Quantitative Determination of Metformin Hydrochloride in Tablet Formulation Containing Croscarmellose Sodium as Disintegrant by HPLC and UV Spectrophotometry

Umapathi, P.; Ayyappan, J; Quine, Sd Darlin

doi:10.4314/tjpr.v11i1.14

Cited by 43 publications

(23 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our literature survey verified that determination of metformin has been carried out in tablets by high-performance liquid chromatography (HPLC) [3][4][5][6], in human plasma using ionpair HPLC [7], or through using capillary electrophoresis [8,9].…”

Section: Introductionsupporting

confidence: 56%

Rapid RP-HPLC method for simultaneous estimation of metformin, pioglitazone, and glimepiride in human plasma

Sebaiy

El-Adl

Baraka

et al. 2020

Acta Chromatographica

View full text Add to dashboard Cite

An isocratic reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed for rapid and simultaneous separation and estimation of 3 antidiabetic drugs, namely, metformin, pioglitazone, and glimepiride, in human plasma within 3 min. Separation was carried out on a MAGELLEN 5U C18 (5 μm, 150 mm × 4.60 mm) using a mobile phase of MeOH–0.025 M KH2PO4 adjusted to pH 3.20 using ortho-phosphoric acid (85:15, v/v) at ambient temperature. The flow rate was 1 mL/min, and the maximum absorption was measured at 235 nm. The retention time of metformin, pioglitazone, and glimepiride was noted to be 1.24, 2.32, and 2.77 min, respectively, indicating a very short analysis time compared to that of other reported methods. Also, limits of detection were reported to be 0.05, 0.26, and 0.10 μg/mL for metformin, pioglitazone, and glimepiride, respectively, showing a high degree of method sensitivity. The method was then validated according to the FDA guidelines for the determination of the three drugs clinically in human plasma, in particular, regarding pharmacokinetic and bioequivalence simulation studies.

show abstract

Section: Introductionsupporting

confidence: 56%

Rapid RP-HPLC method for simultaneous estimation of metformin, pioglitazone, and glimepiride in human plasma

Sebaiy

El-Adl

Baraka

et al. 2020

Acta Chromatographica

View full text Add to dashboard Cite

show abstract

“…Determination of pharmaceuticals can be performed by various techniques, icluding highperformance liquid chromatography (HPLC) [11,12] and gas chromatography-mass spectrometry (GC-MS) [13,14]. HPLC is the most common method used for separation and determination of these compounds because most pharmaceuticals are non-volatile.…”

Section: Introductionmentioning

confidence: 99%

Development of Analytical Method for Detection of Some Pharmaceuticals in Surface Water

Al-Qaim¹,

Abdullah²,

Othman³

et al. 2013

Trop. J. Pharm Res

View full text Add to dashboard Cite

“…It is the cornerstone for the treatment of Diabetes Mellitus type II, where it is used alone or in combination with other antidiabetic classes like sulfonylurea's, alphaglycosidase inhibitors, or insulin [3]. Our literature survey verified that determination of metformin has been carried out using HPLC in tablets [4][5][6][7][8], in human plasma [9,10] or through using capillary electrophoresis [11,12]. Gliclazide (GLC ), is a second generation sulphonylurea drug which is chemically, 1-(3-Azabicyclo[3.3.0] oct-3-yl)-3-(p-tolylsulfonyl)urea ( Fig.…”

Section: Metforminmentioning

confidence: 83%

Rapid RP-HPLC Method for Simultaneous Estimation of Some Antidiabetics; Metformin, Gliclazide and Glimepiride in Tablets.

et al. 2018

View full text Add to dashboard Cite

A N ISOCRATIC RP-HPLC method has been developed for rapid and simultaneous separation and estimation of three antidiabetics drugs, metformin, gliclazide and glimepiride in tablet dosage forms within 6 minutes. Separation was carried out on a Thermo Scientific ® BDS Hypersil C 8 column (5µm, 2.50 x 4.60 mm) using a mobile phase of MeOH : 0.025M KH 2 PO 4 adjusted to pH 3.20 using ortho-phosphoric acid (70: 30, v/v) at ambient temperature. The flow rate was 1 mL/min and UV detection was set at 235 nm. The retention time of metformin, gliclazide and glimepiride was noted to be 3.06, 4.33 and 6.00 minutes respectively, indicating a very short analysis time rather than other reported methods. Also, limits of detection were reported to be 0.05, 1.21 and 0.11 µg/mL for metformin, gliclazide and glimepiride, respectively, showing a high degree of the method sensitivity. The method was then validated according to ICH guidelines where it was found to be accurate, reproducible and robust. Finally, the method was compared statistically with reference methods indicating that there is no significant difference between them in respect of precision and accuracy.

show abstract

Quantitative Determination of Metformin Hydrochloride in Tablet Formulation Containing Croscarmellose Sodium as Disintegrant by HPLC and UV Spectrophotometry

Cited by 43 publications

References 8 publications

Rapid RP-HPLC method for simultaneous estimation of metformin, pioglitazone, and glimepiride in human plasma

Rapid RP-HPLC method for simultaneous estimation of metformin, pioglitazone, and glimepiride in human plasma

Development of Analytical Method for Detection of Some Pharmaceuticals in Surface Water

Rapid RP-HPLC Method for Simultaneous Estimation of Some Antidiabetics; Metformin, Gliclazide and Glimepiride in Tablets.

Contact Info

Product

Resources

About