Quantitative Changes in Uterine Nucleic Acids During the Oestrous Cycle in Rats

Heald, P. J.; O'grady, J. E.; Howie, A J

doi:10.1530/jrf.0.0210481

Cited by 17 publications

(7 citation statements)

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“…Samples (equivalent to 15mg wet wt. of tissue) for the determination of DNA and RNA were precipitated with HC104 during this procedure, and the radioactivity due to [3H]uridine remaining in the supernatant (acid-soluble fraction) was determined.Samples of the solutions and residues containing the separated nucleic acids (from 15mg of tissue) (Heald, O'Grady & Howie, 1970) were dried in vacuo and dissolved by overnight (18 h) incubation at 37°C in 0.4 ml of Hyamine hydroxide (hydroxide of Hyamine lOX; Packard Instrument Co. Inc., La Grange, Ill., U.S.A.). The solutions containing DNA, RNA and acid-soluble radioactivity were washed into counting vials with 14.6ml of butyl-PBD [5-(4-biphenylyl)-2-(4-tert.-butylphenyl)-1-oxa-3,4-diazole (British Drug Houses Ltd., Poole, Dorset, U.K.)] scintillator solution.…”

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The uptake of [3h] uridine into the nucleic acids of rat uterus during early pregnancy

Heald

O'grady

1969

Biochemical Journal

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1. Changes in content and uptake of [3H]uridine into the nucleic acids ofrat uterus during the first 9 days of pregnancy were studied. 2. From day 6 implantation sites were separated from the rest of the uterine tissue for independent analysis. 3. Up to day 5 of pregnancy no changes were found in the total dry matter or in RNA and DNA content/unit dry matter nor in the RNA/DNA ratios. 4. From day 6, when implantation sites are visible, the water content of the implantation sites increased by 2-3%, and the RNA content/unit dry wt. and the RNA/DNA ratios increased. The DNA content/unit dry wt. did not increase in the implantation sites until day 8. 5. Uptake of [3H]uridine into the acid-soluble fraction of the tissues was markedly higher in implantation sites than in non-implantation sites. 6. Uptake of [3H]uridine into RNA was significantly increased on day 3 ofpregnancy and again on day 5. 7. On days 6 and 7, the incorporation into RNA of implantation sites was significantly higher than in the remainder of the uterine tissue but decreased on days 8 and 9 to the same value as that of the normal tissue. 8. No change occurred in uptake into DNA until day 6, when there was an increase in uptake by the implantation sites. 9. It is suggested that the increase in RNA synthesis on day 3 is a preparation of the uterus for the onset of implantation on day 5, and that increased synthesis in implantation sites on days 6 and 7 is the elaboration of new RNA necessary for this early stage of pregnancy to commence.After a fertile mating in the rat, the fertilized ova mature and divide in the oviduct until, 3-3j days after mating, the blastocysts are shed into the uterus and by day 6 become attached to the uterine epithelial tissue. This latter process is known as implantation.Implantation has been extensively studied endocrinologically (cf. Mayer, 1965) and, since it is accompanied by a rapid growth and development of the uterus at the points of implantation, changes in DNA and RNA have been studied during this period in naturally mated pregnant and pseudopregnant rats (Miller, Owen & Emmens, 1968;Malinowska, Greenstreet & Fotherby, 1969), and in rats in which pseudopregnancy was induced by electrical stimulation of the cervix (Shelesnyak & Tic, 1963). It was shown that the content and concentration of RNA increased from days 5-6 of pregnancy, but that the concentration of DNA remained essentially constant. Moreover, incorporation of [3H]uridine into the total uterine RNA was increased on days 3-4 of pregnancy, declined (though not significantly) on day 5 and again increased steadily from day 6 onwards (Miller et al. 1968).However, measurements of change in total 3 uterine content do not permit any analysis of the more detailed events that are occurring. Thus from midnight on day 5 of pregnancy the sites of implantation are clearly visible and different from those of the surrounding uterine tissue (Psychoyos, 1963 Then 50 min later the animals were again lightly anaesthetized and 2.0ml of a solution of Niagara Blue 2B (2%, w/v,...

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“…Up to day5 the uteri were cut into small segments with scissors, and representative segments from each horn were taken for the determination of dry weight and of DNA and RNA as described by Heald, O'Grady & Howie, 1970. Samples (equivalent to 15mg wet wt.…”

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The uptake of [3h] uridine into the nucleic acids of rat uterus during early pregnancy

Heald

O'grady

1969

Biochemical Journal

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“…The nucleic acid composition of the uterus depends closely on hormonal control (Heald et al 1970;Miller 1976). Further, Little and Lambourne (1976) and Cox et al (1978) have demonstrated that the uterine RNA/DNA ratio is a sensitive indicator of the degree of oestrogenic stimulation in ovariectomized ewes.…”

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“…Since the exact nature of the altered responsiveness to oestrogen in ewes with permanent clover infertility is not clear, the following studies were carried Qut to try to elucidate this problem. The ratio of RNA to DNA in the uterus is a sensitive indication of oestrogenic stimulation (Heald et al 1970;Little and Lambourne 1976;Miller 1976) and was used to determine whether there was any abnormality in responsiveness to oestrogen in the uterus of clover-affected ewes.…”

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confidence: 99%

Proportions of Nucleic Acids in the Uteri of Ewes with Clover Disease and the Effect of Oestrogen after Ovariectomy

Tang¹,

Adams²

1982

Aust. Jnl. Of Bio. Sci.

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In entire ewes, the ratio of RNA to DNA in the uterus was higher at oestrus than at dioestrus but did not differ between control and clover-affected ewes at either stage of the oestrous cycle.After ovariectomy the ratio of RNA to DNA in the uteri decreased in both control and cloveraffected ewes, but the decrease was greater in the control ewes (P < 0·01). Subsequently when ewes received a single injection of oestradiol-17 p, a similar rise in uterine RNA/DNA ratio occun:ed in the two groups. However, following injection of the ewes with oestradiol-17 p for 3 days, the ratio of uterine RNA to DNA was higher in control than in clover-affected ewes (P < 0·05). Thus, the uteri from clover-affected ewes undergo less regression after ovariectomy and yet show less response to repeated oestrogenic stimulation than do those of control ewes. This pattern of response is consistent with the hypothesis that the permanent changes caused by phyto-oestrogens in ewes are analogous to the differentiation which normally occurs during organogenesis.

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“…Thus, a simple s.c. injection of 1.25 mg of testosterone propionate administered to female rats during the first 5 days of live induced persistent estrus in all animals. 39,41,42 These animals in adulthood present anovulation, higher LH secretion, polycystic ovaries and aggressive behavior when in contact with males, thus there is a resemblance to the human condition of polycystic ovary syndrome. 18,40 Furthermore, rats in persistent estrus may have hypertension, as shown in a study conducted by Gontijo et al 8 Those authors showed that rats in persistent estrus had a significant increase in arterial pressure, in comparison to rats in the control group.…”

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confidence: 99%

Current aspects of polycystic ovary syndrome: A literature review

Andrade

Mata

Borges³

et al. 2016

Rev. Assoc. Med. Bras.

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Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine disorder with variable prevalence, affecting about one in every 15 women worldwide. The diagnosis of polycystic ovary syndrome requires at least two of the following criteria: oligoovulation and/or anovulation, clinical and/or biochemical evidence of hyperandrogenism and morphology of polycystic ovaries. Women with PCOS appear to have a higher risk of developing metabolic disorders, hypertension and cardiovascular disorders. The aim of this article was to present a review of the literature by searching the databases Pubmed and Scielo, focusing on publications related to polycystic ovaries, including its pathogenesis, clinical manifestations, diagnosis and therapeutic aspects, as well as its association with cardiovascular and arterial hypertensive disorders.

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Quantitative Changes in Uterine Nucleic Acids During the Oestrous Cycle in Rats

Abstract: Summary. Changes in levels of RNA and DNA in rat uterus have been measured throughout the oestrous cycle in two strains of rats by a method designed specifically for application to uterine tissue. It has been shown that there are significant cyclic variations of RNA and

Cited by 17 publications

References 6 publications

The uptake of [3h] uridine into the nucleic acids of rat uterus during early pregnancy

The uptake of [3h] uridine into the nucleic acids of rat uterus during early pregnancy

Proportions of Nucleic Acids in the Uteri of Ewes with Clover Disease and the Effect of Oestrogen after Ovariectomy

Current aspects of polycystic ovary syndrome: A literature review

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