Purification and Characterization of Thermostable Chitinase from Bacillus SW41 for Chitin Oligomer Production

Wahyuni, S.; Suhartono, Maggy Thenawidjaja; Khaeruni, Andi; Purnomo, Adi Setyo; Asranudin,; Holilah, Holilah; Riupassa, Pieter Agusthinus

doi:10.14233/ajchem.2016.20099

Cited by 20 publications

(10 citation statements)

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“…The R. pickettii culture was maintained on a nutrient agar (NA, Difco) incubated at 37 o C and transferred into 100-ml Erlenmeyer flasks containing 50 ml nutrient broth (NB, Difco, UK). The cultures were also pre-incubated for 30 h at 37 o C with shaker (11 ×g) [41,42].…”

Section: Bacteria Culture Conditionsmentioning

confidence: 99%

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Purnomo¹,

Maulianawati²,

Kamei³

2019

Journal of Microbiology and Biotechnology

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DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactantproducing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml (1 ml ≈ 1.44 × 10 1 3 CFU). After 7 d incubation, about 43% of the initial DDT (12.5 µM) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.

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Section: Bacteria Culture Conditionsmentioning

confidence: 99%

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Purnomo¹,

Maulianawati²,

Kamei³

2019

Journal of Microbiology and Biotechnology

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“…The colony was inoculated into 40 ml of nutrient broth (NB; Difco) medium in 50-ml Falcon flasks. The cultures were pre-incubated at 37°C for 12 h with a shaker (180 rpm) [30].…”

Section: Bacteria Culture Conditionsmentioning

confidence: 99%

Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation

Purnomo¹,

Ashari²,

Hermansyah³

2017

Journal of Microbiology and Biotechnology

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DDT (1,1,1-trichloro-2,2-(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, , and biosurfactant-producing bacteria, and , on DDT biodegradation. Bacteria were added into the culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 10 bacteria cells/ml culture). DDT was degraded to approximately 19% by during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of into culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2-(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by and. The results of this study indicate that has a synergistic relationship with and can be used to optimize the degradation of DDT by .

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“…Cocultures at 1 and 3 ml, showed that the concentration of P. aeruginosa was limited to be able to enhance the DDT removal processed by P. eryngii. Another possibility was that P. aeruginosa and P. eryngii produce some metabolite that inhibits each other to degrade DDT (Wahyuni et al 2016;Ramadhania et al 2018;Sariwati et al 2019). Generally, the addition of P. aeruginosa enhanced the degradation rate of DDT by P. eryngii, but there is no synergistic relationship between P. aeruginosa and P. eryngii.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa

2021

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Dichloro-diphenyl-trichloroethane (DDT) is a synthetic insecticide that widely used around the world, which has a negative effect on human health and the environment. The objective of this research was to investigate the ability of bacterium Pseudomonas aeruginosa in co-culturing with white-rot fungus Pleurotus eryngii to degrade DDT. The various volume of P. aeruginosa (1 ml ≈ 1.5 x 109 CFU) were added into 10 ml of P. eryngii culture for a 7-days of incubation. Approximately 82% of degradation of DDT were obtained from co-cultures with the adjunct of 10 ml of P. aeruginosa during the 7-day incubation period, which had the best ratio of optimization of 0.57. The confrontational assay showed that P. aeruginosa gave no effect on the growth of P. eryngii (0.39 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by co-cultures bacterium P. aeruginosa and fungus P. eryngii. This study indicated that bacterium P. aeruginosa can be used to enhance DDT degradation by whire-rot fungus P. eryngii.

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Purification and Characterization of Thermostable Chitinase from Bacillus SW41 for Chitin Oligomer Production

Cited by 20 publications

References 0 publications

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation

Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa

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