Purification, compositional analysis and antioxidant properties of polysaccharides from black ginseng

Abstract

Purpose: To extract, purify black ginseng polysaccharides, and study their compositional analysis and antioxidant properties. Methods: Crude polysaccharides from black ginseng were prepared by hot water extraction and subjected to chromatographic purification on columns to yield 4 polysaccharide components: . The BGPs were characterized by chemical analysis, gel permeation chromatography (GPC), gas chromatographymass spectrometry (GC-MS), and Fourier transform-infrared spectroscopy (FT-IR). Finally, the in vitro antioxidant activities of the BGPs were determined through their capacities to scavenge superoxide anion, as well as 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals. Results:The four fractions designated

Black Ginseng and Its Saponins: Preparation, Phytochemistry and Pharmacological Effects

“…Maltol inhibited Fe 2+ -stimulated DNA degradation by bleomycin with little prooxidant activity; it might act as a scavenger of free radicals as through its in vitro ability to inhibit erythrocyte membrane protein polymerization and membrane lipids oxidization after exposure to butyl hydroperoxide [146]. Four acidic polysaccharide fractions (BGP-60, BGP-65, BGP-70 and BGP-80) with estimated molecular weights of 28.6, 26.7, 11.4 and 3.05 kDa, respectively, were purified from black ginseng and showed strong potential antioxidant activities against DPPH, superoxide anion radicals and hydroxyl radical [147].…”

Comparisons of Isolation Methods, Structural Features, and Bioactivities of the Polysaccharides from Three Common Panax Species: A Review of Recent Progress

“…The proposed structure of a low-molecular weight polysaccharide of ginseng, MCGP-L, was clearly elucidated by monosaccharide composition and methylation analyses and NMR spectroscopy [ 55 ]. Furthermore, different ranges of molecular weight, various monosaccharide compositions, HG backbone with hairy regions of RG-I, α-dominating configurations, and (1→)- or (1→6)- and (1→3)-glycosidic linkages were found in other ginseng polysaccharides extracted by hot water [ 27 , 34 , 36 , 52 , 54 , 67 , 68 , 69 , 70 ]. It was determined that RG-I, xylan, and xyloglucan are major components in ginseng polysaccharides sequentially extracted by Na 2 CO 3 [ 37 ].…”