Quantification of age pigments (lipofuscin)

Hammer, Cornelius; Braum, E.

doi:10.1016/0305-0491(88)90030-2

Cited by 33 publications

(22 citation statements)

References 161 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These wavelength ranges match well with those reported in the literature for LF (See review by Hammer & Braum 1988), particularly the data reported for the spider crab Hyas araneus (Hirche & Anger 1987), and for the nematode Caenorhabditis elegans (Davis et al 1982). A second emission maximum at 510 to 520 nm (at excitation wavelength of 340 to 350 nm) was found with extracted LF in eye-stalks.…”

Section: Methodological Refinementssupporting

confidence: 77%

Use of extractable lipofuscin for age determination of blue crab Callinectes sapidus

Ju¹,

Secor²,

Harvey³

1999

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

The blue crab Calhectes sapjdus is an econornicaily and ecologicdly irnportant species in rnany ternperate estuanes. yet stock assessments have been limited to length-based methods for demographic analyses. We evaluated the potential of age pigrnents (iipofuscins) sequestered in neural tissue of eye-stalks and brains to estimate the age of blue crabs coilected frorn Chesapeake Bay and Chincoteague Bay. The rate of lipofuscin accumulation was determined using crabs of known age reared in the laboratory. Age pigments were extracted from neural tissues (eye-stalk or brain). quantified, and normalized to protein content to al.low cornpansons across tissue types and crab sizes. Fieldcollected blue crabs (35 to 185 mm carapace width) contained highly variable levels of age pigments (coefficient of variation = 58%). Lipofuscin level was significantly related to carapace width, but not significantly different between gender or sarnpling location. In luveniles (40 to 70 mnl carapace width) reared for 6 mo, the age pigments showed no significant change during the rapid surnrner growth period, but significantly increased dunng fall (after 3 mo). Lipofuscin contents in known-age reared crabs were positively related to chronological age. Modal analysis of lipofuscin for field-coiiected adult males provided separation of multiple modes, whereas carapace width showed only a single broad rnode. These results confirm the potential use of Lipofuscin for age estimation of blue crabs.

show abstract

Section: Methodological Refinementssupporting

confidence: 77%

Use of extractable lipofuscin for age determination of blue crab Callinectes sapidus

Ju¹,

Secor²,

Harvey³

1999

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…Only a few findings are reported from clams so far: In the alimentary tract epithelium, in gonadal connective tissue and in the renal epithelium of Mya arenaria and Mercenaria mercenaria (Brown and O'Toole, 1978); in the digestive gland of Ruditapes phillipinarum (Sarasquete et al, 1992) and of Sunetta scripta (Mathew and Damodaran, 1997); and in the kidney of Tridacna gigas (Reid et al, 1984). As in E. exalbida, these are all tissues containing mitotic cells, which is in contrast to crustaceans, insects and vertebrates, where lipofuscin seems to be concentrated in postmitotic cell tissues such as nerves (Hammer and Braum, 1988;Sheehy et al, 1999;Bluhm and Brey, 2001).…”

Section: Is the Substance Found In E Exalbida Lipofuscin?mentioning

confidence: 99%

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

View full text Add to dashboard Cite

In the hard clam Eurhomalea exalbida, autofluorescent granules were detected in high concentrations in the connective tissue around the intestine and in other tissues. Autofluorescence combined with Sudan black B and PAS positive reactions suggested that these granules were lipofuscin-like. The concentration of this material in the connective tissue (CT) around the intestine was quantified by image analysis and expressed as total area fraction occupied by lipofuscin granules. Lipofuscin concentration was distinctly better related with individual age as determined from stable isotope-validated shell growth bands than with any morphometric parameter. This relationship was described best by a Gompertz model: Lipofuscin t = 24.79e À e À 0.029(Age t À 58.578) (N = 38; r nl 2 = 0.882). Age was predicted from lipofuscin CT concentration by a von Bertalanffy model: Age t = 68.00 (1 À e À 0.146(Lipofuscin t + 0.028) ) 0.664 (N = 38; r nl 2 = 0.933). Our findings suggest that lipofuscin CT concentration in E. exalbida is a function of individual age. If this holds true for bivalves in general, lipofuscin may be a suitable proxy for age in species with less clear shell growth band patterns. D

show abstract

“…Quinine sulphate at a concentration of 1 µg/ml in 0.1 M sulphuric acid was used as a standard for fluorescence intensity and wavelength calibration (Hammer and Braum, 1988). The production of LSFP were measured using excitation/emission wavelengths of 350/445 nm and 400/455 nm which are characteristic of lipid complexes formed by the cross linking of the aldehyde groups of MDA with amino groups of phosphatidylserine and phosphatidylethanolamine, respectively (Jain, 1988).…”

Section: Determination Of Malondialdehyde (Mda) and Lipid-soluble Flumentioning

confidence: 99%

Relationships between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentex dentex eggs and larvae

et al. 1999

View full text Add to dashboard Cite

The overall aim is the characterization of the antioxidant systems in marine fish during early development in order to enhance growth and quality of early life stages by avoiding oxidation problems that may cause pathologies and disease. The present study determined the activities of the antioxidant system enzymes and levels of vitamin E, lipid peroxidation products and fatty acids during early development without exogenous feeding in the marine fish, Dentex dentex. Samples of fertilized eggs just prior to hatch, newly hatched larvae and unfed larvae up to day 9 after hatch were analysed on a daily basis.The larvae did not grow during the experiment and by day 9 the surviving larvae weighed only a third of the newly hatched larvae. Lipid was a major energy source during this period of starvation as evidenced by greatly decreased amounts of total lipid due to a preferential utilisation of neutral lipids. All groups of fatty acids (saturated, monounsaturated and polyunsaturated) were consumed throughout the starvation period. Vitamin E levels were relatively constant until significantly declining by days 8 and 9. Similarly, levels of lipid-soluble fluorescent products (LSFP) were relatively constant until day 6 and then significantly decreased from days 7 to 9. In contrast, the level of malondialdehyde (MDA) was very high in eggs but was 3-fold lower in newly hatched larvae and then decreased over the whole time-course and was also significantly lower on days 8 and 9.The activities of enzymes of the antioxidation system were expressed in both eggs and early larvae and varied during the developmental period with levels of catalase and glutathione peroxidase (GPX) being initially low in eggs whereas glutathione-Stransferase (GST) and superoxide dismutase (SOD) were higher in eggs than in larvae.Overall, the activities of catalase and GPX increased, whereas GST and total SOD activities decreased, during the early stages of development during a period where there was no exogenous dietary input into the larvae. Glutathione reductase activity was very low and showed an increasing trend initially until days 2-3 before declining and varying over the remaining period.In conclusion, we determined the prooxidant (polyunsaturated fatty acid) status, antioxidant status (vitamin E), peroxidation status (MDA and LSFP) and the activities of the antioxidant enzyme systems in a single integrated study in unfed marine fish larvae during early development.

show abstract

Quantification of age pigments (lipofuscin)

Cited by 33 publications

References 161 publications

Use of extractable lipofuscin for age determination of blue crab Callinectes sapidus

Use of extractable lipofuscin for age determination of blue crab Callinectes sapidus

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Relationships between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentex dentex eggs and larvae

Contact Info

Product

Resources

About