Qualitative analysis of Psoraleae Fructus by HPLC‐DAD/TOF‐MS fingerprint and quantitative analysis of multiple components by single marker

Abstract

A variety of bioactive substances may account for the recognized efficacy and wide clinical application of Psoraleae Fructus in China. A high-performance liquid chromatography-diode array detector (HPLC-DAD) fingerprint method was developed to present the comprehensive phytochemical profile of the crude drug. Thirteen major compounds were separated and identified by HPLC coupled with time-of-flight mass spectrometry (HPLC/TOF-MS), namely psoralenoside (PO), isopsoralenoside (IPO), psoralen (PS), isopsoralen (IPS), neobavaisoflavone (NBF), bavachin (BC), corylin (CN), bavachromene (BCM), psoralidin (PD), isobavachalcone (IBC), bacachinin (BCN), corylifol A (CA) and bakuchiol (BK). Then quantitative analysis of multiple components by single marker (QAMS) was applied in content determination of PO, IPO, PS, IPS, BC, IBC, BCN, CA and BK, with NBF as the internal standard. The calculation results indicated no significant difference from the traditional external standard method (p > 0.05, RSD < 2.62%), suggesting that QAMS is a reliable and convenient method for content determination of multiple chemical compositions, especially when there is a shortage of reference substances. In conclusion, simultaneous qualitative and quantitative analysis of Psoraleae Fructus may be fulfilled through the newly proposed method of QAMS combined with HPLC-DAD/TOF-MS fingerprint.

Analysis of the chemical components of pomelo peels (Citrus grandis [L.] Osbeck) from different cultivars by using supercritical CO2 fluid extraction and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry

“…Compound 34 showed a protonated molecular ion at m/z 187.0393 at 10.77 min, and the MS/MS spectrum generated fragment ions, including m/z 159.0439 [M+H‐CO] + , 143.0489 [M+H‐CO 2 ] + , 131.04912 [M+H‐2CO] + and 115.0543 [M+H‐CO 2 ‐CO] + . By comparing the obtained mass spectra with the previous literature, compound 34 was tentatively assigned to be isopsoralen [22]. The proposed fragmentation schemes are shown in Figure 3B.…”

Rapid characterization of chemical constituents and metabolites of Qi‐Jing‐Sheng‐Bai granule by using UHPLC–Q‐TOF‐MS

“…The other 23 compounds were characterized as isoflavones, chalcones, and flavonones. Ten of them (compounds 44 , 45 , 71 , 79 , 93 , 103 , 110 , 118 , 127 , and 133 ) were confirmed by comparing with standard compounds and the left constituents (compounds 25 , 29 , 35 , 68 , 103 , 108 , 117 , 98 , 131 , 33 , 52 , 127 , and 136 ) were tentatively identified by comparing with their accurate mass measurements of both MS spectra within the mass error ± 5 ppm and literature .…”

Screening the Marker Components in Psoralea corylifolia L. with the Aids of Spectrum-Effect Relationship and Component Knock-Out by UPLC-MS2

“…The HPLC four stage rod-electrostatic field orbit trap higher solution mass spectrometry analysis results of knocked-out components were shown in Figure 4 . Protonated ions, adducted ions, and fragment ions of each peak were then elucidated in accordance with reference HPLC chromatogram of standard solutions and related data in the literatures [ 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. The formula of compound P2 with [M + H] + ions at m / z 254, C 15 H 10 O 4 , produced ion fragments of 136/118, and were tentatively characterized as daidzein ( Figure 5 a).…”