Quantification of acetaminophen and two of its metabolites in human plasma by ultra-high performance liquid chromatography–low and high resolution tandem mass spectrometry

Tonoli, David; Varesio, Emmanuel; Hopfgartner, Gérard

doi:10.1016/j.jchromb.2012.07.009

Cited by 47 publications

(20 citation statements)

References 43 publications

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“…The same mass transition has also been employed for quantification of non-protein-bound APAP-Cys in human plasma using triple quadrupole linear ion trap MS detection [43]. Method 2 incorporated an additional provision for APAPCys identification through inclusion of an analyte qualification transition (271.0 → 96.0 m/z).…”

Section: Methods Developmentmentioning

confidence: 99%

Quantification of a biomarker of acetaminophen protein adducts in human serum by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry: Clinical and animal model applications

Cook

King

Chang

et al. 2015

Journal of Chromatography B

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Section: Methods Developmentmentioning

confidence: 99%

Quantification of a biomarker of acetaminophen protein adducts in human serum by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry: Clinical and animal model applications

Cook

King

Chang

et al. 2015

Journal of Chromatography B

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“…As a matter of fact, the application of combined QUAL/QUAN strategies using DIA methods and high-resolution MS has produced a paradigm shift in the drug metabolism and pharmacokinetics (DMPK) field. 4-6 In proteomic studies, DIA is now established and provides the advantage of monitoring all detectable peptides with high sensitivity and reproducibility across large sample sets. 7 In other words, DIA methods combine the high-throughput from DDA with the high reproducibility of SRM.…”

Section: Introductionmentioning

confidence: 99%

Ranking Fragment Ions Based on Outlier Detection for Improved Label-Free Quantification in Data-Independent Acquisition LC–MS/MS

et al. 2015

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Data-independent acquisition LC-MS/MS techniques complement supervised methods for peptide quantification. However, due to the wide precursor isolation windows, these techniques are prone to interference at the fragment ion level, which in turn is detrimental for accurate quantification. The “non-outlier fragment ion” (NOFI) ranking algorithm has been developed to assign low priority to fragment ions affected by interference. By using the optimal subset of high priority fragment ions these interfered fragment ions are effectively excluded from quantification. NOFI represents each fragment ion as a vector of four dimensions related to chromatographic and MS fragmentation attributes and applies multivariate outlier detection techniques. Benchmarking conducted on a well-defined quantitative dataset (i.e. the SWATH Gold Standard), indicates that NOFI on average is able to accurately quantify 11-25% more peptides than the commonly used Top-N library intensity ranking method. The sum of the area of the Top3-5 NOFIs produces similar coefficients of variation as compared to the library intensity method but with more accurate quantification results. On a biologically relevant human dendritic cell digest dataset, NOFI properly assigns low priority ranks to 85% of annotated interferences, resulting in sensitivity values between 0.92 and 0.80 against 0.76 for the Spectronaut interference detection algorithm.

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“…This has triggered the publication of several multi-analyte LC-MS/MS assays, such as the study described by Tonoli et al [22] that can simultaneously quantify acetaminophen and its major conjugated metabolites. Our goal was to develop a highly sensitive assay that can be used for small volume, minimally invasive DBS sampling strategies for pediatric pharmacokinetic studies.…”

Section: Discussionmentioning

confidence: 99%

“…The sensitivity and range of reliable response of the present assay compared favorably with recently published LC-MS/MS assays. The assay described in reference [22] had a range of reliable response from 20-10,000 ng/ml in plasma and a recently described DBS LC-MS/MS assay [23] had a range of reliable response from 50-5,000 ng/ml.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography–tandem mass spectrometry

Taylor

Hoffman

Schniedewind

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

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Acetaminophen (paracetamol, N-(4-hydroxyphenyl) acetamide) is one of the most commonly prescribed drugs for the management of pain in children. Quantification of acetaminophen in pre-term and term neonates and small children requires the availability of highly sensitive assays in small volume blood samples. We developed and validated an LC-MS/MS assay for the quantification of acetaminophen in human plasma, cerebro-spinal fluid (CSF) and dried blood spots (DBS). Reconstitution in water (DBS only) and addition of a protein precipitation solution containing the deuterated internal standard were the only manual steps. Extracted samples were analyzed on a Kinetex 2.6 μm PFP column using an acetonitrile/formic acid gradient. The analytes were detected in the positive multiple reaction mode. Alternatively, DBS were automatically processed using direct desorption in a sample card and preparation (SCAP) robotic autosampler in combination with online extraction. The range of reliable response in plasma and CSF was 3.05-20,000 ng/ml (r2 > 0.99) and 27.4-20,000 ng/ml (r2 > 0.99) for DBS (manual extraction and automated direct desorption). Inter-day accuracy was always within 85-115% and inter-day precision for plasma, CSF and manually extracted DBS were less than 15%. Deming regression analysis comparing 167 matching pairs of plasma and DBS samples showed a correlation coefficient of 0.98. Bland Altman analysis indicated a 26.6% positive bias in DBS, most likely reflecting the blood: plasma distribution ratio of acetaminophen. DBS are a valid matrix for acetaminophen pharmacokinetic studies.

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Quantification of acetaminophen and two of its metabolites in human plasma by ultra-high performance liquid chromatography–low and high resolution tandem mass spectrometry

Cited by 47 publications

References 43 publications

Quantification of a biomarker of acetaminophen protein adducts in human serum by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry: Clinical and animal model applications

Quantification of a biomarker of acetaminophen protein adducts in human serum by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry: Clinical and animal model applications

Ranking Fragment Ions Based on Outlier Detection for Improved Label-Free Quantification in Data-Independent Acquisition LC–MS/MS

Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography–tandem mass spectrometry

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