Purification and Characterization of the Major Envelope Glycoprotein of Simian Foamy Virus Type 1

Sensitive Assays for Isolation and Detection of Simian Foamy Retroviruses

“…5A, lane 1). Some of the proteins were comparable in size to those previously reported for the SFV-1 and SFV-3 Gag, Pol, and Env proteins, e.g., 134 kDa for the Pol precursor (35), 77 kDa for the RT (80 kDa) (8), 117 kDa for the Env precursor (27), 71 kDa for the Env glycoprotein (70 kDa) (7,9) and 70 kDa for the Gag precursor (69 kDa) (27). Small amounts of the gag-encoded major core protein (30 kDa) (8,9) were detected, indicating inefficient processing of the precursor proteins.…”

Identification of the Major Immunogenic Structural Proteins of Human Foamy Virus

“…In [35S]methionine-labelled lysates of pelleted virus, only gpl30 and not gp 170 seemed to be present, and gp47 constituted one of the major antigens, thereby adding support to this view. However, findings of Cavalieri et aL (1981) and Benzair et al (1985) for the closely related simian foamy virus type 1 (SFV-1 ; Mergia et al, 1990), are in contrast to our results; they described two presumed SFV-1 env proteins, gp70 (surface protein) and p30 (transmembrane protein), respectively. We failed to identify a glycosylated HFV-specific protein in the range of 70K.…”

Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes

“…wts of foamy viral glycoprotein precursors are unknown. The single report by Benzair et al (1985) of a major env glycoprotein of simian foamy virus type 1 of 70 kd cannot directly be compared to the OMP of the HSRV, since the un-2082 modified protein sequence predicts a value of 53.7 kd. However, the value of 53.7 kd is consistent with the reported one, since it is a generat characteristic for most retrovirus that the mol.…”

“…This holds also true for the matlire OMP of HSRV that contains 12 potential glycosylation signals which would account for the difference in mol. wts neglecting that a spumavirus from a different species was used in that report (Benzair et al , 1985).…”