Qualities of goat semen in Tris-Citrate-Glucose extender containing glutathione

Abstract

Glutathione (GSH) 0 (control), 2, 4 and 8 mM was used in the preservation of chilled goat semen. Treated and control samples were kept at 4 -5°C up to seven days. Sperm motility and acrosome abnormality were assessed daily under phase contrast microscope. The sperm motility was significantly (P<0.01) higher in the semen treated with 8 mM GSH. Optimum sperm motility (≥50%) for artificial insemination was retained for three days with 2 and 4 mM GSH and up to four days with 8 mM GSH. Acrosomal damage was significantly (P<0.01) reduced to ≤ 1.0% after addition of 8 mM GSH. It is suggested that GSH may be used as an antioxidant for better preservation of goat semen for artificial insemination. (Bangl. vet.

Seminal plasma contains TGF‐β and CXCL10 but sperm washing before cryopreservation is beneficial for post‐thawing sperm motility in Black Bengal goats (Capra hircus)

“…Attributes of semen especially ejaculate volume with good concentration, and motility of spermatozoa is important to create opportunity of producing maximum number of AI doses from an ejaculate. The volume of ejaculate was reported low and was varied between Black Bengal bucks, 2‐4 which were in agreement with the current investigation. The concentrations of spermatozoa in black Bengal bucks reported by other authors 3,29,30 were similar with the current estimation.…”

“…The concentrations of spermatozoa in black Bengal bucks reported by other authors 3,29,30 were similar with the current estimation. However, Rajuana et al 31 reported higher concentration of spermatozoa, while Mishra et al 4 reported very low concentration of spermatozoa in Black Bengal bucks compared to the current investigation. The variation could be due to the differences in environment and the differences of techniques and semen collectors 32,33 .…”

“…The semen was collected using artificial vagina (AV) measuring an outer casing (15 cm × 5.5 cm). The assembling of AV and maintaining of watertight jacket temperature are described elsewhere 4 . In order to obtain maximum quantity of good quality semen, the donor buck was adequately prepared and stimulated sexually.…”

“…The concentration of spermatozoa was calculated using a hemocytometer as described by Mishra et al 4 In brief, semen samples were diluted with 3% normal saline water (1:200). A volume of 10 µL was placed on each counting chamber and was incubated at 37°C for 5 minutes to settle down the spermatozoa on the counting chamber before placing them on the stage of the microscope.…”

“…Bucks usually discharge a small volume of semen with high concentrations of spermatozoa compared to bulls and Boars 1 . Black Bengal bucks discharge very small volume of ejaculate 2‐4 compared to other goat breeds 5,6 . Using artificial insemination with selected breeding bucks with good seminal attributes is required to improve the efficiency of AI and to improve the species 7 .…”