Quantitative determination of methanogenic cells based on analysis of ether-linked glycerolipids by high-performance liquid chromatography

Demizu, Kazuhiro; Ohtsubo, Satoshi; Kohno, Shuhei; Miura, Isao; Nishihara, Masateru; Koga, Yosuke

doi:10.1016/0922-338x(92)90553-7

Cited by 12 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Excision of polar head groups was performed according to the method of Demizu et al . [33]. The precipitated polar lipids were subjected to acetolysis at 165 °C for 20 h in 5 mL of a mixture of acetic acid/acetic anhydride (3 : 2, v/v).…”

Section: Methodsmentioning

confidence: 99%

Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus

et al. 2007

View full text Add to dashboard Cite

Complete saturation of the geranylgeranyl groups of biosynthetic intermediates of archaeal membrane lipids is an important reaction that confers chemical stability on the lipids of archaea, which generally inhabit extreme conditions. An enzyme encoded by the AF0464 gene of a hyperthermophilic archaeon, Archaeoglobus fulgidus, which is a distant homologue of plant geranylgeranyl reductases and an A. fulgidus menaquinone‐specific prenyl reductase[Hemmi H, Yoshihiro T, Shibuya K, Nakayama T, & Nishino T (2005) J Bacteriol187, 1937–1944], was recombinantly expressed and purified, and its geranylgeranyl reductase activity was examined. The radio HPLC analysis indicated that the flavoenzyme, which binds FAD noncovalently, showed activity towards lipid‐biosynthetic intermediates containing one or two geranylgeranyl groups under anaerobic conditions. It showed a preference for 2,3‐di‐O‐geranylgeranylglyceryl phosphate over 3‐O‐geranylgeranylglyceryl phosphate and geranylgeranyl diphosphate in vitro, and did not reduce the prenyl group of respiratory quinones in Escherichia coli cells. The substrate specificity strongly suggests that the enzyme is involved in the biosynthesis of archaeal membrane lipids. GC‐MS analysis of the reaction product from 2,3‐di‐O‐geranylgeranylglyceryl phosphate proved that the substrate was converted to archaetidic acid (2,3‐di‐O‐phytanylglyceryl phosphate). The archaeal enzyme required sodium dithionite as the electron donor for activity in vitro, similarly to the menaquinone‐specific prenyl reductase from the same anaerobic archaeon. On the other hand, in the presence of NADPH (the preferred electron donor for plant homologues), the enzyme reaction did not proceed.

show abstract

Section: Methodsmentioning

confidence: 99%

Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The first step is to use the modified Bligh and Dyer technique (e.g., Lai et al, 2008) or Soxhlet extraction (e.g., Lo and Chang, 1990) to obtain the total lipid extract. Separation of BTL, in the form of either intact lipids or hydrophobic cores or hydrocarbon chains, from the total lipid extract can be done on thin layer chromatography (TLC) (Lai et al, 2008;Tarui et al, 2007), column chromatograph , gas chromatography (GC) or GC-MS (Pearson et al, 2004;Shimada et al, 2002Shimada et al, , 2008Tarui et al, 2007) and high-performance liquid chromatography (HPLC) (Demizu et al, 1992;Hopmans et al, 2000;Huguet et al, 2006;Shimada et al, 2002).…”

Section: Isolation Of Btl From the Archaeamentioning

confidence: 99%

Archaebacterial bipolar tetraether lipids: Physico-chemical and membrane properties

Chong

2010

Chemistry and Physics of Lipids

128

117

View full text Add to dashboard Cite

“…Archaeal diether core lipids have been proposed as a methanogen biomarker or to estimate quantitatively methanogen biomass in natural systems. Respective studies have predominantly been restricted to highly anaerobic environments exhibiting significant CH 4 emissions such as rice paddies [16, 17], digester sludge [18, 19], marine sediment [20], faecal material [21], permafrost [22, 23], and peatlands [24–26]. Wachinger et al [27] observed that absolute Archaea cell numbers in mineral soils, calculated using the approximate ether lipid concentration 2.5 μ mol g −1 dry weight of methanogen cells [28], also reflected CH 4 productivity.…”

Section: Introductionmentioning

confidence: 99%

Archaeol: An Indicator of Methanogenesis in Water-Saturated Soils

Lim

Pancost

Hornibrook

et al. 2012

Archaea

View full text Add to dashboard Cite

Oxic soils typically are a sink for methane due to the presence of high-affinity methanotrophic Bacteria capable of oxidising methane. However, soils experiencing water saturation are able to host significant methanogenic archaeal communities, potentially affecting the capacity of the soil to act as a methane sink. In order to provide insight into methanogenic populations in such soils, the distribution of archaeol in free and conjugated forms was investigated as an indicator of fossilised and living methanogenic biomass using gas chromatography-mass spectrometry with selected ion monitoring. Of three soils studied, only one organic matter-rich site contained archaeol in quantifiable amounts. Assessment of the subsurface profile revealed a dominance of archaeol bound by glycosidic headgroups over phospholipids implying derivation from fossilised biomass. Moisture content, through control of organic carbon and anoxia, seemed to govern trends in methanogen biomass. Archaeol and crenarchaeol profiles differed, implying the former was not of thaumarcheotal origin. Based on these results, we propose the use of intact archaeol as a useful biomarker for methanogen biomass in soil and to track changes in moisture status and aeration related to climate change.

show abstract

Quantitative determination of methanogenic cells based on analysis of ether-linked glycerolipids by high-performance liquid chromatography

Cited by 12 publications

References 21 publications

Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus

Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus

Archaebacterial bipolar tetraether lipids: Physico-chemical and membrane properties

Archaeol: An Indicator of Methanogenesis in Water-Saturated Soils

Contact Info

Product

Resources

About