Quadruple Response Factorial Design Optimization of Capillary Zone Electrophoresis Assay Procedure for Metformin and Sitagliptin Combination

Alnajjar, Ahmed O.; Idris, Abubakr M.; Attimarad, Mahesh; Elgorashe, Rafea E. E.

doi:10.1080/10826076.2015.1057646

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…Seven aliquots of MET and REM working standard solutions were transferred into 10 ml calibrated flasks, to obtain the amount of MET in the series of 2.5 to 30 µg/ ml (2.5, 5, 10, 15, 20, 25, 30) and REM in the range of 1 to 24 µg/ml (1,4,8,12,16,20, 24 µg/ml). UV absorption spectra were documented for these solutions in the range of 200 nm to 300 nm against dilute ethanol as solvent.…”

Section: Ratio Second Derivative Spectroscopic Methods (Rsds)mentioning

confidence: 99%

“…In addition, REM helps in reducing the body weight and blood pressure in diabetes mellitus patients. [8][9][10] Many analytical methods were illustrated for the estimation of MET from pharmaceutical preparations and plasma by spectroscopic, 11,12 HPLC, [13][14][15] HPLTC, 16 LC-MS, 17,18 and CZE, 19,20 alone and with other drugs. Few analytical procedures were also depicted in the texts for the analysis of REM.…”

Section: -Methyl-4-[[4-(1-methylethoxy)phenyl]methyl]-1-(1-mentioning

confidence: 99%

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Smart UV Derivative Spectrophotometric Methods for Simultaneous Determination of Metformin and Remogliflozin: Development, Validation and Application to the Formulation

Attimarad¹,

Nair²,

Nagaraja³

et al. 2023

Ind. J. Pharm. Edu. Res

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Background:The result of pharmaceutical industry research for the new class and the new combination of drugs for the treatments of diabetes is the newly approved combination of metformin (MET) and remogliflozin (REM). For the quality control of this formulation, three smart, reproducible and non-sophisticated spectroscopic techniques were developed by modification of UV spectra. Materials and Methods: The first two methods were based on the measurements of the peak height of the third derivative and second derivative ratio spectra of MET and REM and the third method was the constant center spectrum subtraction method. Results: The proposed methods exhibited Beer's law in the range of 2.5 to 30 µg/ml and 1 to 24 µg/ml for MET and REM correspondingly by all three methods. The mean percentage recovery was found to be in the range of 99.08% to 100.15% for MET and 98.73% to 100.27% for REM. Further, both analytes were quantified from the formulation using proposed spectroscopic methods with high accuracy. Comparison of all three methods with the reported HPLC method showed no variation in the assay outcomes in relation to accuracy and precision. Conclusion: The suggested techniques are simple, accurate and reproducible, hence could be used for regular quality control of formulation consisting of MET and REM.

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Section: Ratio Second Derivative Spectroscopic Methods (Rsds)mentioning

confidence: 99%

Section: -Methyl-4-[[4-(1-methylethoxy)phenyl]methyl]-1-(1-mentioning

confidence: 99%

Smart UV Derivative Spectrophotometric Methods for Simultaneous Determination of Metformin and Remogliflozin: Development, Validation and Application to the Formulation

Attimarad¹,

Nair²,

Nagaraja³

et al. 2023

Ind. J. Pharm. Edu. Res

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“…In another context, since its introduction of its modern version, capillary zone electrophoresis (CZE) has evolved into a highly mature analytical technique. After its instrument commercialization, CZE has taken its place among well-established analytical techniques, in general, and separation techniques, in particular [ 25 , 26 , 27 , 28 ]. This is because of its benefits in terms of versatility, fastness, very low consumption of reagents, and organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Development of Capillary Zone Electrophoresis Method for the Simultaneous Separation and Quantification of Metformin and Pioglitazone in Dosage Forms; and Comparison with HPLC Method

et al. 2023

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A capillary zone electrophoretic (CZE) method was developed, validated, and applied for the assay of metformin (MET) and pioglitazone (PIO) in pharmaceutical formulations. The optimum running buffer composition was found to be 75 mmol/L phosphate buffer containing 30% acetonitrile (ACN) at pH 4.0. The optimum instrumental conditions were found to be injection time, 10 s; applied voltage, 25 kV; hydrodynamic injection pressure, 0.5 psi for 10 sec, capillary temperature, 25 °C; and the detection wavelength, 210 nm. The quantifications were calculated based on the ratio of the peak areas of analytes to atenolol as an internal standard. The CZE method was validated in terms of accuracy (98.21–104.81%), intra- and inter-day precision of migration time and peak area (relative standard deviation ≤ 5%), linearity (correlation coefficients ≥ 0.9985), limit of detection (≤ 0.277 μg/mL), and limit of quantitation (≤ 0.315 μg/mL). The proposed method was applied for the analysis of PIO and MET both individually and in a combined dosage tablet formulation. All electrophoretic parameters were calculated and evaluated. A previously reported high-performance liquid chromatographic (HPLC) method was also applied to the same samples. A comprehensive comparison was then carried out for the analytical features of both methods CZE and HPLC. Comparable results were obtained with the advantage of reagent consumption and separation efficiency of CZE over HPLC and shorter analysis time by HPLC compared with CZE.

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“…Several methods are illustrated in the literature for the analysis of metformin alone and along with different classes of agents from formulations and body fluids. Recent methods reported were UV-spectrophotometry [ 19 , 20 , 21 ], RP-HPLC [ 22 , 23 , 24 , 25 ], HPTLC [ 24 , 25 ], capillary electrophoresis [ 26 , 27 ] and LCMS [ 28 , 29 , 30 , 31 , 32 ]. There were few reports in the literature for the simultaneous estimation of metformin along with SGLT-2 inhibitors [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness

Attimarad

Nair

Sreeharsha

et al. 2021

IJERPH

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The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were based on the peak amplitude difference and first-order derivative absorption of the ratio spectra developed by the manipulation of scanned UV spectra. REM and MET showed good linearity in the series of 1–20 µg ml−1 and 2.5–35 µg ml−1, respectively, by all three methods with an excellent correlation coefficient (r2 ≥ 0.998). Further, the proposed UV spectroscopic techniques were validated as per International Council for Harmonization guidelines. The methods showed good sensitivity, accuracy, and precision. Anticipated procedures were effectively utilized for the concurrent quantification of REM and MET in laboratory prepared mixtures and tablets. The high percent recovery with low standard deviation found for both analytes by all three methods confirms the accuracy and precision of the procedures. Finally, the greenness of the proposed spectroscopic methods, evaluated by semi-quantitative and quantitative methods, showed the eco-friendly nature of the methods. Furthermore, the proposed approaches were simple, accurate, sensitive, economic, and environmentally friendly and hence can be utilized for regular quality control of REM and MET formulation.

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Quadruple Response Factorial Design Optimization of Capillary Zone Electrophoresis Assay Procedure for Metformin and Sitagliptin Combination

Cited by 8 publications

References 19 publications

Smart UV Derivative Spectrophotometric Methods for Simultaneous Determination of Metformin and Remogliflozin: Development, Validation and Application to the Formulation

Smart UV Derivative Spectrophotometric Methods for Simultaneous Determination of Metformin and Remogliflozin: Development, Validation and Application to the Formulation

Development of Capillary Zone Electrophoresis Method for the Simultaneous Separation and Quantification of Metformin and Pioglitazone in Dosage Forms; and Comparison with HPLC Method

Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness

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