Purification and characterization of lipopolysaccharide‐binding protein from hemolymph of American cockroach <i>Periplaneta americana</i>

Jomori, Takahito; Kubo, Takeo; Natori, Shunji

doi:10.1111/j.1432-1033.1990.tb15565.x

Cited by 74 publications

(52 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recognition-specific proteins have been found in some other insects (Söderhall et al, 1988;Ochiai & Ashida, 1988;Richards et al, 1989;Jomori et al, 1990) and one species of crayfish (Duvic & Söderhall, 1990).…”

Section: Discussionmentioning

confidence: 99%

Genetic determinism of the cellular immune reaction in Drosophila melanogaster

Carton¹,

Frey²,

Nappi

1992

Heredity

View full text Add to dashboard Cite

Larvae of Drosophila melanogaster produce a haemocytic reaction against eggs of the parasitoid Leptopilina boulardi, which leads to the formation of a multicellular capsule surrounding the foreign object. Melanization resulting from the conversion of phenol to o-quinones frequently accompanies the cellular reaction. Although various cytological and biochemical aspects of this reaction have been investigated, very little is known about genetic determinism of the insect immune response. The heredity of the capacity to encapsulate was analysed by comparing 16 reciprocal crosses made using inbred resistant and susceptible parental strains. We conclude that differences in the encapsulation capacity of D. melanogaster are inherited autosomally, with the reactive phenotype showing complete dominance over the non-reactive one. There were neither sex-linked nor maternal effects. The results of all crosses suggest a single major segregating locus with two alleles and complete dominance of the resistant allele, with cytoplasmic factors and minor modifying genes acting on the major locus.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic determinism of the cellular immune reaction in Drosophila melanogaster

Carton¹,

Frey²,

Nappi

1992

Heredity

View full text Add to dashboard Cite

show abstract

“…Wilson et al (34) concluded that multiple endogenous serum lectins in the cockroach, Blaberus discoidalis, play important roles in insect innate immunity. Multiple lectins have also been isolated from three other insect species, the American cockroach Periplaneta americana (35)(36)(37)(38), the West Indian leaf cockroach B. discoidalis (34,39,40), and the silkworm B. mori (14,15,(41)(42)(43). In M. sexta, we have identified a family of C-type lectins with different specificities.…”

Section: Binding Of Iml-2 To Lps -A Candidate Ligand For Iml-2 Ismentioning

confidence: 99%

Immulectin-2, a Lipopolysaccharide-specific Lectin from an Insect, Manduca sexta, Is Induced in Response to Gram-negative Bacteria

Kanost

2000

Journal of Biological Chemistry

268

237

View full text Add to dashboard Cite

A lipopolysaccharide-specific lectin, immulectin-2, was isolated from plasma of the tobacco hornworm, Manduca sexta. Immulectin-2 has specificity for xylose, glucose, lipopolysaccharide, and mannan. A cDNA clone encoding immulectin-2 was isolated from an Escherichia coli-induced M. sexta larval fat body cDNA library. The cDNA is 1253 base pairs long, with an open reading frame of 981 base pairs, encoding a 327-residue polypeptide. Immulectin-2 is a member of the C-type lectin superfamily. It consists of two carbohydrate recognition domains, which is similar to the organization of M. sexta immulectin-1. Immulectin-2 was present at a constitutively low level in plasma of control larvae and increased 3-4-fold after injection of Gram-negative bacteria or lipopolysaccharide. Immulectin-2 mRNA was detected in fat body of control larvae, and its level increased dramatically after injection of E. coli. The concentration of immulectin-2 in plasma did not change significantly after injection of Gram-positive bacteria or yeast, even though its mRNA level was increased by these treatments. Compared with immulectin-1, immulectin-2 has a more restricted specificity for binding to Gram-negative bacteria. Immulectin-2 at low physiological concentrations agglutinated E. coli in a calciumdependent manner. It also bound to immobilized lipopolysaccharide from E. coli. Binding of immulectin-2 to lipopolysaccharide stimulated phenol oxidase activation in plasma. The properties of immulectin-2 are consistent with its function as a pattern recognition receptor for detection and defense against Gram-negative bacterial infection in M. sexta.

show abstract

“…LPS is the non-pathogenic, non-living surface molecule derived from E. coli. It is highly immunogenic (Söderhäll 1982;Ratcliffe et al 1985;Jomori et al 1990) and elicits the production of antimicrobial peptides over many hours (Sö derhäll 1982;Ratcliffe et al 1985;Kato et al 1994;Lemaître et al 1996). All injections were made through the pleural membrane between the second and the third abdominal segments, using sterilized glass capillaries that had been pulled out to a fine point.…”

Section: (B) Immune Treatmentsmentioning

confidence: 99%

Adaptive innate immunity? Responsive-mode prophylaxis in the mealworm beetle,Tenebrio molitor

Moret

Siva‐Jothy

2003

Proc. R. Soc. Lond. B

243

210

View full text Add to dashboard Cite

A primary infection by a parasite may indicate a higher risk of being reinfected in the near future (since infection may indicate that enemies are becoming more abundant). Acquired immunity does not exist in invertebrates despite the fact that they also face increased risks of reinfection following primary exposure. However, when subjected to immune insult, insects can produce immune responses that persist for long enough to provide prophylaxis. Because these immune responses are costly, persistence must be maintained through a selective advantage. We tested for the possibility that these long-lasting immune responses provided increased resistance to later infections by experimentally mimicking a primary immune insult (pre-challenge) in larvae of the mealworm beetle, Tenebrio molitor, with lipopolysaccharides (LPS) prior to early or late exposure to spores of the entomopathogenic fungus Metarhizium anisopliae. We found that pre-challenged larvae produced a long-lasting antimicrobial response, which provided a survival benefit when the larvae were exposed to fungal infection. These results suggest that the observed response is functionally 'adaptive'.

show abstract

Purification and characterization of lipopolysaccharide‐binding protein from hemolymph of American cockroach Periplaneta americana

Cited by 74 publications

References 39 publications

Genetic determinism of the cellular immune reaction in Drosophila melanogaster

Genetic determinism of the cellular immune reaction in Drosophila melanogaster

Immulectin-2, a Lipopolysaccharide-specific Lectin from an Insect, Manduca sexta, Is Induced in Response to Gram-negative Bacteria

Adaptive innate immunity? Responsive-mode prophylaxis in the mealworm beetle,Tenebrio molitor

Contact Info

Product

Resources

About