Purification of α1-proteinase inhibitor by triazine dye affinity chromatography, ion-exchange chromatography and gel filtration on fractogel TSK

Gunzer, Gerhard; Hennrich, Norbert

doi:10.1016/s0021-9673(01)96415-5

Cited by 28 publications

(5 citation statements)

References 12 publications

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“…Similar observation was also reported previously in the lipase adsorption process (Salis et al, 2005). In the affinity adsorption process of proteins, proteins without net charge at their pIs are beneficial to be adsorbed (Gunzer and Hennrich, 1984). The maximum laccase adsorption on MMSNPs-CPTS-IDA-Cu 2+ was observed at its pI of pH 3.5 among all pH values tested.…”

Section: Laccase Immobilization Parameterssupporting

confidence: 89%

Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

Wang

Chen

Yang

et al. 2010

Bioresource Technology

183

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Section: Laccase Immobilization Parameterssupporting

confidence: 89%

Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

Wang

Chen

Yang

et al. 2010

Bioresource Technology

183

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“…3. Proteins have no net charge at their isoelectric points, and therefore the maximum adsorption from aqueous solutions is usually observed at their isoelectric points 42. The isoelectric pH of laccase used in this study was 3.0.…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Pycnoporus sanguineus laccase by metal affinity adsorption on magnetic chelator particles

Wang

Chen

Liu

et al. 2007

J of Chemical Tech & Biotech

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BACKGROUND: Immobilized enzymes provide many advantages over free enzymes including repeated or continuous reuse, easy separation of the product from reaction media, easy recovery of the enzyme, and improvement in enzyme stability. In order to improve catalytic activity of laccase and increase its industrial application, there is great interest in developing novel technologies on laccase immobilization.

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“…The purification procedure consisted of affinity chromatography on Fractogel® TSK-Red, ion exchange chromatography on Fractogel® TSK DEAE-650, and gel filtration on Fractogel® TSK HW-55 according to I.e. (12). The resulting preparation of ar proteinase inhibitor was further purified by chromatography on Concanavalin A-Sepharose® (2.6 cm x 20 cm), previously equilibrated with 50 mmol/1 Tris/HCl buffer pH 7.5 containing l mmol/1 MgCh, l mmol MnCb, and l mmol/1 CaCb.…”

Section: Ai-proteinase> Inhibitor and Antibodiesmentioning

confidence: 99%

“PMN-Elastase Assay”: Enzyme Immunoassay for Human Polymorphonuclear Elastase Complexed with α1-Proteinase Inhibitor

Neumann¹,

Gunzer²,

Hennrich³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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Summary:A solid phase, enzyme-linkcd immunoassay is described for the quantitative determination of the complex of human granulocyte elastase (EC 3.4.21.37) with αι-proteinase inhibitor. The assay employs antibody-coated test tubes and it is suitable for routine use in clinical chemistry laboratories. Data for sample stability and test characteristics are given. A reference r nge of 20-180 μg/l elastase in plasma was determined. The diagnostic significance of granulocyte elastase levels in plasma in inflammatory diseases is discussed. "PMN-Elastase Assay": Enzymimmunassay zur Bestimmung des Komplexes von menschlicher GranulocytenElastase mit a/-ProteinaseinhibitorZusammenfassung: Es wird ein Festphasen-Enzymimmunoassay zur quantitativen Bestimmung des Komplexes aus menschlicher Granulocyten-Elastase und arProteinaseinhibitor beschrieben. Es handelt sich um eine routinef hige Testversion mit Antik rper-beschichteten R hrchen. Daten zur Stabilit t von Proben und die Testcharakteristica werden angegeben. Als Referenzbereich f r Elastase im Plasma wurden 20-180 μο/1 ermittelt. Die Bedeutung der Granulocyten-Elastase im Plasma f r die Diagnose und Verlaufskontrolle von entz ndlichen Prozessen wird diskutiert.

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Purification of α1-proteinase inhibitor by triazine dye affinity chromatography, ion-exchange chromatography and gel filtration on fractogel TSK

Cited by 28 publications

References 12 publications

Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

Immobilization of Pycnoporus sanguineus laccase by metal affinity adsorption on magnetic chelator particles

“PMN-Elastase Assay”: Enzyme Immunoassay for Human Polymorphonuclear Elastase Complexed with α1-Proteinase Inhibitor

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