Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from<i>Bacillus subtilis</i>

Straaten, K.E. Van; Langill, David M.; Palmer, David R. J.; Sanders, D.A.R.

doi:10.1107/s1744309109009038

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…The aminosaccharide 3,3′-neotrehalosadiamine ( 70 ) isolated from the RNA polymerase-mutated B. subtilis 84R5 displayed moderate activity against S. aureus and Klebsiella pneumoniae as determined by an agar diffusion assay. , A putative aminotransferase from B. subtilis required for the biosynthesis of 70 has been isolated and characterized by crystallization . Beyond that, however, the biosynthetic pathway of 70 has not been thoroughly investigated and the mode of action is still unclear.…”

Section: Miscellaneous Compoundsmentioning

confidence: 99%

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Bioactive Secondary Metabolites from Bacillus subtilis: A Comprehensive Review

2019

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Bacillus subtilis is widely underappreciated for its inherent biosynthetic potential. This report comprehensively summarizes the known bioactive secondary metabolites from B. subtilis and highlights potential applications as plant pathogen control agents, drugs, and biosurfactants. B. subtilis is well known for the production of cyclic lipopeptides exhibiting strong surfactant and antimicrobial activities, such as surfactins, iturins, and fengycins. Several polyketide-derived macrolides as well as nonribosomal peptides, dihydroisocoumarins, and linear lipopeptides with antimicrobial properties have been reported, demonstrating the biosynthetic arsenal of this bacterium. Promising efforts toward the application of B. subtilis strains and their natural products in areas of agriculture and medicine are underway. However, industrial-scale availability of these compounds is currently limited by low fermentation yields and challenging accessibility via synthesis, necessitating the development of genetically engineered strains and optimized cultivation processes. We hope that this review will attract renewed interest in this often-overlooked bacterium and its impressive biosynthetic skill set.

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Section: Miscellaneous Compoundsmentioning

confidence: 99%

“…195,196 A putative aminotransferase from B. subtilis required for the biosynthesis of 70 has been isolated and characterized by crystallization. 197 Beyond that, however, the biosynthetic pathway of 70 has not been thoroughly investigated and the mode of action is still unclear.…”

Section: ■ Miscellaneous Compoundsmentioning

confidence: 99%

Bioactive Secondary Metabolites from Bacillus subtilis: A Comprehensive Review

2019

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“…We subcloned each of ntdA , ntdB , and ntdC into pET-28b and expressed them in E. coli BL21-Gold cells. The N-terminal hexahistidine-tagged proteins were purified by nickel-affinity chromatography.…”

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confidence: 99%

A Previously Unrecognized Kanosamine Biosynthesis Pathway in Bacillus subtilis

et al. 2013

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The ntd operon in Bacillus subtilis is essential for biosynthesis of 3,3'-neotrehalosadiamine (NTD), an unusual nonreducing disaccharide reported to have antibiotic properties. It has been proposed that the three enzymes encoded within this operon, NtdA, NtdB, and NtdC, constitute a complete set of enzymes required for NTD synthesis, although their functions have never been demonstrated in vitro. We now report that these enzymes catalyze the biosynthesis of kanosamine from glucose-6-phosphate: NtdC is a glucose-6-phosphate 3-dehydrogenase, NtdA is a pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase, and NtdB is a kanosamine-6-phosphate phosphatase. None of these enzymatic reactions have been reported before. This pathway represents an alternate route to the previously reported pathway from Amycolatopsis mediterranei which derives kanosamine from UDP-glucose.

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“…B. subtilis strain 84R5 produces aminosaccharide 3,3′-neotrehalosadiamine, which exhibits marginal activity against K. pneumoniea and S. aureus [ 207 ]. The biosynthetic pathway and mode of action of aminosacharide 3,3-neotrehalosadiamine are yet not elucidated; however, a putative aminotransferase, associated with its biosynthesis has been purified and characterized thoroughly [ 208 ].…”

Section: Miscellaneous Metabolitesmentioning

confidence: 99%

Classification and Multifaceted Potential of Secondary Metabolites Produced by Bacillus subtilis Group: A Comprehensive Review

et al. 2023

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Despite their remarkable biosynthetic potential, Bacillus subtilis have been widely overlooked. However, their capability to withstand harsh conditions (extreme temperature, Ultraviolet (UV) and γ-radiation, and dehydration) and the promiscuous metabolites they synthesize have created increased commercial interest in them as a therapeutic agent, a food preservative, and a plant-pathogen control agent. Nevertheless, the commercial-scale availability of these metabolites is constrained due to challenges in their accessibility via synthesis and low fermentation yields. In the context of this rising in interest, we comprehensively visualized the antimicrobial peptides produced by B. subtilis and highlighted their prospective applications in various industries. Moreover, we proposed and classified these metabolites produced by the B. subtilis group based on their biosynthetic pathways and chemical structures. The biosynthetic pathway, bioactivity, and chemical structure are discussed in detail for each class. We believe that this review will spark a renewed interest in the often disregarded B. subtilis and its remarkable biosynthetic capabilities.

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Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase fromBacillus subtilis

Cited by 5 publications

References 21 publications

Bioactive Secondary Metabolites from Bacillus subtilis: A Comprehensive Review

Bioactive Secondary Metabolites from Bacillus subtilis: A Comprehensive Review

A Previously Unrecognized Kanosamine Biosynthesis Pathway in Bacillus subtilis

Classification and Multifaceted Potential of Secondary Metabolites Produced by Bacillus subtilis Group: A Comprehensive Review

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