PUVA-induced Repigmentation of Vitiligo: Scanning Electron Microscopy of Hair Follicles

Ortonne, Jean‐Paul; Schmitt, Daniel; Thivolet, J

doi:10.1111/1523-1747.ep12514597

Cited by 125 publications

(77 citation statements)

References 5 publications

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“…6 Interestingly, RCM detected some of these melanocytes in hair follicles, the previously described follicular ''reservoir'' of vitiligo. 7 The present study does not indicate the origin of melanocytes for repigmentation process in vitiligo, but it suggests that a time-course RCM study during phototherapy of vitiligo lesions could clarify this question.…”

Section: Ausiliacontrasting

confidence: 61%

A noninvasive technique, reflectance confocal microscopy, for the characterization of melanocyte loss in untreated and treated vitiligo lesions

Kang

Duff

Passeron

et al. 2010

Journal of the American Academy of Dermatology

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Section: Ausiliacontrasting

confidence: 61%

A noninvasive technique, reflectance confocal microscopy, for the characterization of melanocyte loss in untreated and treated vitiligo lesions

Kang

Duff

Passeron

et al. 2010

Journal of the American Academy of Dermatology

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“…During the repigmentation of vitiliginous skin, it is thought that MPs become activated, migrate along the basement membrane under the ORS into depigmented skin and then differentiate into mature melanocytes. 2,7) The major structural proteins of the basement membrane zone (BMZ) of both normal skin and hair follicles are type IV collagen (CIV), laminin (LN) and entactin. Fibronectin (FN) present in the BMZ located under the cells of the ORS is not a normal epidermal BMZ component.…”

mentioning

confidence: 99%

Stem Cell Factor Combined with Matrix Proteins Regulates the Attachment and Migration of Melanocyte Precursors of Human Hair Follicles <i>in Vitro</i>

Wang

et al. 2013

Biological & Pharmaceutical Bulletin

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In conjunction with matrix proteins, stem cell factor (SCF) plays an important role in the migration of melanocyte precursors (MPs) derived from the mouse embryo. However, no studies have demonstrated an effect of SCF on human follicular MPs migration in vitro. In this report, first we demonstrate the immature state of the follicular MPs. Then cell attachment rate was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Standard 48-well chemotaxis chambers were used for a transfilter migration assay. F-actin was labeled by rhodamine-conjugated phalloidin, and then organization of the actin cytoskeleton was observed by confocal microscope. In the results, we directly show that MPs adhere more strongly to fibronectin (FN), laminin (LN) and type IV collagen (CIV) than to the negative control. SCF decreased the adhesion of MPs to FN and CIV. A chemotaxis analysis showed that FN and CIV have chemotactic effects on MPs. FN showed an obvious increase in chemotactic effects on MPs with SCF treatment comparing with the control group, but there were no significant changes in the levels of chemotaxis with CIV and LN when the cells were treated with SCF. SCF was chemotactic to MPs, and the presence of FN caused a statistically significant increase in MPs migration at various concentrations of SCF. Furthermore, we showed that SCF, in combination with FN, could induce an apparent increase in actin stress fiber formation in MPs. Our results indicate that SCF, in combination with matrix proteins and in particular with FN, regulates the movement of MPs by both altering cell attachment and increasing cell chemotaxis.Key words melanocyte precursor; stem cell factor; matrix protein; attachment; chemotaxis When vitiligo patients undergo repigmentation, they develop small pigmented islands that expand and coalesce to form normally pigmented skin.1) It has been demonstrated that these islands of pigmentation are derived from melanocyte precursors (MPs) in the outer root sheath (ORS) of hair follicles. 2)Although this phenomenon has been clinically well supported and MPs have been cultured successfully in vitro, little previous work has been performed to evaluate the migration capability of MPs. [3][4][5][6] When cells migrate, they synchronously attach and detach to various matrix proteins. During the repigmentation of vitiliginous skin, it is thought that MPs become activated, migrate along the basement membrane under the ORS into depigmented skin and then differentiate into mature melanocytes.2,7) The major structural proteins of the basement membrane zone (BMZ) of both normal skin and hair follicles are type IV collagen (CIV), laminin (LN) and entactin. Fibronectin (FN) present in the BMZ located under the cells of the ORS is not a normal epidermal BMZ component. It can only be identified in the epidermal BMZ during embryonic development.8,9) Therefore, there are some differences between epidermal melanocytes and MPs in the matrix proteins surrounding the BMZ. Previous authors have evaluated the impact o...

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“…41 Ortonne et al postulated the existence of a melanocyte reservoir, specifically located in the lower portion of human hair follicles and they proposed that repigmentation of vitiligo was derived from the melanocyte reservoir in the hair follicles. 42 Cui et al confirmed that during vitiligo repigmentation, the number of inactive melanocytes in the outer sheath of the hair follicle increases significantly and some active melanocytes appear in the outer root sheath, hair follicle orifices, and around the perifollicular epidermis. 43 Repigmentation in vitiligo usually begins in the perifollicular area in almost all the cases on treatment.…”

Section: Follicular Graftingmentioning

confidence: 94%

Surgical management of vitiligo - An approach to the patient

Manchanda

Bansal

Pandey

2013

Nepal J Dermatol Venereol & Leprol

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Vitiligo vulgaris is an acquired chronic pigmentary disorder with an average world wide incidence of approximately 1%. Clinical classification of vitiligo is based on the distribution and extension of lesions as localized, generalized, and universal. As vitiligo has great psychosocial implications especially in dark-skinned individuals, treatment should begin early. Currrent modalities of treatment include medical therapy, surgical modalities and laser. Medical treatment options include topical corticosteroids, calcineurin inhibitors, vitamin-D derivatives, phototherapy (ultraviolet A [UVA], narrowband UVB), photochemotherapy (psoralen plus UVA [PUVA]. Surgical methods used in the management of vitiligo gave cosmetically acceptable results in short periods. Successful treatment with various surgical techniques requires an appropriate selection of patients mainly focusing upon the stability of the clinical disease. The choice of the procedure always depends upon the age, the area involved, site and the cosmetic need of the patient. The nature of the procedure, expected outcome and the complications should be discussed with the patient in detail. This article mainly deals with various dermatosurgical approaches in brief available till date for the treatment of vitiligo.

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PUVA-induced Repigmentation of Vitiligo: Scanning Electron Microscopy of Hair Follicles

Cited by 125 publications

References 5 publications

A noninvasive technique, reflectance confocal microscopy, for the characterization of melanocyte loss in untreated and treated vitiligo lesions

A noninvasive technique, reflectance confocal microscopy, for the characterization of melanocyte loss in untreated and treated vitiligo lesions

Stem Cell Factor Combined with Matrix Proteins Regulates the Attachment and Migration of Melanocyte Precursors of Human Hair Follicles <i>in Vitro</i>

Surgical management of vitiligo - An approach to the patient

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