Purification, characterization, and antitumor activity of nonrecombinant mouse tumor necrosis factor.

Haranaka, Katsuyuki; Carswell, Elizabeth A.; Williamson, Barbara; Prendergast, Jay S.; Satomi, Nobuko; Old, Lloyd J.

doi:10.1073/pnas.83.11.3949

Cited by 71 publications

(39 citation statements)

References 28 publications

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“…The mol. wt of murine TNF has been estimated by gel filtration to be -40kD (Haranaka et al, 1986) and -70-80kD (Beutler et al, 1985;Kull et al, 1984); our results are concordant with the latter. The different estimates of the molecular size of murine TNF may be attributable to differences in the conditions of gel filtration.…”

Section: Characterization Of Cf Induced In Tumour Tissuessupporting

confidence: 82%

Local induction of a cytotoxic factor in a murine tumour by systemic administration of an antitumour polysaccharide, MGA

Takahashi

Watanuki²,

Yamazaki³

et al. 1988

Br J Cancer

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When an antitumour mannoglucan prepared from Microellobosporia grisea, MGA was administered i.v. to C3H/He mice bearing the solid MH134 hepatoma, a cytotoxic factor was induced that was detectable in the tumour homogenate by an 8 h cytolysis assay against L-929 fibroblasts. Without MGA treatment, the cytotoxic factor was not detectable in the tumour homogenate. MGA induced the cytotoxic factor in tumour tissue specifically, its level reaching a maximum (24 U ml-1) 3 h after administration of MGA: little if any cytotoxic factor was detectable in homogenates of normal tissues or sera after MGA-treatment. The molecular size of the cytotoxic factor was estimated to be 70-80 kD by gel filtration. It seemed to be a type of tumour necrosis factor because its activity was inhibited by antiserum against murine tumour necrosis factor. From these results, the selective induction of the cytotoxic factor was concluded to be important in the mechanism of the antitumour activity of MGA.

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Section: Characterization Of Cf Induced In Tumour Tissuessupporting

confidence: 82%

Local induction of a cytotoxic factor in a murine tumour by systemic administration of an antitumour polysaccharide, MGA

Takahashi

Watanuki²,

Yamazaki³

et al. 1988

Br J Cancer

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“…Our experiments focus on what is required for eradication of large, established, solid cancers; i.e., the prevention of outgrowth of ALVs. TNF is one of very few cytokines that cause dramatic destruction of large, established tumors (58). For induction of necrosis in large, established tumors by paratumoral injection of recombinant TNF, TNFRs needed to be expressed only on non-BM stromal cells (59).…”

Section: Discussionmentioning

confidence: 99%

IFN-γ– and TNF-dependent bystander eradication of antigen-loss variants in established mouse cancers

Zhang¹,

Karrison²,

Rowley³

et al. 2008

J. Clin. Invest.

145

146

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Tumors elicit antitumor immune responses, but over time they evolve and can escape immune control through various mechanisms, including the loss of the antigen to which the response is directed. The escape of antigenloss variants (ALVs) is a major obstacle to T cell-based immunotherapy for cancer. However, cancers can be cured if both the number of CTLs and the expression of antigen are high enough to allow targeting of not only tumor cells, but also the tumor stroma. Here, we showed that IFN-γ and TNF produced by CTLs were crucial for the elimination of established mouse tumors, including ALVs. In addition, both BM-and non-BM-derived stromal cells were required to express TNF receptors and IFN-γ receptors for the elimination of ALVs. Although IFN-γ and TNF were not required by CTLs for perforin-mediated killing of antigen-expressing tumor cells, the strong inference is that tumor antigen-specific CTLs must secrete IFN-γ and TNF for destruction of tumor stroma. Therefore, bystander killing of ALVs may result from IFN-γ and TNF acting on tumor stroma.

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“…Neutralizing anti-rabbit TNF-␣ monoclonal antibody (mAb; a generous gift from Dr. H. Nariuchi, The University of Tokyo, Japan) and anti-rabbit MCP-1 mAb were purified as described [21,22]. The antibodies used in this study did not cross-react with other cytokines.…”

Section: Methodsmentioning

confidence: 99%

Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell-mediated pulmonary granulomatosis in rabbits

Ichiyasu

Suga

Matsukawa

et al. 1999

Journal of Leukocyte Biology

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Purification, characterization, and antitumor activity of nonrecombinant mouse tumor necrosis factor.

Cited by 71 publications

References 28 publications

Local induction of a cytotoxic factor in a murine tumour by systemic administration of an antitumour polysaccharide, MGA

Local induction of a cytotoxic factor in a murine tumour by systemic administration of an antitumour polysaccharide, MGA

IFN-γ– and TNF-dependent bystander eradication of antigen-loss variants in established mouse cancers

Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell-mediated pulmonary granulomatosis in rabbits

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