Pyridine Based Fluorescence Probe: Simultaneous Detection and Removal of Arsenate from Real Samples with Living Cell Imaging Properties

Abstract

Pyridine based fluorescence probe, DFPPIC and its functionalized Merrifield polymer has been synthesized, characterized and used as an arsenate selective fluorescence sensor. Arsenate induced fluorescence enhancement is attributed to inter-molecular H-bonding assisted CHEF process. The detection limit for arsenate is 0.001 μM, much below the WHO recommended tolerance level in drinking water. DFPPIC can detect intracellular arsenate in drinking water of Purbasthali, West Bengal, India efficiently. Graphical Abstract DFPPIC and its Merrifield conjugate polymer are used for selective determination and removal of arsenate from real drinking water samples of Purbasthali, a highly arsenic contaminated region of West Bengal, India. DFPPIC is very promising to imaging arsenate in living cells.

An ESIPT-based fluorescent probe for the detection of multiple analytes and a facile approach to discriminate between arsenate and pyrophosphate in water

“…The binding constant (4.81 × 10 4 M −1 ) and LOD (3.9 μM) values were calculated for arsenate detection via the emission method. [43][44][45] Further, the sensitivity of HJ-DNP toward PPi was also determined via systematic fluorescence and UV-vis absorbance titration. As shown in Fig.…”

Subcellular Partitioning of Arsenic Trioxide Revealed by Label-Free Imaging

“…Using two arsenic derivatives [biotin-conjugated versions of p -aminophenylarsine oxide (PAPAO) and 4,6-bis­(1,3,2-dithiarsolan-2-yl)-7-hydroxy-3 H -phenoxazin-3-one (ReAsH)], Zhang and colleagues proposed that ATO binds to a distinctive form of nuclear bodies . Fluorescent probes, for example, (4 E )-4-(2-hydroxybenzylideneamino)-1,2-dihydro-2,3-dimethyl-1-phenylpyrazol-5-one (APSAL) and 4-methyl-2,6-bis­(( E )-(pyridin-2-ylmethylimino)­methyl)­phenol (DFPPIC), have also been used to assess the distribution of arsenic within cells. , In those studies, however, one could never be certain that the distribution of the fluorescently labeled arsenic compounds accurately reflects the distribution of ATO in cells simply because the fluorescent tags themselves could potentially bind in a nonspecific manner to a variety of molecules and organelles in cells. Such interactions could create a misleading impression of ATO pharmacokinetics.…”

Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application

“…Most of the previously reported arsenic sensors [37][38][39][40][41] are based on Schiff-base ligands which suffer from hydrolytic decomposition in aqueous or mixed aqueous solvents. 38 However, it is well recognized that oximes are fairly stable both in organic and aqueous medium and seems to allow for a more effective sensor for such hazardous ions.…”