Quantification of Catechin and Epicatechin in Foods by Enzymatic-Spectrophotometric Method with Tyrosinase

Dias, Tiago; Silva, Mara Reis; Damiani, Clarissa; Silva, Flávio Alves da

doi:10.1007/s12161-017-0955-6

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…The soluble phenolic profile of guarana powder is well known and reported in the literature [5,[31][32][33]. The main components are catechin and its isomer, epicatechin, and proanthocyanidin dimers.…”

Section: Insoluble-bound Phenolic Profilementioning

confidence: 97%

“…Both animal and human studies have found that the consumption of guarana powder was safe and caused no toxicity in small doses (30 mg/kg for animals and 200 mg/day for humans) [29,30]. While the composition of the soluble polyphenols from guarana is well established and reported [5,[31][32][33], there is a clear gap in the available literature regarding the insoluble-bound fraction as a source of bioactive compounds. Considering that the guarana powder is traditionally consumed as a whole, as opposed to a water-based extract, the IBPPs are also ingested and may contribute to the overall health effects of guarana consumption.…”

Section: Introductionmentioning

confidence: 99%

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Insoluble-Bound Polyphenols Released from Guarana Powder: Inhibition of Alpha-Glucosidase and Proanthocyanidin Profile

Acdc

Soares

et al. 2020

Molecules

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The Brazilian Food Supplement Law recently recognized that guarana (Paullinia cupana) contains bioactive substances, hence supporting its role as a functional food ingredient. The health benefits of guarana are associated, at least in part, to its phenolic compounds. However, to the best of our knowledge, there is no literature addressing the presence of phenolic compounds in the fraction containing insoluble-bound compounds and its contribution in terms of alpha-glucosidase inhibition. The concentration of phenolic extracts released from the insoluble-bound fraction required to inhibit 50% of alpha-glucosidase (IC50) activity was 5.8-fold lower than that present in the soluble counterpart. Both fractions exhibited a mixed inhibition mode. Fourteen proanthocyanidins (dimers to tetramers) present in the insoluble-bound fraction were tentatively identified by MALDi-TOF-MS. Future studies aiming at increasing the concentration of the soluble counterpart are deemed necessary. The results presented here enhance the phenolic database of guarana and have a practical impact on the procurement of nutraceuticals and functional ingredients related to the prevention and/or management of type 2 diabetes. The Brazilian normative on food supplements has been recently revised. This study lends support to the future inclusion of guarana powder in the list of sources of proanthocyanidins for the industry of food supplements.

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Section: Insoluble-bound Phenolic Profilementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Insoluble-Bound Polyphenols Released from Guarana Powder: Inhibition of Alpha-Glucosidase and Proanthocyanidin Profile

Acdc

Soares

et al. 2020

Molecules

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“…All the chemicals were used without any further purification step. [37,38], cyanocobalamin (vitamin B12) [41], and nicotinic acid [44].…”

Section: Chemicalsmentioning

confidence: 99%

“…Epicatechin (E) belongs to a particular category of polyphenols, the flavonoids, which are products of the secondary metabolism of plants [36]. Epicatechin is commonly found in a wide variety of foods, such as fruits (e.g., apples, grapes, cherries, and apricots), vegetables, legumes, cocoa derivatives, wine, and some teas [37,38]. This catechin has been shown to reduce hypertension, to improve the endothelial function [39], and is thought to enhance cognition [40].…”

Section: Introductionmentioning

confidence: 99%

“…Different biodegradable and ethyl-lactate-based ATPS with organic salts were applied at 298.15 K and 0.1 MPa in the partition of these biomolecules for the first time, in an effort to find effective extraction media which could promote a more sustainable production of therapeutics and cosmetics and a more circular economy. [37,38], cyanocobalamin (vitamin B12) [41], and nicotinic acid [44].…”

Section: Introductionmentioning

confidence: 99%

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Extraction of Polyphenols and Vitamins Using Biodegradable ATPS Based on Ethyl Lactate

2022

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The growing human population, together with the inefficient use of natural resources, has been dramatically increasing the production of food waste, which poses serious economic, environmental, and social problems. Being so, it is necessary to increase the efficiency of food consumption so as to reduce its waste and to convert the remaining residues into societal benefits. Since this biowaste is rich in polyphenols and vitamins, it could become the feedstock for the production of important value-added compounds for the pharmaceutical (e.g., food supplements) and cosmetic (e.g., creams and shampoos) industries. In this work, partition studies of one polyphenol (epicatechin) and two B-complex vitamins (cyanocobalamin and nicotinic acid) were performed in biodegradable Aqueous Two-Phase Systems (ATPS) based on ethyl lactate and on organic salts (disodium tartrate, tripotassium citrate, and trisodium citrate) at 298.15 K and 0.1 MPa. The largest partition coefficient (K) and extraction efficiency (E) were obtained for vitamin B12 (K=78.56, E=97.5%) for the longest tie line TLL=77.66% in the ATPS {ethyl lactate (1) + tripotassium citrate (2) + water (3)}. All the extractions were obtained with low biomolecule mass losses in quantification (<5%) and after a thorough study of pH influence in the UV–Vis absorbance spectra.

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Influence of a Cocoa-Enriched Diet on the Intestinal Immune System and Microbiota

Camps-Bossacoma

Massot-Cladera

Pérez-Cano

et al. 2019

Dietary Interventions in Gastrointestinal Diseases

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Quantification of Catechin and Epicatechin in Foods by Enzymatic-Spectrophotometric Method with Tyrosinase

Cited by 7 publications

References 32 publications

Insoluble-Bound Polyphenols Released from Guarana Powder: Inhibition of Alpha-Glucosidase and Proanthocyanidin Profile

Insoluble-Bound Polyphenols Released from Guarana Powder: Inhibition of Alpha-Glucosidase and Proanthocyanidin Profile

Extraction of Polyphenols and Vitamins Using Biodegradable ATPS Based on Ethyl Lactate

Influence of a Cocoa-Enriched Diet on the Intestinal Immune System and Microbiota

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