Quantitative determination of low molecular weight carboxylic acids by capillary zone electrophoresis/conductivity detection

Abstract

Low molecular weight carboxylic acids are separated and quantitated by capillary zone electrophoresis (CZE) with an on-column conductivity detector. The addition of 0.2-0.5 mM TTAB (tetradecyltrimethylammonlum bromide) controls the electroosmotlc flow so that all carboxylate anions pass through the detector. Unlike other CZE detection methods, conductivity detection shows a direct relationship between retention time and peak area. This confers on conductivity detection, In CZE, the unique advantage that use of an Internal standard allows accurate determination of absolute concentrations In a mixture without separate calibration of response for each component.

The Utility of 4-(2-Thienyl)Pyridines as a Derivatization Reagent for HPLC and CE

“…Although no description on the determination of 13 by CE has been made, the present results were superior in linear ranges and plate number and similar in the lower detection limits to those for short-chain carboxylic acids reported by Zare, et al [3]. Determination of aldehydes and ketones with fluorescent Girard reagent 5 using CE.…”

“…using HPLC nr CE(capillary zone electrophoresis): 5-(4-pyridyl)-2-thiophenecarbaidehyoe(1 ), 5-(4-pyridyl)-2-thiophenemethanol(2 ), 4-(5-diazomethyl-2-thienyl)pyridine(3 ), 1-(2-ammonioethyl)-4-(2-thienyl)pyridinium dibromide(4), and 4-(2-thienyl)pyridinium acetohydrazide chloride(s). Detection limits and linear ranges for most of analytes were superior to those reported previously [2][3].…”

L'analyse des acides gras en électrophorèse capillaire

“…X. Huang et al, en 1989, ont été parmi les premiers à utiliser l'élec-trophorèse capillaire de zone (CZE) pour l'analyse d'acides carboxyliques de faibles masses moléculaires (C 1 -C 7 ) contenus dans des échantillons de vins blanc ou rouge [17]. Le tableau I regroupe les différentes conditions expérimen-tales utilisées en fonction des caractéristiques de la chaîne grasse.…”

Determinação simultânea de ânions por eletroforese capilar: características e aplicações

“…Neste trabalho, X. Huang e colaboradores 23 demonstraram a separação de uma mistura de ácidos carboxílicos de cadeia normal de 1 a 6 carbonos, em três condições. Em (A), a separação é conduzida sob fluxo eletroosmótico normal, ou seja, fluxo direcionado ao catodo (pólo aterrado), representado pelo vetor velocidade v osm .…”