Qindan-capsule inhibits proliferation of adventitial fibroblasts and collagen synthesis

Ren, Min; Zhang, Jidong; Wang, Bo; Liu, Ping; Jiang, Hong; Liu, Guilin; Yin, Huiqiu

doi:10.1016/j.jep.2010.03.004

Cited by 8 publications

(18 citation statements)

References 31 publications

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“…QC was prepared as previously described (Ren et al, 2010(Ren et al, , 2011. The composition of QC is presented in Table 1.…”

Section: Preparation Of Qcmentioning

confidence: 99%

“…Our previous experimental results showed that losartan can effectively reverse the biological activities induced by TGF-b1 in AFs (Ren et al, 2010(Ren et al, , 2011; therefore, we chose losartan as the positive control drug. Losartan was administered at 30 mg/kg d (equivalent to six times the clinical dosage) by stomach irrigation once per day.…”

Section: Preparation Of Drug-containing Serummentioning

confidence: 99%

“…Losartan was administered at 30 mg/kg d (equivalent to six times the clinical dosage) by stomach irrigation once per day. The drug-containing serum was prepared as previously described (Ren et al, 2010(Ren et al, , 2011. We used the same doses described previously.…”

Section: Preparation Of Drug-containing Serummentioning

confidence: 99%

“…Adventitial fibroblasts (AFs) were isolated from 14-week-old WKY rats and cultured as described previously (Ren et al, 2010). All experiments were performed with fibroblasts at passages 2-3.…”

Section: Cell Culturementioning

confidence: 99%

“…A previous study from our group showed that QC inhibited the AF proliferation induced by TGF-b1 through the TGF-b1/Smad signaling pathway (Ren et al, 2010). Although TGF-b is generally associated with the Smad signaling pathway, recent findings have indicated that cell-specific TGF-b activity may be stimulated by the activation of other signaling pathways that are parallel to Smad or participate in cross-talk with the Smad pathway (Hayashida et al, 2003).…”

Section: Introductionmentioning

confidence: 95%

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The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

Lu¹,

Liu²,

Yuan³

et al. 2013

Pharmaceutical Biology

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(2013) The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts, Pharmaceutical Biology, 51:6, 712-721, DOI: 10.3109/13880209.2013 Context: Qindan capsule (QC), a compound used in traditional Chinese medicine, has been used as an anti-hypertensive agent in clinical settings for years. Our previous studies have shown that QC can improve the morphological index of the artery, down-regulate the collagen volume fraction in the media and inhibit the transformation of smooth muscle cells. However, the detailed mechanisms underlying its effects require further investigation, which might provide more scientific evidence for the clinical treatment of hypertensive vascular remodeling (VR). Objective: We investigated the effects of QC-containing serum on the TGF-b1/ERK signaling pathway, cell proliferation, migration, the cell cycle, apoptosis and matrix metalloproteinase synthesis (MMPs) in rat aortic adventitial fibroblasts (AFs). Materials and methods: AFs were cultured through tissue explants in vitro. The levels of extracellular signal-regulated kinase 1/2 (ERK1/2), phospho-ERK1/2 (p-ERK1/2), connective tissue growth factor (CTGF), MMP2 and MMP9 expression were measured by western blotting and RT-PCR. The proliferation and migration of AFs were measured by MTT and transwell migration assays. Cell cycle progression and apoptosis in AFs were analyzed by flow cytometry. Results:The proliferation and migration rates of AFs treated with transforming growth factor b1 (TGF-b1) for 24 h were 2.4 AE 0.75 and 2.2 AE 0.06 times higher than those of untreated AFs, and increases in the expression of p-ERK1/2 (3.7 AE 0.15 times), CTGF (3.3 AE 0.24 times), MMP2 (5.7 AE 0.37 times) and MMP9 (5.4 AE 0.46 times) (p50.05) were observed. Treatment with QC-containing serum significantly down-regulated cell proliferation (1.9 AE 0.06 times), migration (1.6 AE 0.05 times) and the expression of p-ERK1/2 (1.3 AE 0.75 times), CTGF (1.8 AE 0.64 times), MMP2 (1.6 AE 0.65 times) and MMP9 (1.4 AE 0.46 times) (p50.05). We also found that QC-containing serum down-regulated the percentage of cells in the G1 phase by 1.6 AE 0.43 times and increased early-phase apoptosis by 2.3 AE 0.33 times (p50.05) in AFs. Conclusions: QC effectively inhibits the proliferation and migration of AFs and changes cell bioactivity and MMPs, possibly through the TGF-b/ERK/CTGF signaling pathway. Our findings may provide new insights into the potential function of QC in preventing or treating hypertension.

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“…QC was prepared as previously described (Ren et al, 2010(Ren et al, , 2011. The composition of QC is presented in Table 1.…”

Section: Preparation Of Qcmentioning

confidence: 99%

Section: Preparation Of Drug-containing Serummentioning

confidence: 99%

Section: Preparation Of Drug-containing Serummentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

Lu¹,

Liu²,

Yuan³

et al. 2013

Pharmaceutical Biology

Self Cite

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Qindan capsule (芩丹胶囊) changes adventitial collagen synthesis in spontaneously hypertensive rats

Liu

et al. 2013

Chin. J. Integr. Med.

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Banxia Baizhu Tianma Decoction for Essential Hypertension: A Systematic Review of Randomized Controlled Trials

Xiong

Yang

Liu

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Objectives. To assess the current clinical evidence of Banxia Baizhu Tianma Decoction (BBTD) for essential hypertension (EH). Search Strategy. Electronic databases were searched until July 2012. Inclusion Criteria. We included randomized clinical trials testing BBTD against placebo, antihypertensive drugs, or combined with antihypertensive drugs against antihypertensive drugs. Data Extraction and Analyses. Study selection, data extraction, quality assessment, and data analyses were conducted according to Cochrane standards. Results. 16 randomized trials were included. Methodological quality of the included trials was evaluated as generally low. 2 trials compared prescriptions based on BBTD using alone with antihypertensive drugs. Meta-analysis showed no significant effect of modified BBTD compared with captopril in systolic blood pressure (MD: −0.75 (−5.77, 4.27); P = 0.77) and diastolic blood pressure (MD: −0.75 (−2.89, 1.39); P = 0.49). 14 trials compared the combination of BBTD or modified BBTD plus antihypertensive drugs with antihypertensive drugs. Meta-analysis showed there are significant beneficial effect on systolic blood pressure in the combination group compare to the antihypertensive drugs (MD: −4.33 (−8.44, −0.22); P = 0.04). The safety of BBTD is uncertain. Conclusions. There is encouraging evidence of BBTD for lowering SBP, but evidence remains weak. Rigorously designed trials are warranted to confirm these results.

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Qindan-capsule inhibits proliferation of adventitial fibroblasts and collagen synthesis

Cited by 8 publications

References 31 publications

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

Qindan capsule (芩丹胶囊) changes adventitial collagen synthesis in spontaneously hypertensive rats

Banxia Baizhu Tianma Decoction for Essential Hypertension: A Systematic Review of Randomized Controlled Trials

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