Purification and Utilization of Gum from Terminalia Catappa L. for Synthesis of Curcumin Loaded Nanoparticle and Its In Vitro Bioactivity Studies

Purification, characterization and exploitation of Azadirachta indica gum for the production of drug loaded nanocarrier

“…Antioxidant assays such as DPPH assay (Radical Scavenging assay) (Maizura et al 2011), FRAP (Ferric Reducing Antioxidant Power) assay (Benzie and Strain 1996) and Phosphomolybdenum assay (Saha et al 2008) were also performed to identify the antioxidant properties of the extracted polysaccharide. Anticancer activity of polysaccharide against MCF7 (Michigan Cancer Foundation-7) human breast cancer cell line was evaluated by MTT assay (Mosmann (1983)) and apoptosis for IC 50 (Inhibition Concentration 50) value obtained by MTT assay was detected by Acridine Orange -Ethidium Bromide staining (AO/EB) (Samrot et al 2018a). Pathological changes in cancer cells were observed and recorded using fluorescence microscope (Thermofisher).…”

“…The pellets were washed with ethanol and were subjected to dialysis against deionized water for 3 days. It was then subjected to lyophilization (Dodi et al 2011, Samrot et al 2018a.…”

“…The nanocarriers were collected through centrifugation. The supernatant was discarded and the pellets were lyophilized (Samrot et al 2018a). This results in four different nanocarriers -CMAI 1.1 (0.05 g of CMAI-1 and 0.02 g of STMP), CMAI 1.2 (0.02 g of CMAI-1 and 0.01 g of STMP), CMAI 2.1 (0.05 g of CMAI-2 and 0.02 g of STMP), CMAI 2.2 (0.02 g of CMAI-2 and 0.01 g of STMP).…”

“…The drug (curcumin) solution was prepared by dissolving 15 mg of the curcumin in 10 ml of ethanol. 25 mg nanocarriers dispersed in 10 ml deionized water was added to the drug solution with continuous stirring at room temperature for 15 min, following that it was subjected to high frequency water bath sonicator for about 30 min (Samrot et al 2018a). The obtained solution was subjected to centrifugation and washed thrice with Millipore water and lyophilized.…”

“…Polysaccharides can be used as nanocarrier to carry drugs, which is possible when the polysaccharides are carboxymethylated and chelated with an appropriate chelator like STMP (sodium tri metaphosphate), STPP (sodium tripolyphosphate) etc , Samrot et al 2018a, Shobana et al 2019. These polysaccharide nanocarriers have attracted attention of various researchers in the field of medical field for drug delivery and drug encapsulation (Justin et al 2017, Justin et al 2018, Sruthi et al 2019.…”