Quantitation of IgE Binding to the Chitinase and Chitinase-Like House Dust Mite Allergens Der p 15 and Der p 18 Compared to the Major and Mid-Range Allergens

Hales, Belinda J.; Elliot, Claire E.; Chai, Lee Ying; Pearce, Leigh; Tipayanon, Thitinun; Hazell, L.A.; Stone, Shane R.; Piboonpocanun, Surapon; Thomas, Wayne R.; Smith, Wendy-Anne

doi:10.1159/000339760

Cited by 22 publications

(20 citation statements)

References 70 publications

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“…A recent report demonstrated that asthmatic children had an expanded IgE repertoire with more recognized allergen components (Der p 1, Der p 2, Der p 5 and Der p 23) and increased sIgE levels than nonasthmatic children [30] . Other minor components contributed less to IgE binding quantity than Der p 1 and Der p 2 [31] . Kidon et al [17] have shown that sIgE responses to an increased number of distinct mite allergens correlates with the complexity of the allergic phenotype, and that both Der p 1 and Der p 2 are risk factors for multisystemic phenotypes of allergic diseases in Singapore.…”

Section: Discussionmentioning

confidence: 86%

Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

Wang

Gao

Zhou

et al. 2015

Int Arch Allergy Immunol

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Background: The role of specific IgE (sIgE) against Der p 1 and Der p 2 in Chinese patients with house dust mite (HDM) allergy has not yet been well investigated. Methods: Onehundred patients were enrolled, based on sensitization and doctor-diagnosed allergy to HDM. Questionnaires were administered to document demographic and clinical characteristics. Serum IgE reactivity to Dermatophagoides pteronyssinus (Dp) extract, Der p 1, Der p 2 and Der p 10 was measured by ImmunoCAP. Results: Almost all patients were sensitized to Der p 1 (95%) and Der p 2 (93%), with both allergens together being largely responsible for the total anti-HDM IgE response. No evidence for a significant role of Der p 10 was found. Overall, IgE responses to HDM and its 2 major allergens were higher in children than in adults in this cross-sectional study. With increasing age, IgE responses to Der p 2 become more important. A positive correlation was observed between the reaction of sIgE against Dp, Der p 1 and Der p 2 and the number of organs (including the eyes, nose, lungs and skin) that were affected in patients. Conclusions: In China, Der p 1 and Der p 2 are the dominant allergens in patients with HDM allergy. The relative importance of Der p 1 and Der p 2 changes with age, in favor of Der p 2. Overall, sIgE titers were positively associated with the number of organs affected.

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Section: Discussionmentioning

confidence: 86%

Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

Wang

Gao

Zhou

et al. 2015

Int Arch Allergy Immunol

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“…In addition, other replacements that seem to be relevant in the differential binding activity are two proline residues, only present in the second isoform, which are known to influence loop formation. Other HDM allergens such as the peritrophin Der p 23 [37] and the chitinase Der p 15 [42,43] also contain a CBD, but their sequence similarity with Blo t 12 was rather low. This is in agreement with the lack of reactive components to anti-Blo t 12-specific polyclonal and monoclonal antibodies in D. pteronyssinus extracts (Zakzuk et al, unpubl.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Chitin on the Immune Response to the House Dust Mite Allergen Blo t 12

Zakzuk

Benedetti

Fernández-Caldas

et al. 2013

Int Arch Allergy Immunol

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Background: Information about the biological properties of Blomia tropicalis allergens is scarce. It is predicted that Blo t 12, an allergen with two described isoforms, contains a chitin-binding domain, similar to that found in peritrophins. Th2 adjuvant properties have been described for chitin. Therefore, it is feasible that binding to this carbohydrate influences its allergenicity. We aimed to evaluate the chitin-binding activity of Blo t 12 isoallergens and its effect on airway inflammation and antibody responses in a murine model of allergen sensitization. Methods: Chitin-binding assays were conducted with the recombinant isoallergens Blo t 12.0101 and Blo t 12.0102. BALB/c mice were sensitized via i.p. with any of the two isoforms (alone, with chitin or alum) and then challenged intranasally. Methacholine-induced bronchial hyperreactivity was tested by whole-body plethysmography and lung sections were stained with hematoxylin and eosin and periodic-acid Schiff. Total IgE and allergen-specific IgE, IgG1 and IgG2 levels were measured by ELISA. Results: The two isoforms bound chitin, but Blo t 12.0101 showed a stronger binding capacity. Both isoforms induced total and allergen-specific IgE, airway hyperreactivity, bronchial inflammation and mucus secretion without any adjuvant; however, when administered with chitin, Blo t 12.0101 induced higher total IgE levels. The IgG1/IgG2a ratio was significantly higher in mice immunized with Blo t 12.0101 than those immunized with Blo t 12.0102. As peritrophins, Blo t 12 was detected in mite feces. Conclusions: Blo t 12 isoforms are chitin-binding proteins that induce airway inflammation and bronchial hyperreactivity. However, for Blo t 12.0101, chitin reinforces its effects on total IgE production.

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“…Two versions of the chip have been made, one which contains a collection of enterotoxins from Staphylococcus aureus involved in respiratory allergies [44] and another comprising an extended panel of antigens relevant for hypersensitivity to wheat [29,30,[45][46][47]. Considering the poor quality of natural house dust mite allergen extracts, we aimed to implement a panel of purified house dust mite allergens that is as complete as possible [48][49][50]. Furthermore, several cockroach allergens were included [51,52].…”

Section: Examples Of Detecting Early Allergen-specific Ige Responses mentioning

confidence: 99%

Advances in allergen-microarray technology for diagnosis and monitoring of allergy: The MeDALL allergen-chip

Lupinek

Wollmann

Baar

et al. 2014

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259

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Allergy diagnosis based on purified allergen molecules provides detailed information regarding the individual sensitization profile of allergic patients, allows monitoring of the development of allergic disease and of the effect of therapies on the immune response to individual allergen molecules. Allergen microarrays contain a large variety of allergen molecules and thus allow the simultaneous detection of allergic patients' antibody reactivity profiles towards each of the MeDALL is a European research program in which allergen microarray technology is used for the monitoring of the development of allergic disease in childhood, to draw a geographic map of the recognition of clinically relevant allergens in different populations and to establish reactivity profiles which are associated with and predict certain disease manifestations. We describe technical advances of the MeDALL allergen-chip regarding specificity, sensitivity and its ability to deliver test results which are close to in vivo reactivity. In addition, the usefulness and numerous advantages of allergen microarrays for allergy research, refined allergy diagnosis, monitoring of disease, of the effects of therapies, for improving the prescription of specific immunotherapy and for prevention are discussed. Europe PMC Funders Group

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Quantitation of IgE Binding to the Chitinase and Chitinase-Like House Dust Mite Allergens Der p 15 and Der p 18 Compared to the Major and Mid-Range Allergens

Cited by 22 publications

References 70 publications

Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

The Influence of Chitin on the Immune Response to the House Dust Mite Allergen Blo t 12

Advances in allergen-microarray technology for diagnosis and monitoring of allergy: The MeDALL allergen-chip

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