Quantification of Residual Clove Oil, Benzocaine and Tricaine in Fish Fillets Using Spe and Uplc-Dad

Abstract

Residual quantification of the anesthetics clove oil (CO) – isoeugenol (ISO), eugenol (EUG) and methyleugenol (MET) –,benzocaine (BZN) and tricaine (MS-222) was made in fillets of two fish species: Nile tilapia (Oreochromis niloticus) and acatfish hybrid, cachadia (Pseudoplatystoma reticulatum x Leiarius marmoratus). Samples (n=4) of each fish wereevaluated after submitted to anesthesia in five dosages defined based on the induction time of each species afterdepuration times (0h, 12h, 24h and 48h). Different methodologies of sample preparation were tested and selectedaccording to the better recovery. The quantification of anesthetics was performed by UPLC-DAD. The variance of residualmeans among anesthetics, dosages and fish species was compared. After anesthesia (0h) both species, tilapia andcachadia, presented residual anesthetics. Fishes depurated during 12h, 24h and 48h did not present detectable values, itmeans, values were below the limits of detection. BZN presented the highest mean residual concentration for tilapia andcachadia (p=0.01), while MS-222 presented the lowest residual amounts in tilapias and EUG in cachadias, what may berelated to the metabolism and carcass composition of each fish species. There were no significant differences among thefive dosages, except the lowest MS-222 concentration in tilapias that resulted in higher residual concentrations becauselow dosages increase the induction time and consequently the permanence of the fish in anesthesia. Ultimately, meanvalues of residues in cachadia were higher than in tilapia, and MS-222 and EUG presented the lowest residual values fortilapia and cachadia, respectively.

Determination of Benzocaine and Tricaine in Fish Fillets Using BIA with Amperometric Detection

“…Moreover, analytical frequency of 360 injections per hour for the benzocaine and 450 injections per hour for the tricaine were observe. Determination of residual clove oil, tricaine and benzocaine in fillets of two fish species (Nile tilapia and cachadia) using UPLC‐DA . In this study, samples (n=4) of each fish species were evaluated after submitted to anesthesia in different dosages and taking into account four depuration times (0 h, 12 h, 24 h and 48 h).…”