Purification and Characterization of the Bacteriocin Thuricin Bn1 Produced by Bacillus thuringiensis subsp. kurstaki Bn1 Isolated from a Hazelnut Pest

Uğraş, Serpil; Sezen, Kazım; Katı, Hatice; Demirbağ, Zihni

doi:10.4014/jmb.1209.09056

Cited by 37 publications

(25 citation statements)

References 35 publications

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“…The six seaweed-derived Bacillus investigated in the present study were selected based on antimicrobial activity against Gram-negative pathogens [21]. This may be due to the production of bacteriocins [20], as although Bacillus bacteriocins are reportedly more active against Gram-positive bacteria, some have a broader spectrum that also includes Gram-negatives [30,31]. However, additional probiotic properties and safety must be evaluated prior to in vivo use.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics

et al. 2014

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Six antimicrobial-producing seaweed-derived Bacillus strains were evaluated in vitro as animal probiotics, in comparison to two Bacillus from an EU-authorized animal probiotic product. Antimicrobial activity was demonstrated on solid media against porcine Salmonella and E. coli. The marine isolates were most active against the latter, had better activity than the commercial probiotics and Bacillus pumilus WIT 588 also reduced E. coli counts in broth. All of the marine Bacillus tolerated physiological concentrations of bile, with some as tolerant as one of the probiotics. Spore counts for all isolates remained almost constant during incubation in simulated gastric and ileum juices. All of the marine Bacillus grew anaerobically and the spores of all except one isolate germinated under anaerobic conditions. All were sensitive to a panel of antibiotics and none harbored Bacillus enterotoxin genes but all, except B. pumilus WIT 588, showed some degree of β-hemolysis. However, trypan blue dye exclusion and xCELLigence assays demonstrated a lack of toxicity in comparison to two pathogens; in fact, the commercial probiotics appeared more cytotoxic than the majority of the marine Bacillus. Overall, some of the marine-derived Bacillus, in particular B. pumilus WIT 588, demonstrate potential for use as livestock probiotics.

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Section: Discussionmentioning

confidence: 99%

In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics

et al. 2014

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“…• C (6400 AU/mL) ( Table 6) (Ugras et al 2013). With regard to Bt-Bn1, the best medium and incubation time for the antimicrobial substance production was determined to be MHB and 24 h (antibacterial activity is the same as for 48 h, however, shorter time was preferred) with antimicrobial substance activity being 120 AU/mL (Table 4).…”

Section: Resultsmentioning

confidence: 99%

“…The production kinetics of these antibacterial substances produced by the Sm-Mm3, Bt-Bn1 (Ugras et al 2013) and Bp-Ar2 were similarly determined as primary metabolite. The production kinetics of these substances is similar to bacteriocins.…”

Section: Discussionmentioning

confidence: 99%

Screening antibacterial activity of entomopathogenic bacteria isolated from pests of hazelnut

Uğraş

Demirbağ

2013

Biologia

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Thirty-seven entomopathogenic bacteria isolated from six common pests of hazelnut in the Black Sea Region of Turkey have been screened for their potential of antibacterial substance production against indicator bacteria by the agar spot assay and well diffusion assay. Results indicated that 13.5% of entomopathogenic bacteria, Pseudomonas fluorescens (Pf-Xd1), Bacillus polymyxa (Bp-Ar2), Bacillus thuringiensis (Bt-Bn1), Serratia marcescens (Sm-Mm3) and Pseudomonas flourescens (Pf-Aa4) isolated from pests of Xyleborus dispar, Anoplus roboris, Balaninus nucum, Melolontha melolontha and Agelastica alni, respectively, showed significant levels of inhibitor activities against indicator bacteria. Well diffusion assay showed that supernatants of Bp-Ar2, Bt-Bn1 and Sm-Mm3 have antibacterial activity, whereas Pf-Xd1 and Pf-Aa4 did not show any activity. Furthermore, the antibacterial activity of the substance produced by the Bp-Ar2 has a narrow spectrum, whereas those of Bt-Bn1 and Sm-Mm3 exhibit broad spectrum. The production of these antibacterial substances were similarly determined at early logarithmic phase in the growth cycle of three bacteria and continued until the beginning of the stationary phase as primer metabolite. In addition, optimal pH (at 7-9 forBt-Bn1 and 5-9 forSm-Mm3), medium (Muller Hinton broth forBt-Bn1 and Luria Bertani broth forSm-Mm3), temperature (25 • C forBt-Bn1 and Sm-Mm3) and production time (24h forBt-Bn1 and 72h forSm-Mm3) of these substances were determined. Our results demonstrate that entomopathogenic bacteria are a potential source of antibacterial substances.

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“…kurstaki Bn1 isolated from hazel nut pest Balaninus nucum L. produces a bacteriocin Bt-Bn1, the first of its kind of insect origin. Like its potential commercial counterparts, this bacteriocin has antibacterial activity against B. cereus , P. syringae , and P. lemoignei , and other B. thuringiensis strains ( Ugras et al, 2013 ).…”

Section: Bacteriocins From Other Interesting Bacterial Sourcesmentioning

confidence: 99%

Bacteriocins from the rhizosphere microbiome – from an agriculture perspective

Subramanian

Smith

2015

Front. Plant Sci.

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Bacteria produce and excrete a versatile and dynamic suit of compounds to defend against microbial competitors and mediate local population dynamics. These include a wide range of broad-spectrum non-ribosomally synthesized antibiotics, lytic enzymes, metabolic by-products, proteinaceous exotoxins, and ribosomally produced antimicrobial peptides (bacteriocins). Most bacteria produce at least one bacteriocin. Bacteriocins are of interest in the food industry as natural preservatives and in the probiotics industry, leading to extensive studies on lactic acid bacteria (colicin produced by Escherichia coli is a model bacteriocin). Recent studies have projected use of bacteriocins in veterinary medicine and in agriculture, as biostimulants of plant growth and development and as biocontrol agents. For example, bacteriocins such as Cerein 8A, Bac-GM17, putidacin, Bac 14B, amylocyclicin have been studied for their mechanisms of anti-microbial activity. Bac IH7 promotes tomato and musk melon plant growth. Thuricin 17 (Th17) is the only bacteriocin studied extensively for plant growth promotion, including at the molecular level. Th17 functions as a bacterial signal compound, promoting plant growth in legumes and non-legumes. In Arabidopsis thaliana and Glycine max Th17 increased phytohormones IAA and SA at 24 h post treatment. At the proteome level Th17 treatment of 3-week-old A. thaliana rosettes led to >2-fold changes in activation of the carbon and energy metabolism pathway proteins, 24 h post treatment. At 250 mM NaCl stress, the control plants under osmotic-shock shut down most of carbon-metabolism and activated energy-metabolism and antioxidant pathways. Th17 treated plants, at 250 mM NaCl, retained meaningful levels of the light harvesting complex, photosystems I and II proteins and energy and antioxidant pathways were activated, so that rosettes could better withstand the salt stress. In Glycine max, Th17 helped seeds germinate in the presence of NaCl stress, and was most effective at 100 mM NaCl. The 48 h post germination proteome suggested efficient and speedier partitioning of storage proteins, activation of carbon, nitrogen and energy metabolisms in Th17 treated seeds both under optimal and 100 mM NaCl. This review focuses on the bacteriocins produced by plant-rhizosphere colonizers and plant-pathogenic bacteria, that might have uses in agriculture, veterinary, and human medicine.

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Purification and Characterization of the Bacteriocin Thuricin Bn1 Produced by Bacillus thuringiensis subsp. kurstaki Bn1 Isolated from a Hazelnut Pest

Cited by 37 publications

References 35 publications

In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics

In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics

Screening antibacterial activity of entomopathogenic bacteria isolated from pests of hazelnut

Bacteriocins from the rhizosphere microbiome – from an agriculture perspective

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