Purification, sequencing and evaluation of a divergent phytase from &lt;i&gt;Penicillium oxalicum&lt;/i&gt; KCTC6440

Kim, Bong-Hyun; Lee, Ji Yeon; Lee, Peter Chang-Whan

doi:10.2323/jgam.61.117

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…The purified phytase enzyme activity was inhibited by these cations at both the concentration of 0.5mM and 1mM. (Table 5) and corroborates results with earlier studies (Kim et al, 2015). However, Shimizu (1992) have found that activity of extracellular phytase from B. subtilis (natto) N-77 strain was inhibited by the addition of EDTA, Fe 2+ , Zn 2+ , Ca 2+ , Ba 2+ , Cu 2+ ,and Al 3 suggesting that metal ions are not required for the functioning of enzyme as different organisms require different metal ions (Roy et al, 2012).…”

Section: Effect Of Metal Ions On Purified Phytase Enzyme Activitysupporting

confidence: 91%

Optimization, Purification and Characterization of Phytase from Isolated Probiotic Pediococcus acidilactici BNS5B

Sharma¹,

Shukla²

2019

Int.J.Curr.Microbiol.App.Sci

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Plant based diet such as vegetables, cereals, legumes and oilseeds contain 80% of total phosphorous in the form of phytic acid-cation complexes, bound phosphorous being excreted in manure due to unavailability of phytate degrader in the gastrointestinal tract of monogastric animals (Ashraf et al., 2013). The undegraded phytate leads to phosphorous deficiency in animals, elevated levels of phosphorous in soil and eutrophication of water bodies and renders phytic acid as the anti-nutritive factor by decreasing the bioavailability cations such as iron, calcium, magnesium, phosphorous, zinc, iodine, etc (Madsen, 2019;Singh et al., 2013). Most of these cations are involved in various physiological functions as their deficiency may lead to conditions such as anemia,

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Section: Effect Of Metal Ions On Purified Phytase Enzyme Activitysupporting

confidence: 91%