Quantitative analyses of the effect of silk fibroin/nano-hydroxyapatite composites on osteogenic differentiation of MG-63 human osteosarcoma cells

Lin, Linxue; Hao, Runsong; Xiong, Wei; Zhong, Jian

doi:10.1016/j.jbiosc.2014.10.009

Cited by 34 publications

(18 citation statements)

References 31 publications

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“…Neither nHAp nor BG pristine spectra show a vibration band around 1151, but Ch and C show it. nHAp's most intense band is in 1020 cm −1 , followed by the band at 600 cm −1 , as already observed in other works, where the 1010 to 1120 cm −1 bands are related to asymmetric stretching of P-O and the shoulder band at 964 cm −1 represents the symmetric stretching mode of P-O; also, the bands at 567 and 602 cm −1 are assigned to the asymmetric bending mode of O-P-O; all these vibration bands correspond to the PO 4 3− group [23][24][25]. Another work that synthetized nHAp in silica gels has assigned the bands found at 560 to 640, 963 and 1028 to 1110 cm −1 to the phosphate group [26].…”

Section: Ftir Spectrophotometrysupporting

confidence: 83%

Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

et al. 2021

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The development of organic–inorganic hybrid materials deserves special interest for bone tissue engineering applications, where materials must have properties that induce the survival and activation of cells derived from the mesenchyme. In this work, four bio-nanocomposites based on cellulose and variable content of chitosan, from 15 to 50 w% based on cellulose, with nanohydroxyapatite and β-Glycerophosphate as cross-linking agent were synthesized by simplified and low-energy-demanding solvent exchange method to determine the best ratio of chitosan to cellulose matrix. This study analyzes the metabolic activity and survival of human dermal fibroblast cells cultivated in four bio-nanocomposites based on cellulose and the variable content of chitosan. The biocompatibility was tested by the in vitro cytotoxicity assays Live/Dead and PrestoBlue. In addition, the composites were characterized by FTIR, XRD and SEM. The results have shown that the vibration bands of β-Glycerophosphate have prevailed over the other components bands, while new diffraction planes have emerged from the interaction between the cross-linking agent and the biopolymers. The bio-nanocomposite micrographs have shown no surface porosity as purposely designed. On the other hand, cell death and detachment were observed when the composites of 1 and 0.1 w/v% were used. However, the composite containing 10 w% chitosan, against the sum of cellulose and β-Glycerophosphate, has shown less cell death and detachment when used at 0.01 w/v%, making it suitable for more in vitro studies in bone tissue engineering, as a promising economical biomaterial.

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Section: Ftir Spectrophotometrysupporting

confidence: 83%

Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

et al. 2021

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“…mylitta SFNPs deposited over the titanium surface not only supported osteoblast adhesion, proliferation, and differentiation with respect to bare Ti surface but also attained sustained in vitro drug release . In another work, MG-63 human osteosarcoma cells had demonstrated better cell adhesion and osteogenic differentiation when cultured on SF/n-HA composites, deposited on Ti alloy substrate in comparison to nanohydroxyapatite alone . Reports have shown that the composite scaffolds of nanohydroxyapatite (nHAp) and silk fibroin (SF) promote bone regeneration via signaling pathways associated with cell-biomaterial interaction.…”

Section: Application Of Silk-based Nanomaterials For 3d Tissue Engine...mentioning

confidence: 99%

“…115 In another work, MG-63 human osteosarcoma cells had demonstrated better cell adhesion and osteogenic differentiation when cultured on SF/n-HA composites, deposited on Ti alloy substrate in comparison to nanohydroxyapatite alone. 116 Reports have shown that the composite scaffolds of nanohydroxyapatite (nHAp) and silk fibroin (SF) promote bone regeneration via signaling pathways associated with cell-biomaterial interaction. However, the role of soluble factors in osteoinduction with nHAp−SF has not been explored.…”

Section: Application Of Silk-based Nanomaterialsmentioning

confidence: 99%

Comprehensive Review on Silk at Nanoscale for Regenerative Medicine and Allied Applications

Mehrotra

Chouhan

Konwarh

et al. 2019

ACS Biomater. Sci. Eng.

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Materials at the nanoscale offer numerous avenues to be explored and exploited in diverse realms. Among others, proteinaceous biomaterials such as silk hold immense prospects in the domain of nanoengineering. Silk offers a unique combination of desirable facets like biocompatibility; extraordinary mechanical properties, such as elongation, elasticity, toughness, and modulus; and tunable biodegradability which are far better than most naturally occurring and engineered materials. Much of these properties are due to the molecular structure of the silk protein and it is self-assembly into hierarchical structures. Taking advantage of the hierarchical assembly, a large number of fabrication strategies have now emerged that allow the tailoring of silk structure of at the nanoscale. Harnessing the favorable properties of silk, such methods offer a promising direction toward producing structurally and functionally optimized silk nanomaterials. This review discusses the critical structure–property relationship in silk that occurs at the nanoscale and also aims to bring out the recent status in the approaches for fabrication, characterization, and the gamut of applications of various silk-based nanomaterials (nanoparticles, nanofibers, and nanocomposites) in the niche of translational research. Harnessing the favorable nanostructure of silk, the review also takes into account the impetus of silk in avant-garde applications such as chemo-biosensing, energy harvesting, microfluidics, and environmental applications.

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“…To investigate the osteogenic differentiation of the hBMSCs on the Ti specimens, the alkaline phosphatase (ALP) activity and the total protein content of the cells were measured using an ALP Activity Kit and a Protein Assay Kit (both from Invitrogen, USA), respectively, as reported previously [23]. Furthermore, the expression levels of osteogenic genes at 7 days after cell seeding were determined by quantitative real-time polymerization chain reaction (PCR) analysis [24] using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany), and primers specific for six gene markers: collagen type-1 (COL-1), osteocalcin (OCN), osteopontin (OPN), osteoprotegerin (OPG), runt-related transcription factor 2 (RUNX2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; internal control for mRNAs) (Supplemental data, Table S1).…”

Section: Test For Osteogenic Differentiationmentioning

confidence: 99%

Combined antibacterial and osteogenic in situ effects of a bifunctional titanium alloy with nanoscale hydroxyapatite coating

Liu

Wei

Deng

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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To resolve the problems of bacterial infections and the low efficiency of osteogenesis of implanted titanium alloys in clinical dental and bone therapy, we developed a bifunctional titanium alloy (Ti) with a nano-hydroxyapatite (HA) coating (HBD þ BMP/HA-Ti), which enables the sustained release of the natural antimicrobial peptide human b-defensin 3 (HBD-3) and bone morphogenetic protein-2 (BMP-2). Due to the poriferous nano-sized structure of the HA coating with a 20-30 lm thickness, the HBD þ BMP/HA-Ti material had a high encapsulation efficiency (>74%) and exhibited synchronized slow release of HBD-3 and BMP-2. In an antibacterial test, HBD þ BMP/HA-Ti prevented the growth of bacteria in an inoculated medium, and its surface remained free from viable bacteria after a continuous incubation with Gram-negative and Gram-positive strains for 7 days. Furthermore, good adhesion, proliferation and osteogenic differentiation of hBMSCs in contact with HBD þ BMP/HA-Ti were achieved in 7 days. Therefore, the bifunctional titanium alloy HBD þ BMP/HA-Ti has a great potential for eventual applications in the protection of implants against bacteria in the orthopaedic and dental clinic.

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Quantitative analyses of the effect of silk fibroin/nano-hydroxyapatite composites on osteogenic differentiation of MG-63 human osteosarcoma cells

Cited by 34 publications

References 31 publications

Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

Comprehensive Review on Silk at Nanoscale for Regenerative Medicine and Allied Applications

Combined antibacterial and osteogenic in situ effects of a bifunctional titanium alloy with nanoscale hydroxyapatite coating

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