Purification and identification of three novel antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) skin

Chi, Chang-Feng; Wang, Bin; Hu, Fa-Yuan; Wang, Yumei; Zhang, Bin; Deng, Shanggui; Wu, Changwen

doi:10.1016/j.foodres.2014.08.038

Cited by 138 publications

(96 citation statements)

References 27 publications

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“…However, this result was in accordance with the result of Pan et al () that peptide fraction derived from the elution with 1.0 M NaCl had lower radical scavenging than that one eluted with 0.5 M NaCl. In addition, Chi et al () found that the peptide fraction that was eluted with 0.5 M NaCl had the highest radical scavenging activity with EC 50 values between 0.368 and 1.716 mg/ml. Therefore, F2‐A was pooled and collected for the next fractionation with size‐exclusion chromatography.…”

Section: Resultsmentioning

confidence: 99%

Fractionation and characterization of antioxidant peptides from rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion

Phongthai

Rawdkuen

2020

Cereal Chem

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Background and objective Rice bran protein concentrate was prepared and hydrolyzed by using pepsin–pancreatin. The obtained result was further filtered through 5‐kDa membranes. A permeate was collected and sequentially fractionated by different chromatographic techniques. The peptide fractions were determined for the antioxidant activities. Findings Three peptide fractions were derived after separating the crude protein hydrolysates by anion‐exchange chromatography. Due to its highest ABTS radical scavenging and metal chelating activities (p < .05), fraction F2‐A was pooled and separated further by size‐exclusion chromatography (SEC). Among the SEC fractions, F1‐S showed strongest antioxidant activities; thus, it was collected for reverse‐phase HPLC purification. Subsequently, the peptide fractions were successfully separated and F4‐H had the greatest ABTS‐scavenging activities (214.61 µmol Trolox/g sample, respectively). In addition, it displayed the strongest ability to reduce ferric to ferrous ions (104.80 µmol FeSO4/g sample) (p < .05), while F1‐H showed the highest ability to chelate metal ions (266.40 µmol EDTA/g sample) (p < .05). Conclusion These results demonstrate that hydrophobic amino acids play a major role in scavenging free radicals, whereas polar amino acids are responsible for reducing and chelating metal ions. Significance and novelty Rice bran protein composes of high ratio of hydrophobic amino acid. It is a suitable source of antioxidant peptides that might be produced during gastrointestinal digestion. Separation and purification using chromatographic techniques showed promising results for selectively fractionate antioxidant peptides with specific activity.

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Section: Resultsmentioning

confidence: 99%

Fractionation and characterization of antioxidant peptides from rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion

Phongthai

Rawdkuen

2020

Cereal Chem

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“…On the one hand, the hydrophobic and aromatic amino acids play an important role in antioxidant activity of peptide. The peptides containing hydrophobic amino acids, such as Val, Trp, Tyr, Leu, Ile, and Pro, have been reported to exert significant radical scavenging activity (Chi et al, ). The amino acids with aromatic residues (Phe, Trp, and Tyr) could convert radical to stable molecule by donating electron (Sarmadi & Ismail, ).…”

Section: Resultsmentioning

confidence: 99%

“…The DPPH radical scavenging rate of these five fractions is showed in Figure 6b. (Chi et al, 2015). The amino acids with aromatic residues (Phe, Trp, and Tyr) could convert radical to stable molecule by donating electron (Sarmadi & Ismail, 2010).…”

Section: Purification and Characterization Of Peptidementioning

confidence: 99%

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Hao

Jiang

et al. 2020

J Food Biochem

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The soft part of Asian clam (Corbicula fluminea) was hydrolyzed using trypsin and the hydrolysates were separated by ultrafiltration using the membrane with molecular weight cutoff of 10 and 5 kDa. Three fractions (F10000, F10000–5000, and F5000) were obtained. The F5000 showed the strongest scavenging abilities to DPPH (2,2‐diphenyl‐1‐picrylhydrazyl), superoxide anion, and hydroxyl radicals, and was further fractionated into four fractions (component I, II, III, and IV) by gel chromatography. The protective effects of these components on oxidative damage induced by hydrogen peroxide (H2O2) in HepG2 cells were evaluated. Meanwhile, component I separated from F5000 had more positive effects on increasing the activity of catalase, decreasing lipid peroxidation, inhibiting H2O2‐induced apoptosis, and higher yield. The component I was further separated using anion exchange chromatography and reversed phase high‐performance liquid chromatography, and the peptide sequence of Lys‐Gly‐Pro‐Ala‐ Pro‐Phe‐Tyr‐Pro‐Leu was identified by mass with molecular weight of 988.3 Da. Practical applications Asian clam (C. fluminea) is used for liver protection in traditional Chinese medicine. The present study investigated the radical scavenging activity of the hydrolysates from C. fluminea and the protective effects of the hydrolysate fractions on oxidative damage induced by H2O2 in HepG2 cells. In addition, a peptide was purified from the hydrolysate and the amino acid sequence of it was identified. Although chemical antioxidant has some side effects on health, the peptide with antioxidant activity obtained from C. fluminea would have more extensive application in food and nutraceutical.

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“…Therefore, the purified S. platensis protein in this study was directly digested by several proteolytic enzymes and filtrated to molecular weight cut off < 3 kDa. Several studies have previously reported that lower molecular weight shows higher free-radical scavenging activity due to their rich amino acid groups for donating electrons to DPPH radicals (Chi et al, 2015;Zhang et al, 2011). Moreover, Ngoh and Gan (2016) suggested that the peptide < 3 kDa from pinto bean protein hydrolysates exhibited the highest antioxidant activities than 100, 50, 30, and 10 kDa.…”

Section: Antioxidant Activities Of S Platensis Protein Hydrolysatesmentioning

confidence: 98%

Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates

Safitri

Herawati

Hsu

2017

RJLS

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The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS-MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide PheSer-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.

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Purification and identification of three novel antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) skin

Cited by 138 publications

References 27 publications

Fractionation and characterization of antioxidant peptides from rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion

Fractionation and characterization of antioxidant peptides from rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates

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