Purification of Extracellular Collagenase from Pseudomonas sp: Remarkable Collagenolytic Activity

Gautam, Manisha

doi:10.19080/aibm.2017.04.555633

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…The shift of optimum pH was attributed to the electrostatic interaction influenced by the carrier microenvironment. The results of this study was compatible with the results mentioned by Gautam et al (15), they found that the optimum pH of collagenase activity purified from S. aureus was 7.0. Effect of temperature on collagenase activity: The activity of partial pure collagenase was measured at temperatures ranging from 25°C to 60°C.…”

Section: Characterization Of Collagenase Effect Of Ph On Collagenase ...supporting

confidence: 93%

Assessement of Purified Collagenase Inhibition Activity From Staphyllococcus Aureus Hn77 by Some Local Plants Extract

Salih¹,

Hussein²

2022

Iraqi J. Agric. Sci.

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This study was aimed to inhibition of purified collagenase produced from S. aureus by local plants inhibitors (fruits and leaves). Sixty two local isolates of S. aureus which were isolated and identified by biochemical test subjected to the primary and secondary screening processes to select the active S. aureus isolate for collagenase production. Among these isolates, forty nine isolates with maximum zone of hydrolysis ability in primary screening (the ratio of Z/G more than 10 mm) were selected for secondary screening. It has been found that S. aureus HN77 had the highest productivity of the enzyme (49.122 U/mg protein). The selected isolate with highest level of collagenase activity was identified as S. aureus HN77 according to vitek test. The optimum conditions of collagenase production by selected isolate using submerged fermentation were obtained with medium (D) contain collagen as the best production medium, 2% starch as the best carbon source, 2% yeast extract/peptone as nitrogen source, temperature 37 °C and pH 7, after 48 hr. of incubation period, the specific activity was reached to1502.15 U/mg. The enzyme was purified by gel filtration chromatography using Sephadex G-150 then concentration by sucrose. The results shown an increase in final purification folds 6.1 time with an enzyme yield of 85.2%.The purified enzyme was exhibited maximal activity and stability at pH 7.0. The optimal temperature for purified enzyme activity was 45°C and it was stable until 45°C.The highest rate of enzyme specificity found with collagen. Collagenase was inhibited with local plants extract (fruit and leaves). Fruit of black fig extract inhibit 90% of collagenase activity while the inhibition efficiency of collagenase with grape leaf was reached to 12%.

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Section: Characterization Of Collagenase Effect Of Ph On Collagenase ...supporting

confidence: 93%

Assessement of Purified Collagenase Inhibition Activity From Staphyllococcus Aureus Hn77 by Some Local Plants Extract

Salih¹,

Hussein²

2022

Iraqi J. Agric. Sci.

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“…Moreover, Yasmin et al [1] reported that IAA producing PGPR enhanced the nutrients uptake through modulating the root architecture in the drought stressed soil. Thus, PGPB can serve as a phytostimulator but potential traits of the PGPB can be altered genetically by different environmental stresses [5]. Consortium of newly isolated and already characterized PGPR will ensure their sustainable use in crop production [6].…”

Section: Introductionmentioning

confidence: 99%

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

et al. 2020

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Salt stress is one of the devastating factors that hampers growth and productivity of soybean. Use of Pseudomonas pseudoalcaligenes to improve salt tolerance in soybean has not been thoroughly explored yet. Therefore, we observed the response of hydroponically grown soybean plants, inoculated with halotolerant P. pseudoalcaligenes (SRM-16) and Bacillus subtilis (SRM-3) under salt stress. In vitro testing of 44 bacterial isolates revealed that four isolates showed high salt tolerance. Among them, B. subtilis and P. pseudoalcaligenes showed ACC deaminase activity, siderophore and indole acetic acid (IAA) production and were selected for the current study. We determined that 10 6 cells/mL of B. subtilis and P. pseudoalcaligenes was sufficient to induce tolerance in soybean against salinity stress (100 mM NaCl) in hydroponics by enhancing plant biomass, relative water content and osmolytes. Upon exposure of salinity stress, P. pseudoalcaligenes inoculated soybean plants showed tolerance by the increased activities of defense related system such as ion transport, antioxidant enzymes, proline and MDA content in shoots and roots. The Na + concentration in the soybean plants was increased in the salt stress; while, bacterial priming significantly reduced the Na + concentration in the salt stressed soybean plants. However, the antagonistic results were observed for K + concentration. Additionally, soybean primed with P. pseudoalcaligenes and exposed to 100 mM NaCl showed a new protein band of 28 kDa suggesting that P. pseudoalcaligenes effectively reduced salt stress. Our results showed that salinity tolerance was more pronounced in P. pseudoalcaligenes as compared to B. subtilis. However, a detailed study at molecular level to interpret the mechanism by which P. pseudoalcaligenes alleviates salt stress in soybean plants need to be explored.

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“…As a result, under such conditions the bacterial cell membrane easily transports these ions into the cell and changes its osmotic pressure, which can affect the viability of the bacteria. Researchers have noticed that the activity of P. aeruginosa JCM5962 (T) enzymes and other species of Pseudomonas decreases when the medium has a high Na + ion concentration (Gautam and Azmi 2017;Sachan et al 2017). strategy of adding inoculum progressively was effective in improving the fermentation process.…”

Section: Resultsmentioning

confidence: 99%

Lactobionic acid production by Pseudomonas taetrolens in a fed‐batch bioreactor using acid whey as substrate

Sarenkova

Sáez‐Orviz

Ciproviča

et al. 2022

Int J of Dairy Tech

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Acid whey causes a major disposal problem for the dairy industry due to its low pH and high salt concentration. Lactobionic acid (LBA) production by whey fermentation is an inexpensive process. The goal of this work was to employ acid whey for LBA production. Acid whey was fermented in a bioreactor, adding Pseudomonas taetrolens LMG 2336 at 30°C and 6.5 pH, with 1.5-Lpm oxygen aeration and 350-rpm agitation. Three fermentation tests were carried out with a different amount of inoculum (10%, 30% and 10% three times within 24 h). Results indicated that repeatedly adding P. taetrolens inoculum to the acid whey substrate allows a complete lactose conversion into LBA, while the lactose oxidation process was insufficient in the sample where the inoculum was only added at the beginning of the fermentation process (only 29.7% LBA production with 10% inoculum). The physiological heterogeneity of P. taetrolens was determined by multiparametric flow cytometry, and results showed that there was a phenotypic adaptation of the microorganism due to the changes observed in its heterogeneity and physiological state. The results achieved will help to recycle acid whey for value-added product production such as LBA production.

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Purification of Extracellular Collagenase from Pseudomonas sp: Remarkable Collagenolytic Activity

Cited by 6 publications

References 21 publications

Assessement of Purified Collagenase Inhibition Activity From Staphyllococcus Aureus Hn77 by Some Local Plants Extract

Assessement of Purified Collagenase Inhibition Activity From Staphyllococcus Aureus Hn77 by Some Local Plants Extract

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

Lactobionic acid production by Pseudomonas taetrolens in a fed‐batch bioreactor using acid whey as substrate

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