIFICATION AND CHARACTERIZATION OF CURCIN, A TOXIC LECTIN FROM THE SEED OF<i>JATROPHA CURCAS</i>

Lin, Juan; Zhou, Xin; Wang, Jinya; Pin, Jiang; Tang, Kexuan

doi:10.1080/10826060903558588

Cited by 44 publications

(27 citation statements)

References 22 publications

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“…The glycosylation, molecular weight, pI, and sugar content data are in accordance with the values reported by Stirpe 29) for RIPs and Lin et al 30) for curcin.…”

Section: Resultssupporting

confidence: 76%

Cytotoxic effect of the immunotoxin constructed of the ribosome-inactivating protein curcin and the monoclonal antibody against Her2 receptor on tumor cells

Jaramillo-Quintero

Rojas

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…The glycosylation, molecular weight, pI, and sugar content data are in accordance with the values reported by Stirpe 29) for RIPs and Lin et al 30) for curcin.…”

Section: Resultssupporting

confidence: 76%

Cytotoxic effect of the immunotoxin constructed of the ribosome-inactivating protein curcin and the monoclonal antibody against Her2 receptor on tumor cells

Jaramillo-Quintero

Rojas

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…3B). Purified hemagglutinin from J. curcas seeds had a pI value of 5.75 (Cano-Asseleih et al, 1989), while in curcin, the pI value was 8.54 (Juan Lin et al, 2010).…”

Section: Ief Of Protein Fractions From J Curcas Seedsmentioning

confidence: 99%

“…The albumin 2S (~ 12 kDa) was identified as the first known allergen from J. curcas (Menezes et al, 2009). J. curcas also contains a toxic protein, named curcin, with a molecular weight of ~ 28.2 kDa (Juan Lin et al, 2010) similar to that found in albumin and prolamin fractions.…”

Section: Electrophoresis Of Protein Fractions From J Curcas Defattedmentioning

confidence: 99%

Biochemical evaluation of protein fractions from physic nut (<i>Jatropha curcas</i> L.)

et al. 2012

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RESUMEN Evaluación bioquímica de fracciones proteínicas de piñón Mexicano (Jatropha curcas L.).Las proteínas de semillas de J. curcas L. se fraccionaron empleando el método de Osborne y posteriormente se determinaron algunas de sus propiedades bioquímicas y nutricionales. Las fracciones mayoritarias resultaron ser glutelinas (378 g kg -1 de proteína) y globulinas (201 g kg -1de proteína) mientras que las albúminas y prolaminas fueron las fracciones minoritarias. La digestibilidad de la proteína resultó ser más alta en las glutelinas y globulinas, con valores de 81 y 80% respectivamente. El análisis por electroforesis mostró que las globulinas y glutelinas presentaron perfiles similares, los resultados sugieren que podría existir una relación con proteínas de almacenamiento 2S, 7S y 11S de origen vegetal. Asimismo, de acuerdo con la FAO/WHO, las fracciones proteínicas tuvieron niveles aceptables para la mayoría de los aminoácidos esenciales, sin embargo, las globulinas y glutelinas fueron deficientes en Lys y Trp. PALABRAS CLAVE: Albúminas -Globulinas -Glutelinas -Jatropha curcas L. -Prolaminas -Proteínas de almacenamiento. SUMMARY Biochemical evaluation of protein fractions from physic nut (Jatropha curcas L.).J. curcas seed proteins were fractioned according to the Osborne method and some biochemical properties were determined for these fractions. Glutelins (378 g kg -1 protein) and globulins (201 g kg -1 protein) were the main components. Albumins and prolamins were the minor components. Protein digestibility was highest in glutelins and globulins with values of 81 and 80% respectively. Electrophoresis analysis showed that globulins and glutelins exhibited similar polypeptide profiles. Electrophoresis patterns suggested that there could be a structural relationship among 2S, 7S and 11S storage proteins from plant sources. According to the FAO⁄WHO reference, the protein fractions had acceptable levels of most of the essential amino acids, but its globulins and glutelins were low in lysine and tryptophan.

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“…Bungkil sisa pengepresan jarak pagar juga diketahui mengandung curcin yang bersifat sangat toksik. Curcin dapat menyebabkan iritasi pada mata, dan keracunan pada hewan peliharaan (Stirpe et al 1976;Devappa et al 2010;Lin et al 2010).…”

Section: Hasil Dan Pembahasanunclassified

Seduhan daun dan buah jarak pagar (Jatropha curcas L.) untuk mengendalikan nematoda perongga akar Radopholus similis secara in vitro

Yousif¹,

Pradana²,

Oktafiyanto³

et al. 2017

Preprint

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Radopholus similis is a nematode that infects many plantation crops. Infected plant roots by nematodes will show necrosis symptoms, then rot due to infection of secondary pathogens. Control of R. similis that is effective, environmentally friendly, and easy to be performed by the farmers is the key to success in reducing losses by this nematode. Extract of physic nut has been recognized to be effective in controlling some important pests, but there is currently no reports of its effectiveness in controlling R. similis. This study aimed to determine the effectiveness of leaves and fruit steeping of physic nut for in vitro control of R. similis. A total of 1:10 (w/v) leaves and fruit of physic nut were steepped, then filtered by a 500 mesh sieve. Steeping was then dripped into a suspension containing 40 R. similis. Twenty-four hours after treatment, the number of dead nematodes was observed. The leaves steeping of physic nut could kill up to 70% of R. similis. Mortality of R. similis treated with steeping of physic nut seeds reached 76.25%. Both steeping applications did not cause damage to the body of nematodes, thus it is presumably that the mortality was due to toxic compounds.

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PURIFICATION AND CHARACTERIZATION OF CURCIN, A TOXIC LECTIN FROM THE SEED OFJATROPHA CURCAS

Cited by 44 publications

References 22 publications

Cytotoxic effect of the immunotoxin constructed of the ribosome-inactivating protein curcin and the monoclonal antibody against Her2 receptor on tumor cells

Cytotoxic effect of the immunotoxin constructed of the ribosome-inactivating protein curcin and the monoclonal antibody against Her2 receptor on tumor cells

Biochemical evaluation of protein fractions from physic nut (<i>Jatropha curcas</i> L.)

Seduhan daun dan buah jarak pagar (Jatropha curcas L.) untuk mengendalikan nematoda perongga akar Radopholus similis secara in vitro

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