Quantitative determination of flavins – complex analyte/matrix effects in the presence of food colour additives

Sensitive determination of rose bengal in brown sugar by a molecularly imprinted solid-phase extraction monolithic capillary column coupled with capillary electrophoresis

“…Molecularly imprinted polymers (MIPs) are artificial recognition materials with 1 cavities, which are formed by polymerization of functional monomers and 2 cross-linkers around a target molecule after template removal to specifically bind the 3 molecule [12,13]. They allow both sample pretreatment and enrichment and have 4 been used in separation media [14], mimicking antibody [15] as well as chemical and 5 biochemical sensing [16].…”

“…Subsequently, the capillary 1 column was placed in a temperature-controlled water bath at 55 °C for 4.5 h, and then 2 washed by a micro-syringe pump with methanol/ammonium hydroxide (70:30, v/v) at 3 a flow rate of 0.1 mL min -1 to remove the unreacted reagents and redundant templates 4 until rose bengal could not be detected in the effluent by the CE system. 1 Thus the concentrations of rose bengal will be decreased when flowing out from the 2 column, which lead to the higher recovery. capacity of the column is, the more rose bengal will be captured inside the column.…”

“…4 (the red line), 19 however, rose bengal is detectable by using the MISPE column and the peaks of Table 3). 22 Page 12 of 26 Analytical Methods 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 For comparison, HPLC analysis was also performed on a Shimadzu LC-10A 1 HPLC system (Shimadzu, Kyoto, Japan) consisting of one LC-10ATVP pump and a 2 Shimadzu RF-10AXL LIF detector. 13 Therefore, this method showed good precision even at low concentrations.…”