Purification of lysozyme from chicken egg white using nanofiber membrane immobilized with Reactive Orange 4 dye

Multilayered Bio-Based Electrospun Membranes: A Potential Porous Media for Filtration Applications

“…Several ligands, proteins, and enzymes were immobilized on a broad range of biodegradable electrospun polymers (such as PVA, PGA, and PLA) depending on the requirement. An affinity membrane that simultaneously combines size-based filtration and high selectivity is now an attractive approach for purifying and filtering biological fluids (Fu et al, 2018;Ng et al, 2019).…”

Reduce, Reuse and Recycle in Protein Chromatography: Development of an Affinity Adsorbent from Waste Paper and Its Application for the Purification of Proteases from Fish By-Products

“…The triazine dye, CB3GA, is a well-established ligand in affinity chromatography [2,3,8,9,41]. The presence of hydrophobic, ionic and aromatic moieties in CB3GA give rise to the formation of mixed type interactions with proteins such as electrostatic, hydrophobic, hydrogen bonding interaction [11][12][13][20][21][22]43,44]. The presence of the chlorotriazine ring in CB3GA allows its direct immobilization onto the matrix.…”

“…In particular, the adsorption dynamic capacity of BSA was 1.4 and 1.8 mg/mL for CB3GA-Cellulose-1 and CB3GA-Cellulose-2, respectively, however the capacity for LYS was 1.5 and 3.3 mg/mL for CB3GA-Cellulose- Frontal analysis was employed to evaluate the dynamic capacity and chromatographic performance of the CB3GA-Cellulose-1 and CB3GA-Cellulose-2 affinity adsorbents, using two standard proteins (BSA and LYS) and the results are shown in Figure 3. BSA and LYS are two well-studied proteins, which are frequently used as models in protein chromatography [13,20,21]. They differ in molecular weight and isoelectric point (pI) and thus, provide useful conclusions concerning the chromatographic behavior of many adsorbents.…”

“…Purification methods that are designed based on specific, effective and robust materials are expected to guide the future of the protein purification area [6,7]. Affinity chromatography is the most specialized method for the effective purification of proteins, compared to other separation methods [2][3][4][8][9][10][11][12][13]. It offers high selectivity, resolution, and capacity in most protein purification procedures.…”

“…In the present study, we employed the triazine dye Cibacron Blue 3GA (CB3GA) as an immobilized ligand. Triazine dyes display several advantages compared to specific biological ligands due to their low cost, resistance to biological and chemical degradation and high protein binding capacity [10][11][12][13][18][19][20][21][22]. In addition, the presence of the triazine ring allows their direct immobilization to the matrix (e.g., Sepharose or cellulose) through a nucleophilic substitution reaction [15,16].…”