Purification and physico-chemical properties of Penicillium tardum α-L-rhamnosidase

The influence of coordinative tartrate and malatogermanate compounds on the activity of ?-L-rhamnosidase preparations from Penicillium tardum, Eupenicillium erubescens and Cryptococcus albidus

“…Whereas, in the experiment with e. erubescens, the picture changed: the same compound was shown to act as activator and its effect did not depend on the concentration and increased with time. Something similar was observed for compounds (1) and (4). They inhibited the enzyme activity from P. tardum, did not 4is an inhibitor.…”

“…In the experiments with the enzyme from e. erubescens, along with compound (3), significant activity was also observed for compounds (6) and (8), which do not contain d-metal cations, but are able to bind to the active site of a metal-containing α-L-rhamnosidase. Differences in the influence of (1)-(11) on the activity of the enzymes from P. tardum, e. erubescens and C. albidus indicate that activation and inhibition occur with the involvement of different functional groups of these enzymes.…”

“…The preparations of α-L-rhamnosidases were obtained from the culture supernatant of P. tardum [4], e. erubescens [5] and C. albidus [6], by filtration through 4 layers of gauze (for e. erubescens and P. tardum) or by centrifugation (for C. albidus), after separation of biomass and precipitation with ammonium sulfate to 90% saturation. The mixture was kept for 12-16 h at 4 °C and then centrifuged at 5000 g for 30 min.…”

“…With this in mind, we searched for effective producers of highly specific α-L-rhamnosidases among the strains of doi: https://doi.org/10.15407/ubj92.04.085 micro organisms of various taxonomic groups -bacteria, micromycetes, and yeast from the depositary of IMV, NAS of Ukraine. We selected the most active strains -Penicillium tardum [4], eupenicillium erubescens [5] and Cryptococcus albidus [6] and obtained α-L-rhamnosidase preparations from their culture fluids. Then we studied their physiochemical properties, substrate specificity, and functional groups involved in the catalytic process [7][8][9].…”

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“…Раніше [1] в результаті скринінгу із колекції живих культур відділу фізіології та систематики мікроміцетів ІМВ НАН України нами був відібраний активний штам Penicillium tardum, вивчені його трофічні особливості та підібрані оптимальні умови культивування [2]. З супернатанту культуральної рідини P. tardum виділено і очищено препарат α-L-рамнозидази, вивчено його фізико-хімічні властивості [3]. Разом з тим для створення високоефективної технології отримання ензимних препаратів необхідне дослідження оптимальних параметрів α-L-рамнозидазної реакції, а також механізму дії ензиму.…”