Pyruvate kinase and total protein are regulated differently during growth of P-815 mastocytoma cells

Ngo, Julie-Lani; Orlando, Robert A.; Ibsen, Kenneth H.

doi:10.1016/0003-9861(86)90546-1

Archives of Biochemistry and Biophysics

1986

DOI: 10.1016/0003-9861(86)90546-1

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Pyruvate kinase and total protein are regulated differently during growth of P-815 mastocytoma cells

Julie-Lani Ngo¹,

Robert A. Orlando²,

Kenneth H. Ibsen³

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Cited by 5 publications

(1 citation statement)

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“…Mast cell enzymes such as L-histidine decarboxylase and pyruvate kinase are essential in histamine formation and cell proliferation, respectively, and are regulated at the translational level in human (1,2). Most of the known physiological effects on translation are exerted at the level of polypeptide chain initiation (3).…”

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confidence: 99%

Eukaryotic Translation Initiation Factor-6 Enhances Histamine and IL-2 Production in Mast Cells

Oh¹,

Filler

2001

The Journal of Immunology

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Eukaryotic translation initiation factor (eIF)-6 is known to be important in ribosome biogenesis. Previously, we have discovered that eIF-6 mRNA is induced in lung in a murine model of asthma. We also found that there was enhanced eIF-6 expression in mast cells stimulated with PMA plus calcium ionophore. Therefore, we hypothesized that the induction of eIF-6 enhances the production of bioactive mediators by mast cells upon allergic stimulation. In the current study, we found that eIF-6 mRNA was rapidly induced in murine mast cells stimulated by FcεRI cross-linking, which is a major physiologic stimulant for mast cells. eIF-6 was also induced in human mast cells upon stimulation. The increase in eIF-6 gene expression in murine mast cells was blocked by therapeutic agents such as dexamethasone and cyclosporin A. To determine the location and function of eIF-6, murine mast cells were transfected with a construct that overexpressed enhanced green fluorescent protein-tagged eIF-6. These experiments demonstrated that eIF-6 was localized predominantly in the nucleolus of the mast cells. Also, overexpression of enhanced green fluorescent protein/eIF-6 enhanced the production of histamine and IL-2, but not IL-4 by stimulated murine mast cells. These results suggest that eIF-6 regulates the production of selected bioactive mediators in allergic diseases. This is the first demonstration of a biologic function of eIF-6 in mammalian cells.

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confidence: 99%

Eukaryotic Translation Initiation Factor-6 Enhances Histamine and IL-2 Production in Mast Cells

Oh¹,

Filler

2001

The Journal of Immunology

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Metabolic changes during cell growth inhibition by p27 overexpression

Carvalhal

Marcelino

Carrondo

2003

Applied Microbiology and Biotechnology

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Independent regulation of pyruvate kinase expression by cyclic AMP and prostaglandin F2α in mouse mastocytoma cells

Ngo

Ibsen

1989

Archives of Biochemistry and Biophysics

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Pyruvate kinase and total protein are regulated differently during growth of P-815 mastocytoma cells

Cited by 5 publications

References 28 publications

Eukaryotic Translation Initiation Factor-6 Enhances Histamine and IL-2 Production in Mast Cells

Eukaryotic Translation Initiation Factor-6 Enhances Histamine and IL-2 Production in Mast Cells

Metabolic changes during cell growth inhibition by p27 overexpression

Independent regulation of pyruvate kinase expression by cyclic AMP and prostaglandin F2α in mouse mastocytoma cells

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