Quality Evaluation on Paeoniae Radix. I. Quantitative Analysis of Monoterpene Glycosides Constituents of Paeoniae Radix by Means of High Performance Liquid Chromatography. Comparative Characterization of the External Figures, Processing Method and the Cultivated Areas

IKEDA, Norihiro; FUKUDA, Tomomi; JYO, Hisanobu; SHIMADA, Yasuo; Murakami, Nobutoshi; Saka, M.; Yoshikawa, Masayuki

doi:10.1248/yakushi1947.116.2_138

Cited by 18 publications

(8 citation statements)

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“…were provided from Jungwoo Pharmaceutical Co (Asan, Korea) and authenticated by Dr. Youngbae Suh, College of Pharmacy, Seoul National University. Paeoniflorin, benzoylpaeoniflorin, albiflorin, glycyrrhizin, isoliquiritin and liquiritigenin were isolated according to the procedure as previously described (Tan et al, 1995;Ikeda et al, 1996;Song et al, 2004b). RAW 264.7 cells, murine macrophages, were obtained from the American Type Culture Collection (Rockville, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

Orthogonal array design for optimizing extraction efficiency of active constituents from Jakyak-Gamcho Decoction, the complex formula of herbal medicines, Paeoniae Radix and Glycyrrhizae Radix

Guo

Cho

Kang

et al. 2007

Journal of Ethnopharmacology

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Section: Methodsmentioning

confidence: 99%

Orthogonal array design for optimizing extraction efficiency of active constituents from Jakyak-Gamcho Decoction, the complex formula of herbal medicines, Paeoniae Radix and Glycyrrhizae Radix

Guo

Cho

Kang

et al. 2007

Journal of Ethnopharmacology

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“…Paeoniflorin (PF) is the main active component isolated from the herbal medicine Paeoniae Radix [1] . PF has many biological activities and has been shown to be anti-inflammatory [2][3][4] , analgesic [5] , and an antioxidant [6,7] .…”

Section: Introductionmentioning

confidence: 99%

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Zhong

Song

et al. 2015

Acta Pharmacol Sin

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Aim: Paeoniflorin from Chinese herb Paeoniae Radix has been shown to ameliorate middle cerebral artery occlusion-induced ischemia in rats. The aim of this study was to investigate the mechanisms underlying the neuroprotective action of PF in cultured rat cortical neurons. Methods: Primary cultured cortical neurons of rats were subjected to oxygen-glucose deprivation and reoxygenation (OGD/R) insult. Cell survival was determined using MTT assay. HEK293 cells stably transfected with A 1 R (HEK293/A 1 R) were used for detailed analysis. Phosphorylation of the signaling proteins was evaluated by Western blot or immunoprecipitation. Receptor interactions were identified using co-immunoprecipitation and immunofluorescence staining. Results: Paeoniflorin (10 nmol/L to 1 μmol/L) increased the survival of neurons subjected to OGD/R. Furthermore, paeoniflorin increased the phosphorylation of Akt and ERK1/2 in these neurons. These effects were blocked by PI3K inhibitor wortmannin or MEK inhibitor U0126. Paeoniflorin also increased the phosphorylation of Akt and ERK1/2 in HEK293/A 1 R cells. Both A 1 R antagonist DPCPX and EGFR inhibitor AG1478 not only blocked paeoniflorin-induced phosphorylation of ERK1/2 and Akt in HEK293/A 1 R cells, but also paeoniflorin-increased survival of neurons subjected to OGD/R. In addition, paeoniflorin increased the phosphorylation of Src kinase and activation of MMP-2 in HEK293/A 1 R cells. Both Src inhibitor PP2 and MMP-2/MMP-9 inhibitor BiPs not only blocked paeoniflorininduced phosphorylation of ERK1/2 (and Akt) in HEK293/A 1 R cells, but also paeoniflorin-increased survival of neurons subjected to OGD/R. Conclusion: Paeoniflorin promotes the survival of cultured cortical neurons by increasing Akt and ERK1/2 phosphorylation via A 1 Rmediated transactivation of EGFR.

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“…Pharmacological investigation showed that paeoniflorin was one of the major active principles of Si-Wu decoction [13]. Albiflorin, as well as paeoniflorin, are the characteristic constituents of R. paeoniae and used as markers to evaluate the quality of R. paeoniae [14,15]. The metabolic process of albiflorin by human intestinal bacteria was similar to that of paeoniflorin [16].…”

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction–liquid chromatographic method for the determination and pharmacokinetic studies of albiflorin and paeoniflorin in rat serum after oral administration of Si–Wu decoction

Sheng

Wang

et al. 2004

Journal of Chromatography B

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Quality Evaluation on Paeoniae Radix. I. Quantitative Analysis of Monoterpene Glycosides Constituents of Paeoniae Radix by Means of High Performance Liquid Chromatography. Comparative Characterization of the External Figures, Processing Method and the Cultivated Areas

Cited by 18 publications

References 0 publications

Orthogonal array design for optimizing extraction efficiency of active constituents from Jakyak-Gamcho Decoction, the complex formula of herbal medicines, Paeoniae Radix and Glycyrrhizae Radix

Orthogonal array design for optimizing extraction efficiency of active constituents from Jakyak-Gamcho Decoction, the complex formula of herbal medicines, Paeoniae Radix and Glycyrrhizae Radix

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Solid-phase extraction–liquid chromatographic method for the determination and pharmacokinetic studies of albiflorin and paeoniflorin in rat serum after oral administration of Si–Wu decoction

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