Qualitative and quantitative evaluation of avian demineralized bone matrix in heterotopic beds

Sanaei, M. Reza; Abu, Jalila; Nazari, Mojgan; Zuki, A. B. Mohd; Allaudin, Zeenathul Nazariah

doi:10.1111/j.1532-950x.2013.12057.x

Cited by 3 publications

(1 citation statement)

References 48 publications

(89 reference statements)

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“…Процесс выделения костного матрикса из ксеногенной костной ткани обладает высокой технологичностью [19,20]; кроме того, за счет своей волокнисто-пористой структуры выделяемый кристаллический костный минерал является проницаемым для лекарственных средств, после имплантации успешно заселяется клетками и обладает высокой биосовместимостью, что делает его подходящей системой доставки лекарственных средств для таргетной терапии [21][22][23]. Еще одним важным достоинством костного матрикса при замещении костной ткани является его низкая иммуногенность, обусловленная естественной природой гидроксиапатита и высокой консервативностью белков экстрацеллюлярного матрикса в процессе эволюции [24]. Костный матрикс используется как в сегментарном виде для получения каркасов (матриксов, скаффолдов) [25], так и в виде микроразмерных или наноразмерных гранул для самостоятельного замещения костной ткани [26,27] либо в составе композитных материалов как вспомогательный материал с целью увеличения остео-кондуктивных и остеоиндуктивных свойств каркасов для замещения костных дефектов [28,29].…”

Section: ® Original Researchunclassified

Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects

Веремеев

Болгарин²,

Нестеренко

et al. 2020

Fundamentalʹnaâ i kliničeskaâ medicina

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Aim. To evaluate the efficacy of heterologous demineralised bone matrix (DBM) for the replacement of bone defects using a critical-sized rat calvarial defect model. Materials and Methods. For the experiments, we used 48 Sprague-Dawley rats (4.5 to 6 months of age). Critical-sized (8 mm diameter) calvarial defect was filled by the bone autograft, heterologous DBM, or comparator product (Geistlich BioOss®) or remained unfilled (negative control). Upon 4 or 12 weeks, rats were euthanised with the subsequent investigation of the defect and adjacent tissues by means of hematoxylin and eosin staining (mineralized tissue area to the defect area ratio) and microcomputed tomography (volume, thickness, and mineral density of the repaired tissue). Results. In our experimental setting, bone autograft was the most efficient in bone repair. Heterologous DBM and comparator product were equally efficient in filling the defect and did not show any statistically significant differences regarding any of the parameters. Microcomputed tomography and routine histological examination demonstrated concordant results. Conclusion. Heterologous DBM is efficient for the repair of critical-sized rat calvarial defects.

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Section: ® Original Researchunclassified

Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects

Веремеев

Болгарин²,

Нестеренко

et al. 2020

Fundamentalʹnaâ i kliničeskaâ medicina

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Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting

Harvanová

Horňák²,

Amrichová³

et al. 2014

Vet Res Commun

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Avian osteoblasts have been isolated particularly from chicken embryo, but data about other functional tissue sources of adult avian osteoblast precursors are missing. The method of preparation of pigeon osteoblasts is described in this study. We demonstrate that pigeon cancellous bone derived osteoblasts have particular proliferative capacity in vitro in comparison to mammalian species and developed endogenous ALP. Calcium deposits formation in vitro was confirmed by alizarin red staining. Only a few studies have attempted to investigate bone grafting and treatment of bone loss in birds. Lack of autologous bone grafts in birds has prompted investigation into the use of avian xenografts for bone augmentation. Here we present a method of xenografting of ostrich demineralised cancellous bone scaffold seeded with allogeneic adult pigeon osteoblasts. Ostrich demineralised cancellous bone scaffold supported proliferation of pigeon osteoblasts during two weeks of co - cultivation in vitro. Scanning electron microscopy demonstrated homogeneous adult pigeon osteoblasts attachment and distribution on the surface of xenogeneic ostrich demineralised cancellous bone. Our preliminary in vitro results indicate that demineralised cancellous bone from ostrich tibia could provide an effective biological support for growth and proliferation of allogeneic osteoblasts derived from cancellous bone of pigeons.

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Evaluation of Osteogenic Potentials of Avian Demineralized Bone Matrix in the Healing of Osseous Defects in Pigeons

et al. 2015

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Qualitative and quantitative evaluation of avian demineralized bone matrix in heterotopic beds

Abstract: Avian DBM initiates heterotopic bone formation in allogeneic recipients with low grades of immunogenicity. Implant geometry affects this phenomenon as osteoconduction appeared to augment the magnitude of the effects in larger tubular implants.

Cited by 3 publications

References 48 publications

Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects

Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects

Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting

Evaluation of Osteogenic Potentials of Avian Demineralized Bone Matrix in the Healing of Osseous Defects in Pigeons

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