Purification, Identification and Functional Analysis of a Novel Immunomodulatory Peptide from Silkworm Pupa Protein

Effect of ultrasonic pretreatment on the emulsification properties of Clanis Bilineata Tingtauica Mell protein

“…CBTMP was extracted from CBTM by the method of Li et al [8] with subtle modifications. CBTM powders were defatted with petroleum ether at a flour ratio of 1:20 (w/v) for 12 h and for 3 times before protein extraction.…”

Silkworm Pupae: A Functional Food with Health Benefits for Humans

“…It has been reported that researchers have purified a novel immunomodulatory peptide with a molecular weight of 441.06 Da and an amino acid sequence of Asp-His-Ala-Val from silkworm pupae protein hydrolysate. This immunomodulatory peptide stimulated the expression of IL-6, IL-12, NF-κB, Cyclin D1, and cyclin-dependent kinase 4 [ 70 ]. Insects are an important source of active peptides, and their immunological effects on aquatic animals have been comprehensively reported, with insect consumption improving the immune status of aquatic animals [ 84 ].…”

Use of Alcalase in the production of bioactive peptides: A review

“…Splenocyte proliferation could be upgraded from 87.35% to 248.4% after induction by Concanavalin A, in the presence of 100 μg/ml of purified peptide [751]. In another research, a novel 441.06 Da immunomodulatory peptide was produced and isolated from ultrasound-pretreated silkworm (Bombyx mori) pupa protein after hydrolysis using Alcalase [752]. Splenic lymphocyte proliferation assay was used to test its pro-proliferative activity, and it was found that with 100 µg/mL of the purified peptide the splenocyte proliferation rate was 91.1% [752].…”

“…In another research, a novel 441.06 Da immunomodulatory peptide was produced and isolated from ultrasound-pretreated silkworm (Bombyx mori) pupa protein after hydrolysis using Alcalase [752]. Splenic lymphocyte proliferation assay was used to test its pro-proliferative activity, and it was found that with 100 µg/mL of the purified peptide the splenocyte proliferation rate was 91.1% [752]. In another work, the hydrolysates of ovalbumin, lysozyme and whole egg white produced by Alcalase hydrolysis were used to test the effects of the peptides produced on antibody production, cytokine secretion, oxidative stress and proliferation of murine spleen and mesenteric lymph node cells [753].…”