Purification and Characterization of Peach Mosaic Virus

Gispert, Carmen; Perring, Thomas M.; Creamer, Rebecca

doi:10.1094/pdis.1998.82.8.905

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…Procedures reported for the purification of Maize stripe virus (MSpV) (40), the nucleoprotein of Tomato spotted wilt virus (9), Peach mosaic virus (16), and BRV (33), and a minipurification protocol of Lane (32) were evaluated for purification from SMD-affected pigeonpea. At various steps in the purification procedures, preparations were monitored by electron microscopy, polyacrylamide gel electrophoresis (PAGE), and ELISA for PPSMV.…”

Section: Methodsmentioning

confidence: 99%

“…Several other eriophyid mite-transmitted disease agents infecting dicotyledonous plants also have remained intractable to study for many decades (38) but, in the last few years, progress has been made in identifying the causal agents of three such diseases (16,18,19,33). With the exception of Blackcurrant reversion virus (BRV), all known mite-transmitted viruses have flexuous rodshaped or filamentous particles (16,18,19,33).…”

mentioning

confidence: 99%

“…With the exception of Blackcurrant reversion virus (BRV), all known mite-transmitted viruses have flexuous rodshaped or filamentous particles (16,18,19,33). Eriophyid mites, particularly A. cajani, possesses a short stylet of 2.03 µm that permits penetration into only epidermal and, at most, underlying mesophyll cells (44).…”

mentioning

confidence: 99%

“…Usually, viruses occurring in such tissues are transmitted mechanically in sap extracts. Indeed, all the characterized mite-transmitted viruses (BRV, High plains virus [HPV], Peach mosaic virus, and PPSMV) are transmissible in this way although, for some, only with great difficulty (16,18,21,33,38).…”

mentioning

confidence: 99%

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A Novel Mite-Transmitted Virus with a Divided RNA Genome Closely Associated with Pigeonpea Sterility Mosaic Disease

Kumar¹,

Jones²,

Reddy³

2003

Phytopathology®

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Novel Mite-Transmitted Virus with a Divided RNA Genome Closely Associated with Pigeonpea Sterility Mosaic Disease

Kumar¹,

Jones²,

Reddy³

2003

Phytopathology®

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“…For example, the characterization of the mitetransmitted Blackcurrant reversion associated virus (BRAV) was achieved after transmitting it to herbaceous hosts (13), whereas Peach mosaic virus (PMV) was characterized after purification directly from the natural woody host (4). We used a protocol to transfer the potential viral agent of PSMD to herbaceous hosts on the assumption that it exists in low titer and is distributed erratically in pigeonpea and that host polyphenolic compounds interfere with virus extraction and/or infectivity.…”

Section: Characterization Of a Virus From Pigeonpea With Affinities Tmentioning

confidence: 99%

Characterization of a Virus from Pigeonpea with Affinities to Species in the Genus Aureusvirus, Family Tombusviridae

Kumar

Jones²,

Sreenivasulu

et al. 2001

Plant Disease

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In attempts to identify the causal agent of pigeonpea sterility mosaic disease (PSMD), which is transmitted by eriophyid mites, a virus was isolated with great difficulty from some PSMD-affected pigeonpea (Cajanus cajan) plants from different locations in India. Once isolated from pigeonpea, the virus was transmitted readily by mechanical inoculation to several herbaceous species, reaching very high concentrations in some species. The virus was transmitted experimentally through soil to herbaceous test plants but not to pigeonpea. When virus particles were purified and inoculated mechanically to healthy pigeonpea, the virus induced necrosis in inoculated leaves only and did not spread systemically. Therefore, the virus is not the causal agent of PSMD. The virus has isometric particles approximately 30 nm in diameter that sediment as a single component and had a buoyant density in CsCl and Cs2SO4 of 1.34 and 1.27 g·cc-1, respectively. Purified virus particle preparations contained a single major protein of approximately 44 kDa and three RNA species of approximately 4,300, 2,700, and 1,500 nucleotides. Only the largest RNA species was infective to plants; the two smaller species were encapsidated subgenomic species of the 3′ end of the larger genomic RNA. The viral genome was sequenced and showed 93% homology to that of Pothos latent virus (PoLV), a recently described virus in the genus Aureusvirus, family Tombusviridae, and was indistinguishable from PoLV in gel double-diffusion serological tests. This virus, therefore, is regarded as a pigeonpea isolate of PoLV (PoLV-PP). In field studies in different locations in India, enzyme-linked immunosorbent assay and reverse-transcriptase polymerase chain reaction detected PoLV-PP in 10.7% of PSMD-affected and 8.1% of asymptomatic pigeonpea plants. The significance of these findings is discussed.

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Detection and Identification of Viruses and Viroids

Narayanasamy

2017

Microbial Plant Pathogens

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Purification and Characterization of Peach Mosaic Virus

Cited by 13 publications

References 16 publications

A Novel Mite-Transmitted Virus with a Divided RNA Genome Closely Associated with Pigeonpea Sterility Mosaic Disease

A Novel Mite-Transmitted Virus with a Divided RNA Genome Closely Associated with Pigeonpea Sterility Mosaic Disease

Characterization of a Virus from Pigeonpea with Affinities to Species in the Genus Aureusvirus, Family Tombusviridae

Detection and Identification of Viruses and Viroids

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