Quantitation of methylated basic amino acids in biological fluid

Lou, Marjorie F.; Siena, Michael

doi:10.1016/0006-2944(81)90089-2

Cited by 6 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are many analytical methods for the determination of ADMA including paper chromatography, thin layer chromatography, electrophoresis, ion-exchange chromatography and monoclonal antibody assay [6][7][8][9][10][11][12]. Reserved-phase HPLC has also been employed for the quantitative and qualitative analysis of methylated amino acids in plasma [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

The Measurement of Asymmetric Dimethylarginine in Human Plasma by High Performance Liquid Chromatography

Kurt¹,

Öztosun²,

Aydın³

et al. 2012

J Invest Biochem

View full text Add to dashboard Cite

Objective: We aimed to establish a high performance liquid chromatography (HPLC) method to measure plasma ADMA levels easily, sensitively and reliably. Methods: We used fluorescence detection, 50 mM sodium acetate buffer/ methanol/ Tetrahydrofuran as a mobile phase (A, 82:17:1; B, 22:77:1, % v, respectively), gradient method, 338 and 425 nm excitation and emission wavelengths, respectively. Samples were derivatizated with o-Phthaldialdehyde. Results: The recovery was found as 96 to 98,3 %. Intra-assay and inter-assay coefficients of variation (CV) were 2.3% and 2.7%, respectively. Conclusion: These findings suggest that our method is suitable for routine determination of ADMA more reliably.

show abstract