Purification, Characterization, and Molecular Cloning of a Novel Antifungal Lectin From the Roots of Ophioglossum pedunculosum

He, Xiaoying; Ji, Na; Xiang, Xiaocong; Luo, Peng; Bao, Ji

doi:10.1007/s12010-011-9367-z

Cited by 18 publications

(6 citation statements)

References 43 publications

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“…Ganapati et al [23] studied the lectin of R. vivipara and demonstrated that it is stable in a pH range of 2 to 9. Lectin isolated from Ophioglossum pedunculosum maintained its HA at pH from 4-8 [34].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Braga

Lacerda

Medeiros

et al. 2014

Appl Biochem Biotechnol

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The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its glycoprotein nature, and sugar specificity, as well as resistance to pH, temperature, denaturing agents, reduction, oxidation, and chelation. A microdilution method was used to determine antibacterial activity, and hemolytic activity was observed in human erythrocytes. The SFL has a molecular weight of 17 kDa, and a carbohydrate content of 53 μg/mL, specific for arabinose and xylose, and is resistant to treatment with urea, sensitive to treatment with sodium metaperiodate and β-mercaptoethanol, and in the presence of EDTA lost its hemagglutinating activity (HA). However, in the presence of divalent cations (Ca(2 +) and Mn(2 +)) the HA was increased. The SFL remained active even after incubation at 80 °C, and, within pH values of between 5 and 11. The SFL inhibited the bacterial growth of all the tested strains and caused little hemolysis in human erythrocytes when compared to the positive control Triton X-100.

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Section: Resultsmentioning

confidence: 99%

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Braga

Lacerda

Medeiros

et al. 2014

Appl Biochem Biotechnol

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“…Homoflavonoids from O. petiolatum have antihepatitis B virus activity (Lin et al 2005b;Wan et al 2013). O. pedunculosum contains 3-O-methylquercetin glucosides that inhibit lipopolysaccharideinduced NO production in macrophages (Wan et al 2012), and a mannan-specific lectin with antifungal activity (He et al 2011). An alcohol-ethyl acetate fraction from O. thermale has shown anti-inflammatory effects on carrageenan-induced inflammation, which may be related to the extract's antioxidant properties (Zhang et al 2012d).…”

Section: Ophioglossaceaementioning

confidence: 99%

Phytochemicals from fern species: potential for medicine applications

et al. 2017

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Ferns are an important phytogenetic bridge between lower and higher plants. Historically they have been used in many ways by humans, including as ornamental plants, domestic utensils, foods, and in handicrafts. In addition, they have found uses as medicinal herbs. Ferns produce a wide array of secondary metabolites endowed with different bioactivities that could potentially be useful in the treatment of many diseases. However, there is currently relatively little information in the literature on the phytochemicals present in ferns and their pharmacological applications, and the most recent review of the literature on the occurrence, chemotaxonomy and physiological activity of fern secondary metabolites was published over 20 years ago, by Soeder (Bot Rev 51:442–536, 1985). Here, we provide an updated review of this field, covering recent findings concerning the bioactive phytochemicals and pharmacology of fern species

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“…Lectins from Phaseolus vulgaris seeds inhibited the growth of Coprinus comatus and Rhizoctonia solani [88] as well as Valsa mali [89]. A mannan-specific lectin from Ophioglossum pedunculosum roots strongly damaged the growth of Sclerotium rolfsii at 40 μ g/mL [90]. …”

Section: Antifungal and Antiparasitic Activities Of Lectinsmentioning

confidence: 99%

Lectins, Interconnecting Proteins with Biotechnological/Pharmacological and Therapeutic Applications

Coelho

Silva

Lima

et al. 2017

Evidence-Based Complementary and Alternative Medicine

160

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Lectins are proteins extensively used in biomedical applications with property to recognize carbohydrates through carbohydrate-binding sites, which identify glycans attached to cell surfaces, glycoconjugates, or free sugars, detecting abnormal cells and biomarkers related to diseases. These lectin abilities promoted interesting results in experimental treatments of immunological diseases, wounds, and cancer. Lectins obtained from virus, microorganisms, algae, animals, and plants were reported as modulators and tool markers in vivo and in vitro; these molecules also play a role in the induction of mitosis and immune responses, contributing for resolution of infections and inflammations. Lectins revealed healing effect through induction of reepithelialization and cicatrization of wounds. Some lectins have been efficient agents against virus, fungi, bacteria, and helminths at low concentrations. Lectin-mediated bioadhesion has been an interesting characteristic for development of drug delivery systems. Lectin histochemistry and lectin-based biosensors are useful to detect transformed tissues and biomarkers related to disease occurrence; antitumor lectins reported are promising for cancer therapy. Here, we address lectins from distinct sources with some biological effect and biotechnological potential in the diagnosis and therapeutic of diseases, highlighting many advances in this growing field.

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Purification, Characterization, and Molecular Cloning of a Novel Antifungal Lectin From the Roots of Ophioglossum pedunculosum

Cited by 18 publications

References 43 publications

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Phytochemicals from fern species: potential for medicine applications

Lectins, Interconnecting Proteins with Biotechnological/Pharmacological and Therapeutic Applications

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